Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection
Abstract A liquid chromatographic (LC) method for determining residues of the antiparasitic drugs emamectin (EMA) and ivermectin (IVR) in fish tissues has been developed. EMA and IVR residues are extracted with acetonitrile and cleaned up on a C18 solid-phase extraction column. Extracts are derivati...
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Oxford University Press (OUP)
2001
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Online Access: | http://dx.doi.org/10.1093/jaoac/84.5.1358 http://academic.oup.com/jaoac/article-pdf/84/5/1358/32415653/jaoac1358.pdf |
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croxfordunivpr:10.1093/jaoac/84.5.1358 2023-12-31T10:04:44+01:00 Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection Riet, Jeffrey M van de Brothers, Natalie N Pearce, Johanna N Burns, B Garth 2001 http://dx.doi.org/10.1093/jaoac/84.5.1358 http://academic.oup.com/jaoac/article-pdf/84/5/1358/32415653/jaoac1358.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of AOAC INTERNATIONAL volume 84, issue 5, page 1358-1362 ISSN 1060-3271 1944-7922 Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry journal-article 2001 croxfordunivpr https://doi.org/10.1093/jaoac/84.5.1358 2023-12-06T09:04:50Z Abstract A liquid chromatographic (LC) method for determining residues of the antiparasitic drugs emamectin (EMA) and ivermectin (IVR) in fish tissues has been developed. EMA and IVR residues are extracted with acetonitrile and cleaned up on a C18 solid-phase extraction column. Extracts are derivatized with 1-methylimidazole and trifluoroacetic anhydride and the components are determined by LC on a C18 reversed-phase column with fluorescence detection (excitation: 365 nm, emission: 470 nm). The mobile phase is 94% acetonitrile–water run isocratically. Calibration curves were linear between 1 and 32 ng injected for both EMA and IVR. The limit of detection for both analytes was 0.5 ng/g, with a limit of quantitation of 1.5 ng/g. Recoveries of EMA and IVR added to salmon muscle averaged 96 ± 9% and 86 ± 6%, respectively, at levels between 5 and 80 ng/g. The percent relative standard deviation for the described method was less than 7% over the range of concentrations studied. The operational errors, interferences, and recoveries for fortified samples compare favorably with an established IVR method. The recommended method is simple, rapid, and specific for monitoring residues of EMA and IVR in Atlantic salmon muscle. Article in Journal/Newspaper Atlantic salmon Oxford University Press (via Crossref) Journal of AOAC INTERNATIONAL 84 5 1358 1362 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
spellingShingle |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry Riet, Jeffrey M van de Brothers, Natalie N Pearce, Johanna N Burns, B Garth Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
topic_facet |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
description |
Abstract A liquid chromatographic (LC) method for determining residues of the antiparasitic drugs emamectin (EMA) and ivermectin (IVR) in fish tissues has been developed. EMA and IVR residues are extracted with acetonitrile and cleaned up on a C18 solid-phase extraction column. Extracts are derivatized with 1-methylimidazole and trifluoroacetic anhydride and the components are determined by LC on a C18 reversed-phase column with fluorescence detection (excitation: 365 nm, emission: 470 nm). The mobile phase is 94% acetonitrile–water run isocratically. Calibration curves were linear between 1 and 32 ng injected for both EMA and IVR. The limit of detection for both analytes was 0.5 ng/g, with a limit of quantitation of 1.5 ng/g. Recoveries of EMA and IVR added to salmon muscle averaged 96 ± 9% and 86 ± 6%, respectively, at levels between 5 and 80 ng/g. The percent relative standard deviation for the described method was less than 7% over the range of concentrations studied. The operational errors, interferences, and recoveries for fortified samples compare favorably with an established IVR method. The recommended method is simple, rapid, and specific for monitoring residues of EMA and IVR in Atlantic salmon muscle. |
format |
Article in Journal/Newspaper |
author |
Riet, Jeffrey M van de Brothers, Natalie N Pearce, Johanna N Burns, B Garth |
author_facet |
Riet, Jeffrey M van de Brothers, Natalie N Pearce, Johanna N Burns, B Garth |
author_sort |
Riet, Jeffrey M van de |
title |
Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
title_short |
Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
title_full |
Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
title_fullStr |
Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
title_full_unstemmed |
Simultaneous Determination of Emamectin and Ivermectin Residues in Atlantic Salmon Muscle by Liquid Chromatography with Fluorescence Detection |
title_sort |
simultaneous determination of emamectin and ivermectin residues in atlantic salmon muscle by liquid chromatography with fluorescence detection |
publisher |
Oxford University Press (OUP) |
publishDate |
2001 |
url |
http://dx.doi.org/10.1093/jaoac/84.5.1358 http://academic.oup.com/jaoac/article-pdf/84/5/1358/32415653/jaoac1358.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of AOAC INTERNATIONAL volume 84, issue 5, page 1358-1362 ISSN 1060-3271 1944-7922 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jaoac/84.5.1358 |
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Journal of AOAC INTERNATIONAL |
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84 |
container_issue |
5 |
container_start_page |
1358 |
op_container_end_page |
1362 |
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1786836146603425792 |