260 Identification of Candidate Gut Bacterial Species from Atlantic Salmon ( Salmo salar ) That Can Metabolize Ingredients Derived from Bioprocessed Soybean Meal

• Published in: Journal of Animal Science
• Main Authors: Fowler, Emily, St-Pierre, Benoit, Gibbons, William, Kerr, Phil, Brown, Michael
• Format: Article in Journal/Newspaper
• Language: English
• Published: Oxford University Press (OUP) 2023
• Subjects: Genetics; Animal Science and Zoology; General Medicine; Food Science; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1093/jas/skad281.222; https://academic.oup.com/jas/article-pdf/101/Supplement_3/182/52957603/skad281.222.pdf

Abstract In the aquaculture industry, plant-based protein sources have emerged as an economically and environmentally sustainable replacement for traditional animal protein sources. Yet, due to decreased digestibility, the presence of anti-nutritional factors, and an increased risk of inflammation of the digestive system, the inclusions of plant-based protein ingredients must be limited in the diets of carnivorous fishes. To gain further insight on the adaptation of the fish digestive tract to plant-based protein ingredients, slurry from cultured Atlantic Salmon (Salmo salar) was used as a proxy in an in vitro model system to identify candidate gut bacterial species from fish that can metabolize bioprocessed soybean meal. Triplicate slurry cultures were supplemented with one of each of the following ingredients: fishmeal (FM – control#1), soybean meal (SBM-control#2), MEPro (bioprocessed soybean meal product), as well as three ingredients derived from bioprocessed soybean meal (SPC1 - soy protein isolate #1; SPC2 - soy protein isolate #2; SPC3 - soy protein isolate #3). Data were generated by Illumina MiSeq 2X300 sequencing of PCR-generated amplicons targeting the V1-V3 regions of the 16S rRNA gene. A comparative analysis of the most highly represented Operational Taxonomic Units (OTU) using the non-parametric Kruskal-Wallis sum-rank test and Wilcoxon pairwise test identified candidate bacterial species that were enriched in cultures supplemented with specific bioprocessed soybean meal-derived ingredients compared with non-supplemented cultures (negative control). These results indicate that certain species may utilize these ingredients. Notably, OTUs Ss_1-37437 and Ss_1-13316 were found in the greatest abundance in cultures supplemented with SPC1 (31.4 - 49.7%) and SPC2 (7.2 - 17.4%), respectively. Interestingly, both OTUs were likely to represent novel bacterial species, based on their limited nucleotide sequence identity to their corresponding valid relative: Ss_1-37437: Prevotella paludivivens (96.58%); ...