eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment
Abstract Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We cond...
Published in: | ICES Journal of Marine Science |
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2023
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Online Access: | http://dx.doi.org/10.1093/icesjms/fsad083 https://academic.oup.com/icesjms/article-pdf/80/5/1529/50737377/fsad083.pdf |
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croxfordunivpr:10.1093/icesjms/fsad083 2024-09-15T18:26:22+00:00 eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment He, X Jeffery, N W Stanley, R R E Hamilton, L C Rubidge, E M Abbott, C L Grant, W Stewart Fisheries and Oceans Canada Genomics Research and Development Initiative 2023 http://dx.doi.org/10.1093/icesjms/fsad083 https://academic.oup.com/icesjms/article-pdf/80/5/1529/50737377/fsad083.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model ICES Journal of Marine Science volume 80, issue 5, page 1529-1538 ISSN 1054-3139 1095-9289 journal-article 2023 croxfordunivpr https://doi.org/10.1093/icesjms/fsad083 2024-09-03T04:12:49Z Abstract Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes. Article in Journal/Newspaper Northwest Atlantic Oxford University Press ICES Journal of Marine Science |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes. |
author2 |
Grant, W Stewart Fisheries and Oceans Canada Genomics Research and Development Initiative |
format |
Article in Journal/Newspaper |
author |
He, X Jeffery, N W Stanley, R R E Hamilton, L C Rubidge, E M Abbott, C L |
spellingShingle |
He, X Jeffery, N W Stanley, R R E Hamilton, L C Rubidge, E M Abbott, C L eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
author_facet |
He, X Jeffery, N W Stanley, R R E Hamilton, L C Rubidge, E M Abbott, C L |
author_sort |
He, X |
title |
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
title_short |
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
title_full |
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
title_fullStr |
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
title_full_unstemmed |
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
title_sort |
edna metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/icesjms/fsad083 https://academic.oup.com/icesjms/article-pdf/80/5/1529/50737377/fsad083.pdf |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
ICES Journal of Marine Science volume 80, issue 5, page 1529-1538 ISSN 1054-3139 1095-9289 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/icesjms/fsad083 |
container_title |
ICES Journal of Marine Science |
_version_ |
1810466852400267264 |