Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod

Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It all...

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Published in:Integrative Organismal Biology
Main Authors: Monell, K J, Roncalli, V, Hopcroft, R R, Hartline, D K, Lenz, P H
Other Authors: National Science Foundation, OVCR, National Institutes of Health, National Institute of General Medical Sciences
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press (OUP) 2023
Subjects:
Online Access:http://dx.doi.org/10.1093/iob/obad020
https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf
https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf
id croxfordunivpr:10.1093/iob/obad020
record_format openpolar
spelling croxfordunivpr:10.1093/iob/obad020 2024-01-14T10:10:59+01:00 Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H National Science Foundation OVCR National Institutes of Health National Institute of General Medical Sciences 2023 http://dx.doi.org/10.1093/iob/obad020 https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Integrative Organismal Biology volume 5, issue 1 ISSN 2517-4843 Plant Science Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 2023 croxfordunivpr https://doi.org/10.1093/iob/obad020 2023-12-15T09:58:53Z Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes ... Article in Journal/Newspaper Subarctic Oxford University Press (via Crossref) Integrative Organismal Biology 5 1
institution Open Polar
collection Oxford University Press (via Crossref)
op_collection_id croxfordunivpr
language English
topic Plant Science
Animal Science and Zoology
Ecology, Evolution, Behavior and Systematics
spellingShingle Plant Science
Animal Science and Zoology
Ecology, Evolution, Behavior and Systematics
Monell, K J
Roncalli, V
Hopcroft, R R
Hartline, D K
Lenz, P H
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
topic_facet Plant Science
Animal Science and Zoology
Ecology, Evolution, Behavior and Systematics
description Synopsis In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes ...
author2 National Science Foundation
OVCR
National Institutes of Health
National Institute of General Medical Sciences
format Article in Journal/Newspaper
author Monell, K J
Roncalli, V
Hopcroft, R R
Hartline, D K
Lenz, P H
author_facet Monell, K J
Roncalli, V
Hopcroft, R R
Hartline, D K
Lenz, P H
author_sort Monell, K J
title Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
title_short Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
title_full Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
title_fullStr Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
title_full_unstemmed Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
title_sort post-diapause dna replication during oogenesis in a capital-breeding copepod
publisher Oxford University Press (OUP)
publishDate 2023
url http://dx.doi.org/10.1093/iob/obad020
https://academic.oup.com/iob/advance-article-pdf/doi/10.1093/iob/obad020/50574597/obad020.pdf
https://academic.oup.com/iob/article-pdf/5/1/obad020/50781101/obad020.pdf
genre Subarctic
genre_facet Subarctic
op_source Integrative Organismal Biology
volume 5, issue 1
ISSN 2517-4843
op_rights https://creativecommons.org/licenses/by/4.0/
op_doi https://doi.org/10.1093/iob/obad020
container_title Integrative Organismal Biology
container_volume 5
container_issue 1
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