Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae)
ABSTRACT Increased anthropogenic input of carbon dioxide into the atmosphere has caused widespread patterns of ocean acidification (OA) and increased the frequency of extreme warming events. We explored the sublethal effects of OA on the hemolymph chemistry and physiological response to acute therma...
Published in: | Journal of Crustacean Biology |
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Oxford University Press (OUP)
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Online Access: | http://dx.doi.org/10.1093/jcbiol/ruz015 http://academic.oup.com/jcb/article-pdf/39/4/468/28993532/ruz015.pdf |
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croxfordunivpr:10.1093/jcbiol/ruz015 2024-09-15T18:27:59+00:00 Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) Harrington, Amalia M Hamlin, Heather J National Science Foundation United States Department of Agriculture National Institute of Food and Agriculture 2019 http://dx.doi.org/10.1093/jcbiol/ruz015 http://academic.oup.com/jcb/article-pdf/39/4/468/28993532/ruz015.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of Crustacean Biology volume 39, issue 4, page 468-476 ISSN 0278-0372 1937-240X journal-article 2019 croxfordunivpr https://doi.org/10.1093/jcbiol/ruz015 2024-07-29T04:21:33Z ABSTRACT Increased anthropogenic input of carbon dioxide into the atmosphere has caused widespread patterns of ocean acidification (OA) and increased the frequency of extreme warming events. We explored the sublethal effects of OA on the hemolymph chemistry and physiological response to acute thermal stress in the American lobster (Homarus americanus H. Milne Edwards, 1837). We exposed subadult lobsters to current or predicted end-century pH conditions (8.0 and 7.6, respectively) for 60 days. Following exposure, we assessed hemolymph L-lactate and calcium concentrations (as indicators of oxygen carrying capacity), ecdysterone concentrations, total protein content, and total hemocyte counts (THCs) as an indicator of immune response. We also assessed cardiac performance in the context of an acute warming event using impedance pneumography. Calcium, total protein, and ecdysterone concentrations were not significantly altered (P ≥ 0.10) by OA exposure. Control lobsters, however, had significantly higher levels of L-lactate concentrations compared to acidified lobsters, suggesting reduced oxygen carrying capacity under OA. THCs were also 61% higher in control versus acidified lobsters, suggesting immunosuppression under chronic OA. Lobsters exposed to acidified conditions exhibited reduced cardiac performance under acute warming as indicated by significantly lower (P = 0.040) Arrhenius Break Temperatures compared to control lobsters. These results suggest that although some physiological endpoints of American lobster are not impacted by OA, the stress of OA will likely be compounded by acute heat shock and may present additional physiological challenges for this species in the face of future change. Article in Journal/Newspaper Ocean acidification Oxford University Press Journal of Crustacean Biology 39 4 468 476 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
ABSTRACT Increased anthropogenic input of carbon dioxide into the atmosphere has caused widespread patterns of ocean acidification (OA) and increased the frequency of extreme warming events. We explored the sublethal effects of OA on the hemolymph chemistry and physiological response to acute thermal stress in the American lobster (Homarus americanus H. Milne Edwards, 1837). We exposed subadult lobsters to current or predicted end-century pH conditions (8.0 and 7.6, respectively) for 60 days. Following exposure, we assessed hemolymph L-lactate and calcium concentrations (as indicators of oxygen carrying capacity), ecdysterone concentrations, total protein content, and total hemocyte counts (THCs) as an indicator of immune response. We also assessed cardiac performance in the context of an acute warming event using impedance pneumography. Calcium, total protein, and ecdysterone concentrations were not significantly altered (P ≥ 0.10) by OA exposure. Control lobsters, however, had significantly higher levels of L-lactate concentrations compared to acidified lobsters, suggesting reduced oxygen carrying capacity under OA. THCs were also 61% higher in control versus acidified lobsters, suggesting immunosuppression under chronic OA. Lobsters exposed to acidified conditions exhibited reduced cardiac performance under acute warming as indicated by significantly lower (P = 0.040) Arrhenius Break Temperatures compared to control lobsters. These results suggest that although some physiological endpoints of American lobster are not impacted by OA, the stress of OA will likely be compounded by acute heat shock and may present additional physiological challenges for this species in the face of future change. |
author2 |
National Science Foundation United States Department of Agriculture National Institute of Food and Agriculture |
format |
Article in Journal/Newspaper |
author |
Harrington, Amalia M Hamlin, Heather J |
spellingShingle |
Harrington, Amalia M Hamlin, Heather J Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
author_facet |
Harrington, Amalia M Hamlin, Heather J |
author_sort |
Harrington, Amalia M |
title |
Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
title_short |
Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
title_full |
Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
title_fullStr |
Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
title_full_unstemmed |
Ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult American lobsters Homarus americanus H. Milne Edwards, 1837 (Decapoda: Malcostraca: Nephropidae) |
title_sort |
ocean acidification alters thermal cardiac performance, hemocyte abundance, and hemolymph chemistry in subadult american lobsters homarus americanus h. milne edwards, 1837 (decapoda: malcostraca: nephropidae) |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1093/jcbiol/ruz015 http://academic.oup.com/jcb/article-pdf/39/4/468/28993532/ruz015.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Journal of Crustacean Biology volume 39, issue 4, page 468-476 ISSN 0278-0372 1937-240X |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jcbiol/ruz015 |
container_title |
Journal of Crustacean Biology |
container_volume |
39 |
container_issue |
4 |
container_start_page |
468 |
op_container_end_page |
476 |
_version_ |
1810469278726488064 |