Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci
Abstract Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple in...
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Online Access: | http://dx.doi.org/10.1093/genetics/151.1.285 https://academic.oup.com/genetics/article-pdf/151/1/285/42012209/genetics0285.pdf |
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croxfordunivpr:10.1093/genetics/151.1.285 2024-01-14T10:05:38+01:00 Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci Palsbøll, Per J Bérubé, Martine Jørgensen, Hanne 1999 http://dx.doi.org/10.1093/genetics/151.1.285 https://academic.oup.com/genetics/article-pdf/151/1/285/42012209/genetics0285.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Genetics volume 151, issue 1, page 285-296 ISSN 1943-2631 Genetics journal-article 1999 croxfordunivpr https://doi.org/10.1093/genetics/151.1.285 2023-12-15T09:27:10Z Abstract Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple integer multiples of the basic repeat length. The estimate of the average number of alleles and heterozygosity was higher at loci with imperfect allele arrays relative to those with perfect allele arrays. Nucleotide sequences of 23 different alleles at one tetranucleotide repeat microsatellite locus in fin whales, Balaenoptera physalus, and humpback whales, Megaptera novaeangliae, revealed sequence changes including perfect repeats only, multiple repeats, and partial repeats. The relative rate of the latter two categories of mutation was estimated at 0.024 of the mutation rate involving perfect repeats only. It is hypothesized that single-strand slippage of partial repeats may provide a mechanism for counteracting the continuous expansion of microsatellite loci, which is the logical consequence of recent reports demonstrating directional mutations. Partial-repeat mutations introduce imperfections in the repeat array, which subsequently could reduce the rate of single-strand slippage. Limited computer simulations confirmed this predicted effect of partial-repeat mutations. Article in Journal/Newspaper Balaenoptera physalus baleen whales Megaptera novaeangliae Oxford University Press (via Crossref) Genetics 151 1 285 296 |
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Oxford University Press (via Crossref) |
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English |
topic |
Genetics |
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Genetics Palsbøll, Per J Bérubé, Martine Jørgensen, Hanne Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
topic_facet |
Genetics |
description |
Abstract Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple integer multiples of the basic repeat length. The estimate of the average number of alleles and heterozygosity was higher at loci with imperfect allele arrays relative to those with perfect allele arrays. Nucleotide sequences of 23 different alleles at one tetranucleotide repeat microsatellite locus in fin whales, Balaenoptera physalus, and humpback whales, Megaptera novaeangliae, revealed sequence changes including perfect repeats only, multiple repeats, and partial repeats. The relative rate of the latter two categories of mutation was estimated at 0.024 of the mutation rate involving perfect repeats only. It is hypothesized that single-strand slippage of partial repeats may provide a mechanism for counteracting the continuous expansion of microsatellite loci, which is the logical consequence of recent reports demonstrating directional mutations. Partial-repeat mutations introduce imperfections in the repeat array, which subsequently could reduce the rate of single-strand slippage. Limited computer simulations confirmed this predicted effect of partial-repeat mutations. |
format |
Article in Journal/Newspaper |
author |
Palsbøll, Per J Bérubé, Martine Jørgensen, Hanne |
author_facet |
Palsbøll, Per J Bérubé, Martine Jørgensen, Hanne |
author_sort |
Palsbøll, Per J |
title |
Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
title_short |
Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
title_full |
Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
title_fullStr |
Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
title_full_unstemmed |
Multiple Levels of Single-Strand Slippage at Cetacean Tri- and Tetranucleotide Repeat Microsatellite Loci |
title_sort |
multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
publisher |
Oxford University Press (OUP) |
publishDate |
1999 |
url |
http://dx.doi.org/10.1093/genetics/151.1.285 https://academic.oup.com/genetics/article-pdf/151/1/285/42012209/genetics0285.pdf |
genre |
Balaenoptera physalus baleen whales Megaptera novaeangliae |
genre_facet |
Balaenoptera physalus baleen whales Megaptera novaeangliae |
op_source |
Genetics volume 151, issue 1, page 285-296 ISSN 1943-2631 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/genetics/151.1.285 |
container_title |
Genetics |
container_volume |
151 |
container_issue |
1 |
container_start_page |
285 |
op_container_end_page |
296 |
_version_ |
1788059982357331968 |