Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes
Abstract Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this tec...
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Oxford University Press (OUP)
2014
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Online Access: | http://dx.doi.org/10.1093/icesjms/fsu091 http://academic.oup.com/icesjms/article-pdf/71/9/2530/29147091/fsu091.pdf |
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croxfordunivpr:10.1093/icesjms/fsu091 2024-09-15T17:56:27+00:00 Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. 2014 http://dx.doi.org/10.1093/icesjms/fsu091 http://academic.oup.com/icesjms/article-pdf/71/9/2530/29147091/fsu091.pdf en eng Oxford University Press (OUP) ICES Journal of Marine Science volume 71, issue 9, page 2530-2538 ISSN 1054-3139 1095-9289 journal-article 2014 croxfordunivpr https://doi.org/10.1093/icesjms/fsu091 2024-08-05T04:34:13Z Abstract Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this technique. Osmotic induction improves incorporation and reduces immersion time for some chemical markers, but its effects on isotope incorporation into otoliths are unknown. Here, we tested the effects of osmotic induction over a range of different isotope concentrations and immersion times on relative mark success and strength for 26Mg:24Mg, 86Sr:88Sr and 137Ba:138Ba on Atlantic salmon (Salmo salar) larvae. 71% and 100% mark success were achieved after 1 h of immersion for 86Sr (75 µg L−1) and 137Ba (30 µg L−1) isotopes, respectively. Compared with conventional immersion, osmotic induction improved overall mark strength for 86Sr and 137Ba isotopes by 26–116%, although this effect was only observed after 12 h of immersion and predominately for 86Sr. The results demonstrate that osmotic induction reduces immersion times and the concentrations of isotope required to achieve successful marks. Osmotically induced isotope labels via larval immersion may prove a rapid and cost-effective way of batch marking fish larvae across a range of potential applications. Article in Journal/Newspaper Atlantic salmon Salmo salar Oxford University Press ICES Journal of Marine Science 71 9 2530 2538 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
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English |
description |
Abstract Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this technique. Osmotic induction improves incorporation and reduces immersion time for some chemical markers, but its effects on isotope incorporation into otoliths are unknown. Here, we tested the effects of osmotic induction over a range of different isotope concentrations and immersion times on relative mark success and strength for 26Mg:24Mg, 86Sr:88Sr and 137Ba:138Ba on Atlantic salmon (Salmo salar) larvae. 71% and 100% mark success were achieved after 1 h of immersion for 86Sr (75 µg L−1) and 137Ba (30 µg L−1) isotopes, respectively. Compared with conventional immersion, osmotic induction improved overall mark strength for 86Sr and 137Ba isotopes by 26–116%, although this effect was only observed after 12 h of immersion and predominately for 86Sr. The results demonstrate that osmotic induction reduces immersion times and the concentrations of isotope required to achieve successful marks. Osmotically induced isotope labels via larval immersion may prove a rapid and cost-effective way of batch marking fish larvae across a range of potential applications. |
format |
Article in Journal/Newspaper |
author |
de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. |
spellingShingle |
de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
author_facet |
de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. |
author_sort |
de Braux, Emmanuel |
title |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_short |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_full |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_fullStr |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_full_unstemmed |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_sort |
osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
publisher |
Oxford University Press (OUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1093/icesjms/fsu091 http://academic.oup.com/icesjms/article-pdf/71/9/2530/29147091/fsu091.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ICES Journal of Marine Science volume 71, issue 9, page 2530-2538 ISSN 1054-3139 1095-9289 |
op_doi |
https://doi.org/10.1093/icesjms/fsu091 |
container_title |
ICES Journal of Marine Science |
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71 |
container_issue |
9 |
container_start_page |
2530 |
op_container_end_page |
2538 |
_version_ |
1810432659199885312 |