A Unique Mitochondrial Gene Block Inversion in Antarctic Trematomin Fishes: A Cautionary Tale

• Published in: Journal of Heredity
• Main Authors: Patel, Selina, Evans, Clive W, Stuckey, Alex, Matzke, Nicholas J, Millar, Craig D
• Other Authors: Baker, C Scott, University of Auckland, Allan Wilson Centre for Molecular Ecology and Evolution
• Format: Article in Journal/Newspaper
• Language: English
• Published: Oxford University Press (OUP) 2022
• Subjects: Antarc*; Antarctic; Antarctic silverfish; Antarctica
• Online Access: http://dx.doi.org/10.1093/jhered/esac028; https://academic.oup.com/jhered/advance-article-pdf/doi/10.1093/jhered/esac028/44318127/esac028.pdf; https://academic.oup.com/jhered/article-pdf/113/4/414/45050964/esac028.pdf

Abstract Many Antarctic notothenioid fishes have major rearrangements in their mitochondrial (mt) genomes. Here, we report the complete mt genomes of 3 trematomin notothenioids: the bald notothen (Trematomus (Pagothenia) borchgrevinki), the spotted notothen (T. nicolai), and the emerald notothen (T. bernacchii). The 3 mt genomes were sequenced using next-generation Illumina technology, and the assemblies verified by Sanger sequencing. When compared with the canonical mt gene order of the Antarctic silverfish (Pleuragramma antarctica), we found a large gene inversion in the 3 trematomin mt genomes that included tRNAIle, ND1, tRNALeu2, 16S, tRNAVal, 12S, tRNAPhe, and the control region. The trematomin mt genomes contained 3 intergenic spacers, which are thought to be the remnants of previous gene and control region duplications. All control regions included the characteristic conserved regulatory sequence motifs. Although short-read next-generation DNA sequencing technology has allowed the rapid and cost-effective sequencing of a large number of complete mt genomes, it is essential in all cases to verify the assembly in order to prevent the publication and use of erroneous data.