Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish
Abstract The approved use of oxytetracycline (OTC) in U.S. aquaculture is limited to specific diseases in salmonids and channel catfish. OTC may also be effective in controlling diseases in other fish species important to public aquaculture, but before approved use of OTC can be augmented, an analyt...
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Oxford University Press (OUP)
1998
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Online Access: | http://dx.doi.org/10.1093/jaoac/81.4.702 http://academic.oup.com/jaoac/article-pdf/81/4/702/32460176/jaoac0702.pdf |
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croxfordunivpr:10.1093/jaoac/81.4.702 2023-12-31T10:04:49+01:00 Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish Meinertz, Jeffery R Stehly, Guy R Gingerich, William H 1998 http://dx.doi.org/10.1093/jaoac/81.4.702 http://academic.oup.com/jaoac/article-pdf/81/4/702/32460176/jaoac0702.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of AOAC INTERNATIONAL volume 81, issue 4, page 702-708 ISSN 1060-3271 1944-7922 Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry journal-article 1998 croxfordunivpr https://doi.org/10.1093/jaoac/81.4.702 2023-12-06T08:47:26Z Abstract The approved use of oxytetracycline (OTC) in U.S. aquaculture is limited to specific diseases in salmonids and channel catfish. OTC may also be effective in controlling diseases in other fish species important to public aquaculture, but before approved use of OTC can be augmented, an analytical method for determining OTC in fillet tissue from multiple species of fish will be required to support residue depletion studies. The objective of this study was to develop and validate a liquid chromatographic (LC) method that is accurate, precise, and sensitive for OTC in edible fillets from multiple species of fish. Homogenized fillet tissues from walleye, Atlantic salmon, striped bass, white sturgeon, rainbow trout, and channel catfish were fortified with OTC at nominal concentrations of 10, 20, 100,1000, and 5000 ng/g. In tissues fortified with OTC at 100,1000, and 5000 ng/g, mean recoveries ranged from 83 to 90%, and relative standard deviations (RSDs) ranged from 0.9 to 5.8%. In all other tissues, mean recoveries ranged from 59 to 98%, and RSDs ranged from 3.3 to 20%. Method quantitation limits ranged from 6 to 22 ng/g for the 6 species. The LC parameters produced easily in teg ratable OTC peaks without coelution of endogenous compounds. The method is accurate, precise, and sensitive for OTC in fillet tissue from 6 species of fish from 5 phylogenetically diverse groups. Article in Journal/Newspaper Atlantic salmon Oxford University Press (via Crossref) Journal of AOAC INTERNATIONAL 81 4 702 708 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
spellingShingle |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry Meinertz, Jeffery R Stehly, Guy R Gingerich, William H Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
topic_facet |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
description |
Abstract The approved use of oxytetracycline (OTC) in U.S. aquaculture is limited to specific diseases in salmonids and channel catfish. OTC may also be effective in controlling diseases in other fish species important to public aquaculture, but before approved use of OTC can be augmented, an analytical method for determining OTC in fillet tissue from multiple species of fish will be required to support residue depletion studies. The objective of this study was to develop and validate a liquid chromatographic (LC) method that is accurate, precise, and sensitive for OTC in edible fillets from multiple species of fish. Homogenized fillet tissues from walleye, Atlantic salmon, striped bass, white sturgeon, rainbow trout, and channel catfish were fortified with OTC at nominal concentrations of 10, 20, 100,1000, and 5000 ng/g. In tissues fortified with OTC at 100,1000, and 5000 ng/g, mean recoveries ranged from 83 to 90%, and relative standard deviations (RSDs) ranged from 0.9 to 5.8%. In all other tissues, mean recoveries ranged from 59 to 98%, and RSDs ranged from 3.3 to 20%. Method quantitation limits ranged from 6 to 22 ng/g for the 6 species. The LC parameters produced easily in teg ratable OTC peaks without coelution of endogenous compounds. The method is accurate, precise, and sensitive for OTC in fillet tissue from 6 species of fish from 5 phylogenetically diverse groups. |
format |
Article in Journal/Newspaper |
author |
Meinertz, Jeffery R Stehly, Guy R Gingerich, William H |
author_facet |
Meinertz, Jeffery R Stehly, Guy R Gingerich, William H |
author_sort |
Meinertz, Jeffery R |
title |
Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
title_short |
Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
title_full |
Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
title_fullStr |
Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
title_full_unstemmed |
Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish |
title_sort |
liquid chromatographic determination of oxytetracycline in edible fish fillets from six species of fish |
publisher |
Oxford University Press (OUP) |
publishDate |
1998 |
url |
http://dx.doi.org/10.1093/jaoac/81.4.702 http://academic.oup.com/jaoac/article-pdf/81/4/702/32460176/jaoac0702.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of AOAC INTERNATIONAL volume 81, issue 4, page 702-708 ISSN 1060-3271 1944-7922 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jaoac/81.4.702 |
container_title |
Journal of AOAC INTERNATIONAL |
container_volume |
81 |
container_issue |
4 |
container_start_page |
702 |
op_container_end_page |
708 |
_version_ |
1786836249041960960 |