Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing
Abstract Northern sand lance (Ammodytes dubius) are essential forage fish in most offshore, temperate-to-polar waters on the Northwest Atlantic shelf (NWA), but their population structure and genetic separation from the American sand lance (A. americanus) remain unresolved. We assembled a reference...
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Language: | English |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/icesjms/fsac217 https://academic.oup.com/icesjms/article-pdf/80/1/122/48860200/fsac217.pdf |
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croxfordunivpr:10.1093/icesjms/fsac217 2024-09-15T18:09:54+00:00 Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing Jones, Lucas F Lou, R Nicolas Murray, Christopher S Robert, Dominique Bourne, Christina M Bouchard, Caroline Kučka, Marek Chan, Y Frank Carlon, David B Wiley, David N Therkildsen, Nina O Baumann, Hannes Miller-Saunders, Kristi Bureau of Ocean Energy Management NSF OCE ERC UConn College of Liberal Arts and Sciences Department of Marine Sciences 2022 http://dx.doi.org/10.1093/icesjms/fsac217 https://academic.oup.com/icesjms/article-pdf/80/1/122/48860200/fsac217.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ ICES Journal of Marine Science volume 80, issue 1, page 122-132 ISSN 1054-3139 1095-9289 journal-article 2022 croxfordunivpr https://doi.org/10.1093/icesjms/fsac217 2024-07-29T04:22:09Z Abstract Northern sand lance (Ammodytes dubius) are essential forage fish in most offshore, temperate-to-polar waters on the Northwest Atlantic shelf (NWA), but their population structure and genetic separation from the American sand lance (A. americanus) remain unresolved. We assembled a reference genome for A. dubius (first in the Ammodytidae) and then used low-coverage whole genome sequencing on 262 specimens collected across the species distribution (Mid-Atlantic Bight to Greenland) to quantify genetic differentiation between geographic regions based on single nucleotide polymorphisms. We found strong separation between A. dubius from locations north and south of the Scotian Shelf, largely due to massive genetic differentiation spanning most of chromosomes 21 and 24. Genetic distance increased with geographic distance in the smaller southern cluster but not in the larger northern cluster, where genetic homogeneity appeared across large geographic distances (>103 km). The two genetic clusters coincide with a clear break in winter sea surface temperature, suggesting that differential offspring survival, rather than limited transport, causes a break in realized connectivity. Nuclear and mitochondrial DNA both clearly delineated A. dubius from A. americanus, thereby confirming a species boundary through spatial niche partitioning into inshore (A. americanus) and offshore (A. dubius) sand lance species on the NWA. Article in Journal/Newspaper Greenland Northwest Atlantic Oxford University Press ICES Journal of Marine Science |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Northern sand lance (Ammodytes dubius) are essential forage fish in most offshore, temperate-to-polar waters on the Northwest Atlantic shelf (NWA), but their population structure and genetic separation from the American sand lance (A. americanus) remain unresolved. We assembled a reference genome for A. dubius (first in the Ammodytidae) and then used low-coverage whole genome sequencing on 262 specimens collected across the species distribution (Mid-Atlantic Bight to Greenland) to quantify genetic differentiation between geographic regions based on single nucleotide polymorphisms. We found strong separation between A. dubius from locations north and south of the Scotian Shelf, largely due to massive genetic differentiation spanning most of chromosomes 21 and 24. Genetic distance increased with geographic distance in the smaller southern cluster but not in the larger northern cluster, where genetic homogeneity appeared across large geographic distances (>103 km). The two genetic clusters coincide with a clear break in winter sea surface temperature, suggesting that differential offspring survival, rather than limited transport, causes a break in realized connectivity. Nuclear and mitochondrial DNA both clearly delineated A. dubius from A. americanus, thereby confirming a species boundary through spatial niche partitioning into inshore (A. americanus) and offshore (A. dubius) sand lance species on the NWA. |
author2 |
Miller-Saunders, Kristi Bureau of Ocean Energy Management NSF OCE ERC UConn College of Liberal Arts and Sciences Department of Marine Sciences |
format |
Article in Journal/Newspaper |
author |
Jones, Lucas F Lou, R Nicolas Murray, Christopher S Robert, Dominique Bourne, Christina M Bouchard, Caroline Kučka, Marek Chan, Y Frank Carlon, David B Wiley, David N Therkildsen, Nina O Baumann, Hannes |
spellingShingle |
Jones, Lucas F Lou, R Nicolas Murray, Christopher S Robert, Dominique Bourne, Christina M Bouchard, Caroline Kučka, Marek Chan, Y Frank Carlon, David B Wiley, David N Therkildsen, Nina O Baumann, Hannes Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
author_facet |
Jones, Lucas F Lou, R Nicolas Murray, Christopher S Robert, Dominique Bourne, Christina M Bouchard, Caroline Kučka, Marek Chan, Y Frank Carlon, David B Wiley, David N Therkildsen, Nina O Baumann, Hannes |
author_sort |
Jones, Lucas F |
title |
Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
title_short |
Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
title_full |
Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
title_fullStr |
Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
title_full_unstemmed |
Two distinct population clusters of northern sand lance ( Ammodytes dubius) on the northwest Atlantic shelf revealed by whole genome sequencing |
title_sort |
two distinct population clusters of northern sand lance ( ammodytes dubius) on the northwest atlantic shelf revealed by whole genome sequencing |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/icesjms/fsac217 https://academic.oup.com/icesjms/article-pdf/80/1/122/48860200/fsac217.pdf |
genre |
Greenland Northwest Atlantic |
genre_facet |
Greenland Northwest Atlantic |
op_source |
ICES Journal of Marine Science volume 80, issue 1, page 122-132 ISSN 1054-3139 1095-9289 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/icesjms/fsac217 |
container_title |
ICES Journal of Marine Science |
_version_ |
1810447500218204160 |