Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species
Abstract The evolution of phenotypic plasticity plays an essential role in adaptive responses to climate change; however, its regulatory mechanisms in marine organisms which exhibit high phenotypic plasticity still remain poorly understood. The temperature-responsive trait oleic acid content and its...
Published in: | Molecular Biology and Evolution |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2023
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1093/molbev/msad015 https://academic.oup.com/mbe/advance-article-pdf/doi/10.1093/molbev/msad015/48804016/msad015.pdf https://academic.oup.com/mbe/article-pdf/40/2/msad015/49318654/msad015.pdf |
id |
croxfordunivpr:10.1093/molbev/msad015 |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1093/molbev/msad015 2024-05-19T07:39:20+00:00 Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species Wang, Chaogang Li, Ao Cong, Rihao Qi, Haigang Wang, Wei Zhang, Guofan Li, Li Wittkopp, Patricia National Key R&D Program of China National Natural Science Foundation of China Strategic Priority Research Program of the Chinese Academy of Sciences Key Research and Development Program of Shandong Strategic Priority Research Program of the Chinese Academy of Sciences Young Elite Scientists Sponsorship Program China Association of Science and Technology Key Technology Research and Industrialization Demonstration Projects of Qingdao Technology and the Modern Agroindustry Technology Research System 2023 http://dx.doi.org/10.1093/molbev/msad015 https://academic.oup.com/mbe/advance-article-pdf/doi/10.1093/molbev/msad015/48804016/msad015.pdf https://academic.oup.com/mbe/article-pdf/40/2/msad015/49318654/msad015.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Molecular Biology and Evolution volume 40, issue 2 ISSN 0737-4038 1537-1719 journal-article 2023 croxfordunivpr https://doi.org/10.1093/molbev/msad015 2024-04-25T07:58:17Z Abstract The evolution of phenotypic plasticity plays an essential role in adaptive responses to climate change; however, its regulatory mechanisms in marine organisms which exhibit high phenotypic plasticity still remain poorly understood. The temperature-responsive trait oleic acid content and its major gene stearoyl-CoA desaturase (Scd) expression have diverged in two allopatric congeneric oyster species, cold-adapted Crassostrea gigas and warm-adapted Crassostrea angulata. In this study, genetic and molecular methods were used to characterize fatty acid desaturation and membrane fluidity regulated by oyster Scd. Sixteen causative single-nucleotide polymorphisms (SNPs) were identified in the promoter/cis-region of the Scd between wild C. gigas and C. angulata. Further functional experiments showed that an SNP (g.-333C [C. gigas allele] >T [C. angulata allele]) may influence Scd transcription by creating/disrupting the binding motif of the positive trans-factor Y-box factor in C. gigas/C. angulata, which mediates the higher/lower constitutive expression of Scd in C. gigas/C. angulata. Additionally, the positive trans-factor sterol-regulatory element–binding proteins (Srebp) were identified to specifically bind to the promoter of Scd in both species, and were downregulated during cold stress in C. gigas compared to upregulated in C. angulata. This partly explains the relatively lower environmental sensitivity (plasticity) of Scd in C. gigas. This study serves as an experimental case to reveal that both cis- and trans-variations shape the diverged pattern of phenotypic plasticity, which provides new insights into the formation of adaptive traits and the prediction of the adaptive potential of marine organisms to future climate change. Article in Journal/Newspaper Crassostrea gigas Oxford University Press Molecular Biology and Evolution |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract The evolution of phenotypic plasticity plays an essential role in adaptive responses to climate change; however, its regulatory mechanisms in marine organisms which exhibit high phenotypic plasticity still remain poorly understood. The temperature-responsive trait oleic acid content and its major gene stearoyl-CoA desaturase (Scd) expression have diverged in two allopatric congeneric oyster species, cold-adapted Crassostrea gigas and warm-adapted Crassostrea angulata. In this study, genetic and molecular methods were used to characterize fatty acid desaturation and membrane fluidity regulated by oyster Scd. Sixteen causative single-nucleotide polymorphisms (SNPs) were identified in the promoter/cis-region of the Scd between wild C. gigas and C. angulata. Further functional experiments showed that an SNP (g.-333C [C. gigas allele] >T [C. angulata allele]) may influence Scd transcription by creating/disrupting the binding motif of the positive trans-factor Y-box factor in C. gigas/C. angulata, which mediates the higher/lower constitutive expression of Scd in C. gigas/C. angulata. Additionally, the positive trans-factor sterol-regulatory element–binding proteins (Srebp) were identified to specifically bind to the promoter of Scd in both species, and were downregulated during cold stress in C. gigas compared to upregulated in C. angulata. This partly explains the relatively lower environmental sensitivity (plasticity) of Scd in C. gigas. This study serves as an experimental case to reveal that both cis- and trans-variations shape the diverged pattern of phenotypic plasticity, which provides new insights into the formation of adaptive traits and the prediction of the adaptive potential of marine organisms to future climate change. |
author2 |
Wittkopp, Patricia National Key R&D Program of China National Natural Science Foundation of China Strategic Priority Research Program of the Chinese Academy of Sciences Key Research and Development Program of Shandong Strategic Priority Research Program of the Chinese Academy of Sciences Young Elite Scientists Sponsorship Program China Association of Science and Technology Key Technology Research and Industrialization Demonstration Projects of Qingdao Technology and the Modern Agroindustry Technology Research System |
format |
Article in Journal/Newspaper |
author |
Wang, Chaogang Li, Ao Cong, Rihao Qi, Haigang Wang, Wei Zhang, Guofan Li, Li |
spellingShingle |
Wang, Chaogang Li, Ao Cong, Rihao Qi, Haigang Wang, Wei Zhang, Guofan Li, Li Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
author_facet |
Wang, Chaogang Li, Ao Cong, Rihao Qi, Haigang Wang, Wei Zhang, Guofan Li, Li |
author_sort |
Wang, Chaogang |
title |
Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
title_short |
Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
title_full |
Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
title_fullStr |
Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
title_full_unstemmed |
Cis- and Trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species |
title_sort |
cis- and trans-variations of stearoyl-coa desaturase provide new insights into the mechanisms of diverged pattern of phenotypic plasticity for temperature adaptation in two congeneric oyster species |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/molbev/msad015 https://academic.oup.com/mbe/advance-article-pdf/doi/10.1093/molbev/msad015/48804016/msad015.pdf https://academic.oup.com/mbe/article-pdf/40/2/msad015/49318654/msad015.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Molecular Biology and Evolution volume 40, issue 2 ISSN 0737-4038 1537-1719 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/molbev/msad015 |
container_title |
Molecular Biology and Evolution |
_version_ |
1799478894585184256 |