Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.)
ABSTRACT Background Feeding-induced cell signaling and metabolic responses affect utilization of dietary nutrients but are rarely taken advantage of to improve animal nutrition. Objectives We hypothesized that by modulating postprandial kinetics and signaling, improved dietary utilization and growth...
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Online Access: | http://dx.doi.org/10.1093/jn/nxab221 http://academic.oup.com/jn/article-pdf/151/10/2957/40494640/nxab221.pdf |
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croxfordunivpr:10.1093/jn/nxab221 2023-05-15T18:15:46+02:00 Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) Wang, Ning Zhang, Xuemin Liu, Chengdong Wang, Xuan Zhou, Huihui Mai, Kangsen He, Gen National Key Research and Development Program of China National Natural Science Foundation of China National Laboratory for Marine Science and Technology Key R&D Program in Shandong Province China Agriculture Research System 2021 http://dx.doi.org/10.1093/jn/nxab221 http://academic.oup.com/jn/article-pdf/151/10/2957/40494640/nxab221.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model The Journal of Nutrition volume 151, issue 10, page 2957-2966 ISSN 0022-3166 1541-6100 Nutrition and Dietetics Medicine (miscellaneous) journal-article 2021 croxfordunivpr https://doi.org/10.1093/jn/nxab221 2022-09-30T10:05:41Z ABSTRACT Background Feeding-induced cell signaling and metabolic responses affect utilization of dietary nutrients but are rarely taken advantage of to improve animal nutrition. Objectives We hypothesized that by modulating postprandial kinetics and signaling, improved dietary utilization and growth performance could be achieved in animals. Methods Juvenile turbot (Scophthalmus maximus L.) with an initial mean ± SD weight of 10.1 ± 0.01 g were used. Two feeding frequencies (FFs), either 1 or 3 meals/d at a fixed 2.4% daily body weight ration, and 2 diets that were or were not supplemented with 1% crystalline leucine (Leu), were used in the 10-wk feeding trial. At the end of the trial, a 1-d force-feeding experiment was conducted using the aforementioned FF and experimental diets. Samples were collected for the analysis of postprandial kinetics of aminoacidemia, mechanistic target of rapamycin (mTOR) signaling activities, protein deposition, as well as the mRNA expression levels of key metabolic checkpoints at consecutive time points after feeding. Results Increased FF and leucine supplementation significantly enhanced fish growth by 7.68% ± 0.53% (means ±SD) and 7.89% ± 1.25%, respectively, and protein retention by 4.01% ± 0.59% and 4.44% ± 1.63%, respectively, in feeding trial experiments. The durations of postprandial aminoacidemia and mTOR activation were extended by increased FF, whereas leucine supplementation enhanced mTOR signaling without influencing the postprandial free amino acids kinetics. Increased FF and leucine supplementation enhanced muscle protein deposition 21.6% ± 6.85% and 22.3% ± 1.52%, respectively, in a 24-h postfeeding period. Conclusions We provided comprehensive characterization of the postprandial kinetics of nutrient sensing and metabolic responses under different feeding regimens and leucine supplementation in turbot. Fine-tuning of postprandial kinetics could provide a new direction for better dietary utilization and animal performances in aquaculture. Article in Journal/Newspaper Scophthalmus maximus Turbot Oxford University Press (via Crossref) The Journal of Nutrition |
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Oxford University Press (via Crossref) |
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croxfordunivpr |
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English |
topic |
Nutrition and Dietetics Medicine (miscellaneous) |
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Nutrition and Dietetics Medicine (miscellaneous) Wang, Ning Zhang, Xuemin Liu, Chengdong Wang, Xuan Zhou, Huihui Mai, Kangsen He, Gen Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
topic_facet |
Nutrition and Dietetics Medicine (miscellaneous) |
description |
ABSTRACT Background Feeding-induced cell signaling and metabolic responses affect utilization of dietary nutrients but are rarely taken advantage of to improve animal nutrition. Objectives We hypothesized that by modulating postprandial kinetics and signaling, improved dietary utilization and growth performance could be achieved in animals. Methods Juvenile turbot (Scophthalmus maximus L.) with an initial mean ± SD weight of 10.1 ± 0.01 g were used. Two feeding frequencies (FFs), either 1 or 3 meals/d at a fixed 2.4% daily body weight ration, and 2 diets that were or were not supplemented with 1% crystalline leucine (Leu), were used in the 10-wk feeding trial. At the end of the trial, a 1-d force-feeding experiment was conducted using the aforementioned FF and experimental diets. Samples were collected for the analysis of postprandial kinetics of aminoacidemia, mechanistic target of rapamycin (mTOR) signaling activities, protein deposition, as well as the mRNA expression levels of key metabolic checkpoints at consecutive time points after feeding. Results Increased FF and leucine supplementation significantly enhanced fish growth by 7.68% ± 0.53% (means ±SD) and 7.89% ± 1.25%, respectively, and protein retention by 4.01% ± 0.59% and 4.44% ± 1.63%, respectively, in feeding trial experiments. The durations of postprandial aminoacidemia and mTOR activation were extended by increased FF, whereas leucine supplementation enhanced mTOR signaling without influencing the postprandial free amino acids kinetics. Increased FF and leucine supplementation enhanced muscle protein deposition 21.6% ± 6.85% and 22.3% ± 1.52%, respectively, in a 24-h postfeeding period. Conclusions We provided comprehensive characterization of the postprandial kinetics of nutrient sensing and metabolic responses under different feeding regimens and leucine supplementation in turbot. Fine-tuning of postprandial kinetics could provide a new direction for better dietary utilization and animal performances in aquaculture. |
author2 |
National Key Research and Development Program of China National Natural Science Foundation of China National Laboratory for Marine Science and Technology Key R&D Program in Shandong Province China Agriculture Research System |
format |
Article in Journal/Newspaper |
author |
Wang, Ning Zhang, Xuemin Liu, Chengdong Wang, Xuan Zhou, Huihui Mai, Kangsen He, Gen |
author_facet |
Wang, Ning Zhang, Xuemin Liu, Chengdong Wang, Xuan Zhou, Huihui Mai, Kangsen He, Gen |
author_sort |
Wang, Ning |
title |
Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
title_short |
Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
title_full |
Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
title_fullStr |
Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
title_full_unstemmed |
Fine-Tuning of Postprandial Responses via Feeding Frequency and Leucine Supplementation Affects Dietary Performance in Turbot ( Scophthalmus maximus L.) |
title_sort |
fine-tuning of postprandial responses via feeding frequency and leucine supplementation affects dietary performance in turbot ( scophthalmus maximus l.) |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/jn/nxab221 http://academic.oup.com/jn/article-pdf/151/10/2957/40494640/nxab221.pdf |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
The Journal of Nutrition volume 151, issue 10, page 2957-2966 ISSN 0022-3166 1541-6100 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jn/nxab221 |
container_title |
The Journal of Nutrition |
_version_ |
1766188967652229120 |