1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada
Abstract Background Our community-driven projects address concerns of Canadian Arctic Indigenous communities about Helicobacter pylori (Hp) infection, responsible for elevated gastric cancer mortality in the region. Community research partners wished to learn whether bacterial characteristics determ...
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croxfordunivpr:10.1093/ije/dyab168.147 2024-09-15T18:26:39+00:00 1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada Cromarty, Taylor Quilty, Douglas Assi, Ali Girgis, Safwat van Zanten, Sander Veldhuyzen Goodman, Karen 2021 http://dx.doi.org/10.1093/ije/dyab168.147 http://academic.oup.com/ije/article-pdf/50/Supplement_1/dyab168.147/40211917/dyab168.147.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model International Journal of Epidemiology volume 50, issue Supplement_1 ISSN 0300-5771 1464-3685 journal-article 2021 croxfordunivpr https://doi.org/10.1093/ije/dyab168.147 2024-08-12T04:23:07Z Abstract Background Our community-driven projects address concerns of Canadian Arctic Indigenous communities about Helicobacter pylori (Hp) infection, responsible for elevated gastric cancer mortality in the region. Community research partners wished to learn whether bacterial characteristics determine severity of Hp-related disease in their communities. We aimed to describe gastric histopathology by cagA genotype of Hp isolated from residents of 7 Indigenous communities in the Northwest Territories and Yukon. Methods Participants underwent gastroscopy with 5-6 biopsies taken for histopathological assessment and 2 biopsies taken for tissue culture during 2008-2017. We used multiple PCR reactions and DNA sequence analysis to classify Hp genotypes as cagA+ or cagA-. A single pathologist used the updated Sydney classification system to grade severity of 5 gastric pathology outcomes: Hp density; chronic gastritis; active gastritis; atrophy; and intestinal metaplasia. We estimated prevalence of each outcome with 95% confidence intervals (CI) by gastric subsite and cagA status. Results Of 262 Hp isolates assessed, 142 (54%) were cagA+. Prevalence of moderate-high Hp density, severe chronic gastritis, moderate-severe active gastritis, atrophy, and metaplasia were (%[CI]): respectively, 78[70-85], 44[36-53], 65[56-72], 55[46-63], 25[18-33] in cagA+ participants and 61[52-70], 35[27-44], 31[23-40], 32[23-41], 8[4-15] in cagA- participants. cagA+ participants had higher prevalence of all outcomes in antrum and corpus. Conclusion Hp-infected Indigenous residents of Arctic Canada who harbored cagA-positive strains had higher prevalence of more severe gastric pathology than those with cagA-negative strains. Key messages Community-driven research answers questions posed by those who bear the disease burden. Article in Journal/Newspaper Northwest Territories Yukon Oxford University Press International Journal of Epidemiology 50 Supplement_1 |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Background Our community-driven projects address concerns of Canadian Arctic Indigenous communities about Helicobacter pylori (Hp) infection, responsible for elevated gastric cancer mortality in the region. Community research partners wished to learn whether bacterial characteristics determine severity of Hp-related disease in their communities. We aimed to describe gastric histopathology by cagA genotype of Hp isolated from residents of 7 Indigenous communities in the Northwest Territories and Yukon. Methods Participants underwent gastroscopy with 5-6 biopsies taken for histopathological assessment and 2 biopsies taken for tissue culture during 2008-2017. We used multiple PCR reactions and DNA sequence analysis to classify Hp genotypes as cagA+ or cagA-. A single pathologist used the updated Sydney classification system to grade severity of 5 gastric pathology outcomes: Hp density; chronic gastritis; active gastritis; atrophy; and intestinal metaplasia. We estimated prevalence of each outcome with 95% confidence intervals (CI) by gastric subsite and cagA status. Results Of 262 Hp isolates assessed, 142 (54%) were cagA+. Prevalence of moderate-high Hp density, severe chronic gastritis, moderate-severe active gastritis, atrophy, and metaplasia were (%[CI]): respectively, 78[70-85], 44[36-53], 65[56-72], 55[46-63], 25[18-33] in cagA+ participants and 61[52-70], 35[27-44], 31[23-40], 32[23-41], 8[4-15] in cagA- participants. cagA+ participants had higher prevalence of all outcomes in antrum and corpus. Conclusion Hp-infected Indigenous residents of Arctic Canada who harbored cagA-positive strains had higher prevalence of more severe gastric pathology than those with cagA-negative strains. Key messages Community-driven research answers questions posed by those who bear the disease burden. |
format |
Article in Journal/Newspaper |
author |
Cromarty, Taylor Quilty, Douglas Assi, Ali Girgis, Safwat van Zanten, Sander Veldhuyzen Goodman, Karen |
spellingShingle |
Cromarty, Taylor Quilty, Douglas Assi, Ali Girgis, Safwat van Zanten, Sander Veldhuyzen Goodman, Karen 1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
author_facet |
Cromarty, Taylor Quilty, Douglas Assi, Ali Girgis, Safwat van Zanten, Sander Veldhuyzen Goodman, Karen |
author_sort |
Cromarty, Taylor |
title |
1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
title_short |
1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
title_full |
1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
title_fullStr |
1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
title_full_unstemmed |
1447Gastric histopathology by Helicobacter pylori cagA status in Arctic Canada |
title_sort |
1447gastric histopathology by helicobacter pylori caga status in arctic canada |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/ije/dyab168.147 http://academic.oup.com/ije/article-pdf/50/Supplement_1/dyab168.147/40211917/dyab168.147.pdf |
genre |
Northwest Territories Yukon |
genre_facet |
Northwest Territories Yukon |
op_source |
International Journal of Epidemiology volume 50, issue Supplement_1 ISSN 0300-5771 1464-3685 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/ije/dyab168.147 |
container_title |
International Journal of Epidemiology |
container_volume |
50 |
container_issue |
Supplement_1 |
_version_ |
1810467170864332800 |