Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages
Abstract Among the three main divergent lineages of gray wolf (Canis lupus), the Holarctic lineage is the most widespread and best studied, particularly in North America and Europe. Less is known about Tibetan (also called Himalayan) and Indian wolf lineages in southern Asia, especially in areas sur...
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Oxford University Press (OUP)
2023
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Online Access: | http://dx.doi.org/10.1093/jhered/esad066 https://academic.oup.com/jhered/advance-article-pdf/doi/10.1093/jhered/esad066/53596351/esad066.pdf https://academic.oup.com/jhered/article-pdf/115/4/339/58498687/esad066.pdf |
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croxfordunivpr:10.1093/jhered/esad066 2024-09-15T18:01:17+00:00 Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages Hennelly, Lauren M Sarwar, Ghulam Fatima, Hira Werhahn, Geraldine Abbas, Fakhar I Khan, Abdul M Mahmood, Tariq Kachel, Shannon Kubanychbekov, Zairbek Waseem, Muhammad T Zahra Naqvi, Rubab Hamid, Abdul Abbas, Yasir Aisha, Hamera Waseem, Muhammad Farooq, Muhammad Sacks, Benjamin N Shapiro, Beth UK Wolf Conservation Trust University of California Oxford-Lady Margaret Hall-Natural Motion Graduate Scholarship National Science Foundation 2023 http://dx.doi.org/10.1093/jhered/esad066 https://academic.oup.com/jhered/advance-article-pdf/doi/10.1093/jhered/esad066/53596351/esad066.pdf https://academic.oup.com/jhered/article-pdf/115/4/339/58498687/esad066.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights Journal of Heredity volume 115, issue 4, page 339-348 ISSN 0022-1503 1465-7333 journal-article 2023 croxfordunivpr https://doi.org/10.1093/jhered/esad066 2024-07-15T04:22:52Z Abstract Among the three main divergent lineages of gray wolf (Canis lupus), the Holarctic lineage is the most widespread and best studied, particularly in North America and Europe. Less is known about Tibetan (also called Himalayan) and Indian wolf lineages in southern Asia, especially in areas surrounding Pakistan where all three lineages are thought to meet. Given the endangered status of the Indian wolf in neighboring India and unclear southwestern boundary of the Tibetan wolf range, we conducted mitochondrial and genome-wide sequencing of wolves from Pakistan and Kyrgyzstan. Sequences of the mitochondrial D-loop region of 81 wolves from Pakistan indicated contact zones between Holarctic and Indian lineages across the northern and western mountains of Pakistan. Reduced-representation genome sequencing of eight wolves indicated an east-to-west cline of Indian to Holarctic ancestry, consistent with a contact zone between these two lineages in Pakistan. The western boundary of the Tibetan lineage corresponded to the Ladakh region of India’s Himalayas with a narrow zone of admixture spanning this boundary from the Karakoram Mountains of northern Pakistan into Ladakh, India. Our results highlight the conservation significance of Pakistan’s wolf populations, especially the remaining populations in Sindh and Southern Punjab that represent the highly endangered Indian lineage. Article in Journal/Newspaper Canis lupus gray wolf Oxford University Press Journal of Heredity |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Among the three main divergent lineages of gray wolf (Canis lupus), the Holarctic lineage is the most widespread and best studied, particularly in North America and Europe. Less is known about Tibetan (also called Himalayan) and Indian wolf lineages in southern Asia, especially in areas surrounding Pakistan where all three lineages are thought to meet. Given the endangered status of the Indian wolf in neighboring India and unclear southwestern boundary of the Tibetan wolf range, we conducted mitochondrial and genome-wide sequencing of wolves from Pakistan and Kyrgyzstan. Sequences of the mitochondrial D-loop region of 81 wolves from Pakistan indicated contact zones between Holarctic and Indian lineages across the northern and western mountains of Pakistan. Reduced-representation genome sequencing of eight wolves indicated an east-to-west cline of Indian to Holarctic ancestry, consistent with a contact zone between these two lineages in Pakistan. The western boundary of the Tibetan lineage corresponded to the Ladakh region of India’s Himalayas with a narrow zone of admixture spanning this boundary from the Karakoram Mountains of northern Pakistan into Ladakh, India. Our results highlight the conservation significance of Pakistan’s wolf populations, especially the remaining populations in Sindh and Southern Punjab that represent the highly endangered Indian lineage. |
author2 |
Shapiro, Beth UK Wolf Conservation Trust University of California Oxford-Lady Margaret Hall-Natural Motion Graduate Scholarship National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Hennelly, Lauren M Sarwar, Ghulam Fatima, Hira Werhahn, Geraldine Abbas, Fakhar I Khan, Abdul M Mahmood, Tariq Kachel, Shannon Kubanychbekov, Zairbek Waseem, Muhammad T Zahra Naqvi, Rubab Hamid, Abdul Abbas, Yasir Aisha, Hamera Waseem, Muhammad Farooq, Muhammad Sacks, Benjamin N |
spellingShingle |
Hennelly, Lauren M Sarwar, Ghulam Fatima, Hira Werhahn, Geraldine Abbas, Fakhar I Khan, Abdul M Mahmood, Tariq Kachel, Shannon Kubanychbekov, Zairbek Waseem, Muhammad T Zahra Naqvi, Rubab Hamid, Abdul Abbas, Yasir Aisha, Hamera Waseem, Muhammad Farooq, Muhammad Sacks, Benjamin N Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
author_facet |
Hennelly, Lauren M Sarwar, Ghulam Fatima, Hira Werhahn, Geraldine Abbas, Fakhar I Khan, Abdul M Mahmood, Tariq Kachel, Shannon Kubanychbekov, Zairbek Waseem, Muhammad T Zahra Naqvi, Rubab Hamid, Abdul Abbas, Yasir Aisha, Hamera Waseem, Muhammad Farooq, Muhammad Sacks, Benjamin N |
author_sort |
Hennelly, Lauren M |
title |
Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
title_short |
Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
title_full |
Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
title_fullStr |
Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
title_full_unstemmed |
Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages |
title_sort |
genomic analysis of wolves from pakistan clarifies boundaries among three divergent wolf lineages |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/jhered/esad066 https://academic.oup.com/jhered/advance-article-pdf/doi/10.1093/jhered/esad066/53596351/esad066.pdf https://academic.oup.com/jhered/article-pdf/115/4/339/58498687/esad066.pdf |
genre |
Canis lupus gray wolf |
genre_facet |
Canis lupus gray wolf |
op_source |
Journal of Heredity volume 115, issue 4, page 339-348 ISSN 0022-1503 1465-7333 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1093/jhered/esad066 |
container_title |
Journal of Heredity |
_version_ |
1810438455568629760 |