Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection
abstract A liquid chromatographic method was developed for determination of ivermectin B1a (IVR) extracted from raw fortified and incurred Atlantic salmon muscle tissues. The method was also used to determine fortified doramectin (DOR) in Atlantic salmon. Tissue extract was applied to a C8 solid-pha...
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Oxford University Press (OUP)
1998
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Online Access: | http://dx.doi.org/10.1093/jaoac/81.3.549 http://academic.oup.com/jaoac/article-pdf/81/3/549/32459631/jaoac0549.pdf |
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croxfordunivpr:10.1093/jaoac/81.3.549 2023-12-31T10:04:45+01:00 Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection Rupp, Heidi S Turnipseed, Sherri B Walker, Calvin C Roybal, José E Long, Austin R 1998 http://dx.doi.org/10.1093/jaoac/81.3.549 http://academic.oup.com/jaoac/article-pdf/81/3/549/32459631/jaoac0549.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Journal of AOAC INTERNATIONAL volume 81, issue 3, page 549-553 ISSN 1060-3271 1944-7922 Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry journal-article 1998 croxfordunivpr https://doi.org/10.1093/jaoac/81.3.549 2023-12-06T08:40:56Z abstract A liquid chromatographic method was developed for determination of ivermectin B1a (IVR) extracted from raw fortified and incurred Atlantic salmon muscle tissues. The method was also used to determine fortified doramectin (DOR) in Atlantic salmon. Tissue extract was applied to a C8 solid-phase extraction (SPE) column, followed by a silica SPE column. Residues in the eluate were treated with trifluoroacetic anhydride and methylimidazole to dehydrate the IVR molecule and form an aromatic fluorescent moiety with a trifluoroacetic ester. This product was subsequently treated with ammonium acetate in methanol to cleave the ester and convert the functional group back to a stable alcohol form. The analytes were determined by fluorescence with excitation at 272 nm and emission at 465 nm. A Cis Hypersil column was used for analysis with a mobile phase of acetonitrile-water (90 + 10, v/v) and an oven temperature of 65°C. IVR and DOR were determined at 5 fortification levels (1, 5,10, 20, and 40 ppb). Intra-assay absolute recoveries ranged from 75 to 89% for IVR and from 73 to 85% for DOR. Relative standard deviations (RSDs) were <7% in all cases. The limit of detection (3 x baseline noise) was 0.25 ppb extracted from tissue. Incurred tissues had an average concentration of 32 ppb, with an RSD of 3%. Article in Journal/Newspaper Atlantic salmon Oxford University Press (via Crossref) Journal of AOAC INTERNATIONAL 81 3 549 553 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
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Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry Rupp, Heidi S Turnipseed, Sherri B Walker, Calvin C Roybal, José E Long, Austin R Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
topic_facet |
Pharmacology Agronomy and Crop Science Environmental Chemistry Food Science Analytical Chemistry |
description |
abstract A liquid chromatographic method was developed for determination of ivermectin B1a (IVR) extracted from raw fortified and incurred Atlantic salmon muscle tissues. The method was also used to determine fortified doramectin (DOR) in Atlantic salmon. Tissue extract was applied to a C8 solid-phase extraction (SPE) column, followed by a silica SPE column. Residues in the eluate were treated with trifluoroacetic anhydride and methylimidazole to dehydrate the IVR molecule and form an aromatic fluorescent moiety with a trifluoroacetic ester. This product was subsequently treated with ammonium acetate in methanol to cleave the ester and convert the functional group back to a stable alcohol form. The analytes were determined by fluorescence with excitation at 272 nm and emission at 465 nm. A Cis Hypersil column was used for analysis with a mobile phase of acetonitrile-water (90 + 10, v/v) and an oven temperature of 65°C. IVR and DOR were determined at 5 fortification levels (1, 5,10, 20, and 40 ppb). Intra-assay absolute recoveries ranged from 75 to 89% for IVR and from 73 to 85% for DOR. Relative standard deviations (RSDs) were <7% in all cases. The limit of detection (3 x baseline noise) was 0.25 ppb extracted from tissue. Incurred tissues had an average concentration of 32 ppb, with an RSD of 3%. |
format |
Article in Journal/Newspaper |
author |
Rupp, Heidi S Turnipseed, Sherri B Walker, Calvin C Roybal, José E Long, Austin R |
author_facet |
Rupp, Heidi S Turnipseed, Sherri B Walker, Calvin C Roybal, José E Long, Austin R |
author_sort |
Rupp, Heidi S |
title |
Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
title_short |
Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
title_full |
Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
title_fullStr |
Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
title_full_unstemmed |
Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection |
title_sort |
determination of ivermectin in salmon muscle tissue by liquid chromatography with fluorescence detection |
publisher |
Oxford University Press (OUP) |
publishDate |
1998 |
url |
http://dx.doi.org/10.1093/jaoac/81.3.549 http://academic.oup.com/jaoac/article-pdf/81/3/549/32459631/jaoac0549.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of AOAC INTERNATIONAL volume 81, issue 3, page 549-553 ISSN 1060-3271 1944-7922 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/jaoac/81.3.549 |
container_title |
Journal of AOAC INTERNATIONAL |
container_volume |
81 |
container_issue |
3 |
container_start_page |
549 |
op_container_end_page |
553 |
_version_ |
1786836164138762240 |