Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes
Abstract Plants must adapt with increasing speed to global warming to maintain their fitness. One rapid adaptation mechanism is epigenetic memory, which may provide organisms sufficient time to adapt to climate change. We studied how the perennial Fragaria vesca adapted to warmer temperatures (28°C...
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Oxford University Press (OUP)
2023
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Online Access: | http://dx.doi.org/10.1093/hr/uhad156 https://academic.oup.com/hr/advance-article-pdf/doi/10.1093/hr/uhad156/51013671/uhad156.pdf https://academic.oup.com/hr/article-pdf/10/9/uhad156/51541162/uhad156.pdf |
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croxfordunivpr:10.1093/hr/uhad156 2023-10-09T21:52:49+02:00 Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes Zhang(张宇鹏), YuPeng Fan, Guangxun Toivainen, Tuomas Tengs, Torstein Yakovlev, Igor Krokene, Paal Hytönen, Timo Fossdal, Carl Gunnar Grini, Paul E. 2023 http://dx.doi.org/10.1093/hr/uhad156 https://academic.oup.com/hr/advance-article-pdf/doi/10.1093/hr/uhad156/51013671/uhad156.pdf https://academic.oup.com/hr/article-pdf/10/9/uhad156/51541162/uhad156.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Horticulture Research volume 10, issue 9 ISSN 2052-7276 Horticulture Plant Science Genetics Biochemistry Biotechnology journal-article 2023 croxfordunivpr https://doi.org/10.1093/hr/uhad156 2023-09-22T11:14:44Z Abstract Plants must adapt with increasing speed to global warming to maintain their fitness. One rapid adaptation mechanism is epigenetic memory, which may provide organisms sufficient time to adapt to climate change. We studied how the perennial Fragaria vesca adapted to warmer temperatures (28°C vs. 18°C) over three asexual generations. Differences in flowering time, stolon number, and petiole length were induced by warmer temperature in one or more ecotypes after three asexual generations and persisted in a common garden environment. Induced methylome changes differed between the four ecotypes from Norway, Iceland, Italy, and Spain, but shared methylome responses were also identified. Most differentially methylated regions (DMRs) occurred in the CHG context, and most CHG and CHH DMRs were hypermethylated at the warmer temperature. In eight CHG DMR peaks, a highly similar methylation pattern could be observed between ecotypes. On average, 13% of the differentially methylated genes between ecotypes also showed a temperature-induced change in gene expression. We observed ecotype-specific methylation and expression patterns for genes related to gibberellin metabolism, flowering time, and epigenetic mechanisms. Furthermore, we observed a negative correlation with gene expression when repetitive elements were found near (±2 kb) or inside genes. In conclusion, lasting phenotypic changes indicative of an epigenetic memory were induced by warmer temperature and were accompanied by changes in DNA methylation patterns. Both shared methylation patterns and transcriptome differences between F. vesca accessions were observed, indicating that DNA methylation may be involved in both general and ecotype-specific phenotypic variation. Article in Journal/Newspaper Iceland Oxford University Press (via Crossref) Norway Horticulture Research |
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Open Polar |
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Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Horticulture Plant Science Genetics Biochemistry Biotechnology |
spellingShingle |
Horticulture Plant Science Genetics Biochemistry Biotechnology Zhang(张宇鹏), YuPeng Fan, Guangxun Toivainen, Tuomas Tengs, Torstein Yakovlev, Igor Krokene, Paal Hytönen, Timo Fossdal, Carl Gunnar Grini, Paul E. Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
topic_facet |
Horticulture Plant Science Genetics Biochemistry Biotechnology |
description |
Abstract Plants must adapt with increasing speed to global warming to maintain their fitness. One rapid adaptation mechanism is epigenetic memory, which may provide organisms sufficient time to adapt to climate change. We studied how the perennial Fragaria vesca adapted to warmer temperatures (28°C vs. 18°C) over three asexual generations. Differences in flowering time, stolon number, and petiole length were induced by warmer temperature in one or more ecotypes after three asexual generations and persisted in a common garden environment. Induced methylome changes differed between the four ecotypes from Norway, Iceland, Italy, and Spain, but shared methylome responses were also identified. Most differentially methylated regions (DMRs) occurred in the CHG context, and most CHG and CHH DMRs were hypermethylated at the warmer temperature. In eight CHG DMR peaks, a highly similar methylation pattern could be observed between ecotypes. On average, 13% of the differentially methylated genes between ecotypes also showed a temperature-induced change in gene expression. We observed ecotype-specific methylation and expression patterns for genes related to gibberellin metabolism, flowering time, and epigenetic mechanisms. Furthermore, we observed a negative correlation with gene expression when repetitive elements were found near (±2 kb) or inside genes. In conclusion, lasting phenotypic changes indicative of an epigenetic memory were induced by warmer temperature and were accompanied by changes in DNA methylation patterns. Both shared methylation patterns and transcriptome differences between F. vesca accessions were observed, indicating that DNA methylation may be involved in both general and ecotype-specific phenotypic variation. |
format |
Article in Journal/Newspaper |
author |
Zhang(张宇鹏), YuPeng Fan, Guangxun Toivainen, Tuomas Tengs, Torstein Yakovlev, Igor Krokene, Paal Hytönen, Timo Fossdal, Carl Gunnar Grini, Paul E. |
author_facet |
Zhang(张宇鹏), YuPeng Fan, Guangxun Toivainen, Tuomas Tengs, Torstein Yakovlev, Igor Krokene, Paal Hytönen, Timo Fossdal, Carl Gunnar Grini, Paul E. |
author_sort |
Zhang(张宇鹏), YuPeng |
title |
Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
title_short |
Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
title_full |
Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
title_fullStr |
Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
title_full_unstemmed |
Warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in Fragaria vesca ecotypes |
title_sort |
warmer temperature during asexual reproduction induce methylome, transcriptomic, and lasting phenotypic changes in fragaria vesca ecotypes |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/hr/uhad156 https://academic.oup.com/hr/advance-article-pdf/doi/10.1093/hr/uhad156/51013671/uhad156.pdf https://academic.oup.com/hr/article-pdf/10/9/uhad156/51541162/uhad156.pdf |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Horticulture Research volume 10, issue 9 ISSN 2052-7276 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/hr/uhad156 |
container_title |
Horticulture Research |
_version_ |
1779315999326076928 |