Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar)
Abstract The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the bre...
Published in: | G3 Genes|Genomes|Genetics |
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Oxford University Press (OUP)
2021
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Online Access: | http://dx.doi.org/10.1093/g3journal/jkab072 http://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkab072/36941135/jkab072.pdf http://academic.oup.com/g3journal/article-pdf/11/4/jkab072/37083043/jkab072.pdf |
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croxfordunivpr:10.1093/g3journal/jkab072 2024-09-15T17:56:32+00:00 Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) Iversen, Marianne Mulugeta, Teshome West, Alexander C Jørgensen, Even H Martin, Samuel A M Sandve, Simen Rød Hazlerigg, David Houston, R D Norway for funding 2021 http://dx.doi.org/10.1093/g3journal/jkab072 http://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkab072/36941135/jkab072.pdf http://academic.oup.com/g3journal/article-pdf/11/4/jkab072/37083043/jkab072.pdf en eng Oxford University Press (OUP) http://creativecommons.org/licenses/by/4.0/ G3 Genes|Genomes|Genetics volume 11, issue 4 ISSN 2160-1836 journal-article 2021 croxfordunivpr https://doi.org/10.1093/g3journal/jkab072 2024-08-27T04:15:46Z Abstract The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the breadth of transcriptional change which takes place in the gill in the FW phase of smoltification. This highlighted the importance of extended exposure to short, winter-like photoperiods (SP) followed by a subsequent increase in photoperiod for completion of transcriptional reprogramming in FW and efficient growth following transfer to SW. Here, we extend this analysis to examine the consequences of this photoperiodic history-dependent reprogramming for subsequent gill responses upon exposure to SW. We use RNAseq to analyze gill samples taken from fish raised on the photoperiod regimes we used previously and then challenged by SW exposure for 24 hours. While fish held on constant light (LL) throughout were able to hypo-osmoregulate during a 24 hours SW challenge, the associated gill transcriptional response was highly distinctive from that in fish which had experienced a 7-week period of exposure to SP followed by a return to LL (SPLL) and had consequently acquired the characteristics of fully developed smolts. Fish transferred from LL to SP, and then held on SP for the remainder of the study was unable to hypo-osmoregulate, and the associated gill transcriptional response to SW exposure featured many transcripts apparently regulated by the glucocorticoid stress axis and by the osmo-sensing transcription factor NFAT5. The importance of these pathways for the gill transcriptional response to SW exposure appears to diminish as a consequence of photoperiod mediated induction of the smolt phenotype, presumably reflecting preparatory developmental changes taking place during this process. Article in Journal/Newspaper Atlantic salmon Salmo salar Oxford University Press G3 Genes|Genomes|Genetics 11 4 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract The developmental transition of juvenile salmon from a freshwater resident morph (parr) to a seawater (SW) migratory morph (smolt), known as smoltification, entails a reorganization of gill function to cope with the altered water environment. Recently, we used RNAseq to characterize the breadth of transcriptional change which takes place in the gill in the FW phase of smoltification. This highlighted the importance of extended exposure to short, winter-like photoperiods (SP) followed by a subsequent increase in photoperiod for completion of transcriptional reprogramming in FW and efficient growth following transfer to SW. Here, we extend this analysis to examine the consequences of this photoperiodic history-dependent reprogramming for subsequent gill responses upon exposure to SW. We use RNAseq to analyze gill samples taken from fish raised on the photoperiod regimes we used previously and then challenged by SW exposure for 24 hours. While fish held on constant light (LL) throughout were able to hypo-osmoregulate during a 24 hours SW challenge, the associated gill transcriptional response was highly distinctive from that in fish which had experienced a 7-week period of exposure to SP followed by a return to LL (SPLL) and had consequently acquired the characteristics of fully developed smolts. Fish transferred from LL to SP, and then held on SP for the remainder of the study was unable to hypo-osmoregulate, and the associated gill transcriptional response to SW exposure featured many transcripts apparently regulated by the glucocorticoid stress axis and by the osmo-sensing transcription factor NFAT5. The importance of these pathways for the gill transcriptional response to SW exposure appears to diminish as a consequence of photoperiod mediated induction of the smolt phenotype, presumably reflecting preparatory developmental changes taking place during this process. |
author2 |
Houston, R D Norway for funding |
format |
Article in Journal/Newspaper |
author |
Iversen, Marianne Mulugeta, Teshome West, Alexander C Jørgensen, Even H Martin, Samuel A M Sandve, Simen Rød Hazlerigg, David |
spellingShingle |
Iversen, Marianne Mulugeta, Teshome West, Alexander C Jørgensen, Even H Martin, Samuel A M Sandve, Simen Rød Hazlerigg, David Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
author_facet |
Iversen, Marianne Mulugeta, Teshome West, Alexander C Jørgensen, Even H Martin, Samuel A M Sandve, Simen Rød Hazlerigg, David |
author_sort |
Iversen, Marianne |
title |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
title_short |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
title_full |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
title_fullStr |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
title_full_unstemmed |
Photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in Atlantic salmon ( Salmo salar) |
title_sort |
photoperiod-dependent developmental reprogramming of the transcriptional response to seawater entry in atlantic salmon ( salmo salar) |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/g3journal/jkab072 http://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkab072/36941135/jkab072.pdf http://academic.oup.com/g3journal/article-pdf/11/4/jkab072/37083043/jkab072.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
G3 Genes|Genomes|Genetics volume 11, issue 4 ISSN 2160-1836 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/g3journal/jkab072 |
container_title |
G3 Genes|Genomes|Genetics |
container_volume |
11 |
container_issue |
4 |
_version_ |
1810432738250981376 |