Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus
Abstract Damselfishes (Family: Pomacentridae) are a group of ecologically important, primarily coral reef fishes that include over 400 species. Damselfishes have been used as model organisms to study recruitment (anemonefishes), the effects of ocean acidification (spiny damselfish), population struc...
Published in: | G3 Genes|Genomes|Genetics |
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Oxford University Press (OUP)
2023
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croxfordunivpr:10.1093/g3journal/jkac339 2024-05-19T07:46:36+00:00 Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus Roberts, May B Schultz, Darrin T Gatins, Remy Escalona, Merly Bernardi, Giacomo de Koning, D J Marilyn C. Davis Scholarship American Association of University Women Burnand-Partridge Foundation US National Science Foundation 2023 http://dx.doi.org/10.1093/g3journal/jkac339 https://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkac339/49507643/jkac339.pdf https://academic.oup.com/g3journal/article-pdf/13/4/jkac339/56716489/jkac339.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ G3: Genes, Genomes, Genetics volume 13, issue 4 ISSN 2160-1836 journal-article 2023 croxfordunivpr https://doi.org/10.1093/g3journal/jkac339 2024-04-25T07:57:51Z Abstract Damselfishes (Family: Pomacentridae) are a group of ecologically important, primarily coral reef fishes that include over 400 species. Damselfishes have been used as model organisms to study recruitment (anemonefishes), the effects of ocean acidification (spiny damselfish), population structure, and speciation (Dascyllus). The genus Dascyllus includes a group of small-bodied species, and a complex of relatively larger bodied species, the Dascyllus trimaculatus species complex that is comprised of several species including D. trimaculatus itself. The three-spot damselfish, D. trimaculatus, is a widespread and common coral reef fish species found across the tropical Indo-Pacific. Here, we present the first-genome assembly of this species. This assembly contains 910 Mb, 90% of the bases are in 24 chromosome-scale scaffolds, and the Benchmarking Universal Single-Copy Orthologs score of the assembly is 97.9%. Our findings confirm previous reports of a karyotype of 2n = 47 in D. trimaculatus in which one parent contributes 24 chromosomes and the other 23. We find evidence that this karyotype is the result of a heterozygous Robertsonian fusion. We also find that the D. trimaculatus chromosomes are each homologous with single chromosomes of the closely related clownfish species, Amphiprion percula. This assembly will be a valuable resource in the population genomics and conservation of Damselfishes, and continued studies of the karyotypic diversity in this clade. Article in Journal/Newspaper Ocean acidification Oxford University Press G3 Genes|Genomes|Genetics 13 4 |
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Open Polar |
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Oxford University Press |
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English |
description |
Abstract Damselfishes (Family: Pomacentridae) are a group of ecologically important, primarily coral reef fishes that include over 400 species. Damselfishes have been used as model organisms to study recruitment (anemonefishes), the effects of ocean acidification (spiny damselfish), population structure, and speciation (Dascyllus). The genus Dascyllus includes a group of small-bodied species, and a complex of relatively larger bodied species, the Dascyllus trimaculatus species complex that is comprised of several species including D. trimaculatus itself. The three-spot damselfish, D. trimaculatus, is a widespread and common coral reef fish species found across the tropical Indo-Pacific. Here, we present the first-genome assembly of this species. This assembly contains 910 Mb, 90% of the bases are in 24 chromosome-scale scaffolds, and the Benchmarking Universal Single-Copy Orthologs score of the assembly is 97.9%. Our findings confirm previous reports of a karyotype of 2n = 47 in D. trimaculatus in which one parent contributes 24 chromosomes and the other 23. We find evidence that this karyotype is the result of a heterozygous Robertsonian fusion. We also find that the D. trimaculatus chromosomes are each homologous with single chromosomes of the closely related clownfish species, Amphiprion percula. This assembly will be a valuable resource in the population genomics and conservation of Damselfishes, and continued studies of the karyotypic diversity in this clade. |
author2 |
de Koning, D J Marilyn C. Davis Scholarship American Association of University Women Burnand-Partridge Foundation US National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Roberts, May B Schultz, Darrin T Gatins, Remy Escalona, Merly Bernardi, Giacomo |
spellingShingle |
Roberts, May B Schultz, Darrin T Gatins, Remy Escalona, Merly Bernardi, Giacomo Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
author_facet |
Roberts, May B Schultz, Darrin T Gatins, Remy Escalona, Merly Bernardi, Giacomo |
author_sort |
Roberts, May B |
title |
Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
title_short |
Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
title_full |
Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
title_fullStr |
Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
title_full_unstemmed |
Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus |
title_sort |
chromosome-level genome of the three-spot damselfish, dascyllus trimaculatus |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/g3journal/jkac339 https://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkac339/49507643/jkac339.pdf https://academic.oup.com/g3journal/article-pdf/13/4/jkac339/56716489/jkac339.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
G3: Genes, Genomes, Genetics volume 13, issue 4 ISSN 2160-1836 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/g3journal/jkac339 |
container_title |
G3 Genes|Genomes|Genetics |
container_volume |
13 |
container_issue |
4 |
_version_ |
1799486817730297856 |