Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms
Abstract Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favorable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological su...
Published in: | Molecular Biology and Evolution |
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Oxford University Press (OUP)
2020
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croxfordunivpr:10.1093/molbev/msaa182 2024-05-19T07:48:57+00:00 Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms Vancaester, Emmelien Depuydt, Thomas Osuna-Cruz, Cristina Maria Vandepoele, Klaas Battistuzzi, Fabia Ursula BOF 2020 http://dx.doi.org/10.1093/molbev/msaa182 http://academic.oup.com/mbe/advance-article-pdf/doi/10.1093/molbev/msaa182/33737000/msaa182.pdf http://academic.oup.com/mbe/article-pdf/37/11/3243/34445562/msaa182.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Molecular Biology and Evolution volume 37, issue 11, page 3243-3257 ISSN 0737-4038 1537-1719 journal-article 2020 croxfordunivpr https://doi.org/10.1093/molbev/msaa182 2024-05-02T09:30:19Z Abstract Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favorable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3–5% of their proteome has a horizontal origin and a large influx occurred at the ancestor of diatoms. More than 90% of HGT genes are expressed, and species-specific HGT genes in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from HGT are implicated in several processes including environmental sensing and expand the metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor for roughly half of the diatoms and is only produced by bacteria. Five consecutive genes involved in the final synthesis of the cobalamin biosynthetic pathway, which could function as scavenging and repair genes, were detected as HGT. The full suite of these genes was detected in the cold-adapted diatom Fragilariopsis cylindrus. This might give diatoms originating from the Southern Ocean, a region typically depleted in cobalamin, a competitive advantage. Overall, we show that HGT is a prevalent mechanism that is actively used in diatoms to expand its adaptive capabilities. Article in Journal/Newspaper Southern Ocean Oxford University Press Molecular Biology and Evolution 37 11 3243 3257 |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favorable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3–5% of their proteome has a horizontal origin and a large influx occurred at the ancestor of diatoms. More than 90% of HGT genes are expressed, and species-specific HGT genes in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from HGT are implicated in several processes including environmental sensing and expand the metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor for roughly half of the diatoms and is only produced by bacteria. Five consecutive genes involved in the final synthesis of the cobalamin biosynthetic pathway, which could function as scavenging and repair genes, were detected as HGT. The full suite of these genes was detected in the cold-adapted diatom Fragilariopsis cylindrus. This might give diatoms originating from the Southern Ocean, a region typically depleted in cobalamin, a competitive advantage. Overall, we show that HGT is a prevalent mechanism that is actively used in diatoms to expand its adaptive capabilities. |
author2 |
Battistuzzi, Fabia Ursula BOF |
format |
Article in Journal/Newspaper |
author |
Vancaester, Emmelien Depuydt, Thomas Osuna-Cruz, Cristina Maria Vandepoele, Klaas |
spellingShingle |
Vancaester, Emmelien Depuydt, Thomas Osuna-Cruz, Cristina Maria Vandepoele, Klaas Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
author_facet |
Vancaester, Emmelien Depuydt, Thomas Osuna-Cruz, Cristina Maria Vandepoele, Klaas |
author_sort |
Vancaester, Emmelien |
title |
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
title_short |
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
title_full |
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
title_fullStr |
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
title_full_unstemmed |
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms |
title_sort |
comprehensive and functional analysis of horizontal gene transfer events in diatoms |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1093/molbev/msaa182 http://academic.oup.com/mbe/advance-article-pdf/doi/10.1093/molbev/msaa182/33737000/msaa182.pdf http://academic.oup.com/mbe/article-pdf/37/11/3243/34445562/msaa182.pdf |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Molecular Biology and Evolution volume 37, issue 11, page 3243-3257 ISSN 0737-4038 1537-1719 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/molbev/msaa182 |
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Molecular Biology and Evolution |
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37 |
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11 |
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3243 |
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3257 |
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1799467356978675712 |