PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia
Abstract Domestic reindeer in Russia are a valuable resource of vital importance to the physical and cultural survival of the Northern indigenous minority. During the last decades, the mitochondrial (mt) genetic markers have been widely used as a molecular tool to investigate genetic structure and d...
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2021
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Online Access: | http://dx.doi.org/10.1093/jas/skab235.462 http://academic.oup.com/jas/article-pdf/99/Supplement_3/253/40528756/skab235.462.pdf |
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croxfordunivpr:10.1093/jas/skab235.462 2023-05-15T15:54:47+02:00 PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia Kharzinova, Veronika R Dotsev, Arsen V Bardukov, Nikolay V Deniskova, Tatiana E Upadhyay, Maulik Krebs, Stefan Kunz, Elisabeth Medugorac, Ivica Zinovieva, Natalia A 2021 http://dx.doi.org/10.1093/jas/skab235.462 http://academic.oup.com/jas/article-pdf/99/Supplement_3/253/40528756/skab235.462.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of Animal Science volume 99, issue Supplement_3, page 253-253 ISSN 0021-8812 1525-3163 Genetics Animal Science and Zoology General Medicine Food Science journal-article 2021 croxfordunivpr https://doi.org/10.1093/jas/skab235.462 2022-04-15T06:19:11Z Abstract Domestic reindeer in Russia are a valuable resource of vital importance to the physical and cultural survival of the Northern indigenous minority. During the last decades, the mitochondrial (mt) genetic markers have been widely used as a molecular tool to investigate genetic structure and diversity of livestock species. Here we aimed at the assessing the mtDNA diversity of the domestic reindeer inhabiting the area from the Kola Peninsula in the west to the Chukotka region in the east. A complete cytochrome b (cytb) sequences (1,140 bp) from representatives of six populations, including Nenets (NEN, n = 16), Evenk (EVK, n = 12), Even (EVN, n = 6), Chukotka (CHU, n = 6), Chukotka-Khargin (CHUKH, n = 6) and Tuva (TUVA, n = 6) were obtained. Sequences’ alignment was conducted using MUSCLE algorithm in R package msa. In total, 34 haplotypes were identified. Median-joining network, constructed in PopART 1.7, revealed three major groups of haplotypes: the first one joined the samples of all the populations, the second one included NEN, EVN and CHUKH, and the third group was presented by the one sample of CHU. AMOVA, calculated in Arlequin 3.5.2.2, showed that only 9.58% of molecular variance could be explained by the differences between populations and 90.42% - within populations. Genetic diversity parameters calculated in DnaSP 6.12.03, demonstrated that average number of nucleotide differences (K) was highest in CHUKH (28.333) and EVN (27.409) and lowest in TUVA (4.533) and EVK (5.400). Nucleotide diversity (Pi) was 0.01238±0.00559, 0.00474±0.00091, 0.02404±0.00453, 0.01281±0.00464, 0.02485±0.00744, and 0.00398±0.00110 for NEN, EVK, EVN, CHU, CHUKH and TUVA, respectively. Our study demonstrated the lack of clear genetic structure of the studied reindeer populations in relation to cytb sequence. The level of genetic diversity was associated with census size and was lowest in the smallest Tuva population. This study was supported by RSF-21-16-00071 and Russian Ministry of Science and Higher Education-0445-2019-0024. Article in Journal/Newspaper Chukotka Evenk kola peninsula nenets Oxford University Press (via Crossref) Kola Peninsula Journal of Animal Science 99 Supplement_3 253 253 |
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Open Polar |
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Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Genetics Animal Science and Zoology General Medicine Food Science |
spellingShingle |
Genetics Animal Science and Zoology General Medicine Food Science Kharzinova, Veronika R Dotsev, Arsen V Bardukov, Nikolay V Deniskova, Tatiana E Upadhyay, Maulik Krebs, Stefan Kunz, Elisabeth Medugorac, Ivica Zinovieva, Natalia A PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
topic_facet |
Genetics Animal Science and Zoology General Medicine Food Science |
description |
Abstract Domestic reindeer in Russia are a valuable resource of vital importance to the physical and cultural survival of the Northern indigenous minority. During the last decades, the mitochondrial (mt) genetic markers have been widely used as a molecular tool to investigate genetic structure and diversity of livestock species. Here we aimed at the assessing the mtDNA diversity of the domestic reindeer inhabiting the area from the Kola Peninsula in the west to the Chukotka region in the east. A complete cytochrome b (cytb) sequences (1,140 bp) from representatives of six populations, including Nenets (NEN, n = 16), Evenk (EVK, n = 12), Even (EVN, n = 6), Chukotka (CHU, n = 6), Chukotka-Khargin (CHUKH, n = 6) and Tuva (TUVA, n = 6) were obtained. Sequences’ alignment was conducted using MUSCLE algorithm in R package msa. In total, 34 haplotypes were identified. Median-joining network, constructed in PopART 1.7, revealed three major groups of haplotypes: the first one joined the samples of all the populations, the second one included NEN, EVN and CHUKH, and the third group was presented by the one sample of CHU. AMOVA, calculated in Arlequin 3.5.2.2, showed that only 9.58% of molecular variance could be explained by the differences between populations and 90.42% - within populations. Genetic diversity parameters calculated in DnaSP 6.12.03, demonstrated that average number of nucleotide differences (K) was highest in CHUKH (28.333) and EVN (27.409) and lowest in TUVA (4.533) and EVK (5.400). Nucleotide diversity (Pi) was 0.01238±0.00559, 0.00474±0.00091, 0.02404±0.00453, 0.01281±0.00464, 0.02485±0.00744, and 0.00398±0.00110 for NEN, EVK, EVN, CHU, CHUKH and TUVA, respectively. Our study demonstrated the lack of clear genetic structure of the studied reindeer populations in relation to cytb sequence. The level of genetic diversity was associated with census size and was lowest in the smallest Tuva population. This study was supported by RSF-21-16-00071 and Russian Ministry of Science and Higher Education-0445-2019-0024. |
format |
Article in Journal/Newspaper |
author |
Kharzinova, Veronika R Dotsev, Arsen V Bardukov, Nikolay V Deniskova, Tatiana E Upadhyay, Maulik Krebs, Stefan Kunz, Elisabeth Medugorac, Ivica Zinovieva, Natalia A |
author_facet |
Kharzinova, Veronika R Dotsev, Arsen V Bardukov, Nikolay V Deniskova, Tatiana E Upadhyay, Maulik Krebs, Stefan Kunz, Elisabeth Medugorac, Ivica Zinovieva, Natalia A |
author_sort |
Kharzinova, Veronika R |
title |
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
title_short |
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
title_full |
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
title_fullStr |
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
title_full_unstemmed |
PSXII-8 Mitochondrial DNA diversity within domestic reindeer in Russia |
title_sort |
psxii-8 mitochondrial dna diversity within domestic reindeer in russia |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/jas/skab235.462 http://academic.oup.com/jas/article-pdf/99/Supplement_3/253/40528756/skab235.462.pdf |
geographic |
Kola Peninsula |
geographic_facet |
Kola Peninsula |
genre |
Chukotka Evenk kola peninsula nenets |
genre_facet |
Chukotka Evenk kola peninsula nenets |
op_source |
Journal of Animal Science volume 99, issue Supplement_3, page 253-253 ISSN 0021-8812 1525-3163 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jas/skab235.462 |
container_title |
Journal of Animal Science |
container_volume |
99 |
container_issue |
Supplement_3 |
container_start_page |
253 |
op_container_end_page |
253 |
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1766390028621053952 |