Environmental DNA metabarcoding describes biodiversity across marine gradients
Abstract In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targete...
Published in: | ICES Journal of Marine Science |
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Language: | English |
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Oxford University Press (OUP)
2023
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Online Access: | http://dx.doi.org/10.1093/icesjms/fsad017 https://academic.oup.com/icesjms/article-pdf/80/4/953/50384671/fsad017.pdf |
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croxfordunivpr:10.1093/icesjms/fsad017 2024-09-15T17:48:21+00:00 Environmental DNA metabarcoding describes biodiversity across marine gradients Adams, Clare I M Jeunen, Gert-Jan Cross, Hugh Taylor, Helen R Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J Urban, Lara Baltar, Federico Stat, Michael Bunce, Michael Knapp, Michael Miller-Saunders, Kristi University of Otago University of Otago Doctoral Scholarship 2023 http://dx.doi.org/10.1093/icesjms/fsad017 https://academic.oup.com/icesjms/article-pdf/80/4/953/50384671/fsad017.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ ICES Journal of Marine Science volume 80, issue 4, page 953-971 ISSN 1054-3139 1095-9289 journal-article 2023 croxfordunivpr https://doi.org/10.1093/icesjms/fsad017 2024-08-05T04:27:41Z Abstract In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targeted three molecular markers to determine multicellular metazoan communities from two timepoints across a long-standing transect in the Southern Hemisphere, the Munida Observational Time Series. We detected four community types across the successive water masses—neritic, sub-tropical, frontal, and sub-Antarctic—crossed by the transect, together with important community differences between the two sampling points. From indicator species analysis, we found diversity patterns were mostly driven by planktonic organisms. Mesopelagic communities differed from surface-water communities in the sub-Antarctic water mass, with at-depth communities dominated by single-cellular organisms. We evaluate the ability of eDNA to detect species-compositional changes across surface and depth gradients and lay the foundations for using this technique in multi-trophic environmental monitoring efforts across long time series. We observed community differences across time and space. More intensive sampling will be critical to fully capture diversity across marine gradients, but this multi-trophic method represents an invaluable opportunity to understand shifts in marine biota. Article in Journal/Newspaper Antarc* Antarctic Oxford University Press ICES Journal of Marine Science |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targeted three molecular markers to determine multicellular metazoan communities from two timepoints across a long-standing transect in the Southern Hemisphere, the Munida Observational Time Series. We detected four community types across the successive water masses—neritic, sub-tropical, frontal, and sub-Antarctic—crossed by the transect, together with important community differences between the two sampling points. From indicator species analysis, we found diversity patterns were mostly driven by planktonic organisms. Mesopelagic communities differed from surface-water communities in the sub-Antarctic water mass, with at-depth communities dominated by single-cellular organisms. We evaluate the ability of eDNA to detect species-compositional changes across surface and depth gradients and lay the foundations for using this technique in multi-trophic environmental monitoring efforts across long time series. We observed community differences across time and space. More intensive sampling will be critical to fully capture diversity across marine gradients, but this multi-trophic method represents an invaluable opportunity to understand shifts in marine biota. |
author2 |
Miller-Saunders, Kristi University of Otago University of Otago Doctoral Scholarship |
format |
Article in Journal/Newspaper |
author |
Adams, Clare I M Jeunen, Gert-Jan Cross, Hugh Taylor, Helen R Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J Urban, Lara Baltar, Federico Stat, Michael Bunce, Michael Knapp, Michael |
spellingShingle |
Adams, Clare I M Jeunen, Gert-Jan Cross, Hugh Taylor, Helen R Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J Urban, Lara Baltar, Federico Stat, Michael Bunce, Michael Knapp, Michael Environmental DNA metabarcoding describes biodiversity across marine gradients |
author_facet |
Adams, Clare I M Jeunen, Gert-Jan Cross, Hugh Taylor, Helen R Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J Urban, Lara Baltar, Federico Stat, Michael Bunce, Michael Knapp, Michael |
author_sort |
Adams, Clare I M |
title |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_short |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_full |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_fullStr |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_full_unstemmed |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_sort |
environmental dna metabarcoding describes biodiversity across marine gradients |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/icesjms/fsad017 https://academic.oup.com/icesjms/article-pdf/80/4/953/50384671/fsad017.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
ICES Journal of Marine Science volume 80, issue 4, page 953-971 ISSN 1054-3139 1095-9289 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/icesjms/fsad017 |
container_title |
ICES Journal of Marine Science |
_version_ |
1810289490928861184 |