PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding
Abstract Arctic and northern latitudes are rarely used in animal husbandry, meanwhile, wild species of animals that are best adapted to the harsh climate successfully live in these territories. The aim of the study was to obtain new breeding forms in sheep using assisted reproductive technologies. D...
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Online Access: | http://dx.doi.org/10.1093/jas/skab235.480 http://academic.oup.com/jas/article-pdf/99/Supplement_3/262/40529466/skab235.480.pdf |
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croxfordunivpr:10.1093/jas/skab235.480 2023-05-15T15:13:58+02:00 PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding Vinokurov, Nikolaj Vladimirov, Leonid Machakhtyrov, Grigoriy Machakhtyrova, Varvara Sleptsov, Evgeniy 2021 http://dx.doi.org/10.1093/jas/skab235.480 http://academic.oup.com/jas/article-pdf/99/Supplement_3/262/40529466/skab235.480.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of Animal Science volume 99, issue Supplement_3, page 262-263 ISSN 0021-8812 1525-3163 Genetics Animal Science and Zoology General Medicine Food Science journal-article 2021 croxfordunivpr https://doi.org/10.1093/jas/skab235.480 2022-04-15T06:19:02Z Abstract Arctic and northern latitudes are rarely used in animal husbandry, meanwhile, wild species of animals that are best adapted to the harsh climate successfully live in these territories. The aim of the study was to obtain new breeding forms in sheep using assisted reproductive technologies. Domestic sheep of the Buryat Coarsewool and sperm from 4 Siberian bighorn sheep of the Yakut population (Ovis nivicola lydekkeri) were used. Methods of postmortal removal of viable semen from epididymal aspirate and also the composition of a newly created extender for freezing epididymal semen have been developed. Thirteen sheep were inseminated using laparotomy. Sheep in estrus were identified by a test ram. The viability of the semen is influenced by the time interval from the moment of death to the removal of the semen from the epididymal aspirate. The temperature of the environment in which the testicles were kept before the semen removal also affects the final quality of the sperm. Thus, the highest viability of epididymal semen was observed at t = + 4°C when removed within 2 hours after the ram` death. As a result of research, the optimal composition of the extender was developed from test solutions of different compositions. The viability of spermatozoa for the extender was 25–30% higher than in others. Since the fertilizing properties of epididymal semen are lower than that of ejaculated semen, we used the intratubal insemination method. As a result, 8 hybrid F1 offsprings (1/2 domestic sheep x 1/2 wild bighorn sheep) were obtained, which can be kept year-round in winter pastures in the conditions of extremely low temperatures. Article in Journal/Newspaper Arctic Oxford University Press (via Crossref) Arctic Journal of Animal Science 99 Supplement_3 262 263 |
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Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Genetics Animal Science and Zoology General Medicine Food Science |
spellingShingle |
Genetics Animal Science and Zoology General Medicine Food Science Vinokurov, Nikolaj Vladimirov, Leonid Machakhtyrov, Grigoriy Machakhtyrova, Varvara Sleptsov, Evgeniy PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
topic_facet |
Genetics Animal Science and Zoology General Medicine Food Science |
description |
Abstract Arctic and northern latitudes are rarely used in animal husbandry, meanwhile, wild species of animals that are best adapted to the harsh climate successfully live in these territories. The aim of the study was to obtain new breeding forms in sheep using assisted reproductive technologies. Domestic sheep of the Buryat Coarsewool and sperm from 4 Siberian bighorn sheep of the Yakut population (Ovis nivicola lydekkeri) were used. Methods of postmortal removal of viable semen from epididymal aspirate and also the composition of a newly created extender for freezing epididymal semen have been developed. Thirteen sheep were inseminated using laparotomy. Sheep in estrus were identified by a test ram. The viability of the semen is influenced by the time interval from the moment of death to the removal of the semen from the epididymal aspirate. The temperature of the environment in which the testicles were kept before the semen removal also affects the final quality of the sperm. Thus, the highest viability of epididymal semen was observed at t = + 4°C when removed within 2 hours after the ram` death. As a result of research, the optimal composition of the extender was developed from test solutions of different compositions. The viability of spermatozoa for the extender was 25–30% higher than in others. Since the fertilizing properties of epididymal semen are lower than that of ejaculated semen, we used the intratubal insemination method. As a result, 8 hybrid F1 offsprings (1/2 domestic sheep x 1/2 wild bighorn sheep) were obtained, which can be kept year-round in winter pastures in the conditions of extremely low temperatures. |
format |
Article in Journal/Newspaper |
author |
Vinokurov, Nikolaj Vladimirov, Leonid Machakhtyrov, Grigoriy Machakhtyrova, Varvara Sleptsov, Evgeniy |
author_facet |
Vinokurov, Nikolaj Vladimirov, Leonid Machakhtyrov, Grigoriy Machakhtyrova, Varvara Sleptsov, Evgeniy |
author_sort |
Vinokurov, Nikolaj |
title |
PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
title_short |
PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
title_full |
PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
title_fullStr |
PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
title_full_unstemmed |
PSXV-11 Using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
title_sort |
psxv-11 using assisted reproductive technologies for obtaining new breeding forms by hybridization in sheep breeding |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/jas/skab235.480 http://academic.oup.com/jas/article-pdf/99/Supplement_3/262/40529466/skab235.480.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Animal Science volume 99, issue Supplement_3, page 262-263 ISSN 0021-8812 1525-3163 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jas/skab235.480 |
container_title |
Journal of Animal Science |
container_volume |
99 |
container_issue |
Supplement_3 |
container_start_page |
262 |
op_container_end_page |
263 |
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1766344481083228160 |