Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It all...
Published in: | Integrative Organismal Biology |
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Oxford University Press (OUP)
2023
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Online Access: | http://dx.doi.org/10.1093/iob/obad020 https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf |
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croxfordunivpr:10.1093/iob/obad020 2024-01-14T10:10:59+01:00 Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H National Science Foundation OVCR National Institutes of Health National Institute of General Medical Sciences 2023 http://dx.doi.org/10.1093/iob/obad020 https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Integrative Organismal Biology volume 5, issue 1 ISSN 2517-4843 Plant Science Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 2023 croxfordunivpr https://doi.org/10.1093/iob/obad020 2023-12-15T09:58:53Z Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes ... Article in Journal/Newspaper Subarctic Oxford University Press (via Crossref) Integrative Organismal Biology 5 1 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Plant Science Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Plant Science Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
topic_facet |
Plant Science Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
description |
Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes ... |
author2 |
National Science Foundation OVCR National Institutes of Health National Institute of General Medical Sciences |
format |
Article in Journal/Newspaper |
author |
Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H |
author_facet |
Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H |
author_sort |
Monell, K J |
title |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_short |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_full |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_fullStr |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_full_unstemmed |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_sort |
post-diapause dna replication during oogenesis in a capital-breeding copepod |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/iob/obad020 https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Integrative Organismal Biology volume 5, issue 1 ISSN 2517-4843 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/iob/obad020 |
container_title |
Integrative Organismal Biology |
container_volume |
5 |
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1 |
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1788065847214866432 |