Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols
Abstract In the context of global warming, the melting of Arctic permafrost raises the threat of a reemergence of microorganisms some of which were shown to remain viable in ancient frozen soils for up to half a million years. In order to evaluate this risk, it is of interest to acquire a better kno...
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/femsml/uqac003 https://academic.oup.com/microlife/advance-article-pdf/doi/10.1093/femsml/uqac003/43296909/uqac003.pdf https://academic.oup.com/microlife/article-pdf/doi/10.1093/femsml/uqac003/49846866/uqac003.pdf |
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croxfordunivpr:10.1093/femsml/uqac003 2024-04-07T07:49:53+00:00 Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols Rigou, Sofia Christo-Foroux, Eugène Santini, Sébastien Goncharov, Artemiy Strauss, Jens Grosse, Guido Fedorov, Alexander N Labadie, Karine Abergel, Chantal Claverie, Jean-Michel Agence Nationale de la Recherche CNRS European Research Council 2022 http://dx.doi.org/10.1093/femsml/uqac003 https://academic.oup.com/microlife/advance-article-pdf/doi/10.1093/femsml/uqac003/43296909/uqac003.pdf https://academic.oup.com/microlife/article-pdf/doi/10.1093/femsml/uqac003/49846866/uqac003.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by-nc/4.0/ microLife volume 3 ISSN 2633-6693 General Medicine journal-article 2022 croxfordunivpr https://doi.org/10.1093/femsml/uqac003 2024-03-08T03:07:25Z Abstract In the context of global warming, the melting of Arctic permafrost raises the threat of a reemergence of microorganisms some of which were shown to remain viable in ancient frozen soils for up to half a million years. In order to evaluate this risk, it is of interest to acquire a better knowledge of the composition of the microbial communities found in this understudied environment. Here, we present a metagenomic analysis of 12 soil samples from Russian Arctic and subarctic pristine areas: Chukotka, Yakutia and Kamchatka, including nine permafrost samples collected at various depths. These large datasets (9.2 × 1011 total bp) were assembled (525 313 contigs > 5 kb), their encoded protein contents predicted, and then used to perform taxonomical assignments of bacterial, archaeal and eukaryotic organisms, as well as DNA viruses. The various samples exhibited variable DNA contents and highly diverse taxonomic profiles showing no obvious relationship with their locations, depths or deposit ages. Bacteria represented the largely dominant DNA fraction (95%) in all samples, followed by archaea (3.2%), surprisingly little eukaryotes (0.5%), and viruses (0.4%). Although no common taxonomic pattern was identified, the samples shared unexpected high frequencies of β-lactamase genes, almost 0.9 copy/bacterial genome. In addition to known environmental threats, the particularly intense warming of the Arctic might thus enhance the spread of bacterial antibiotic resistances, today's major challenge in public health. β-Lactamases were also observed at high frequency in other types of soils, suggesting their general role in the regulation of bacterial populations. Article in Journal/Newspaper Arctic Chukotka Global warming Kamchatka permafrost Subarctic Yakutia Oxford University Press Arctic microLife |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
General Medicine |
spellingShingle |
General Medicine Rigou, Sofia Christo-Foroux, Eugène Santini, Sébastien Goncharov, Artemiy Strauss, Jens Grosse, Guido Fedorov, Alexander N Labadie, Karine Abergel, Chantal Claverie, Jean-Michel Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
topic_facet |
General Medicine |
description |
Abstract In the context of global warming, the melting of Arctic permafrost raises the threat of a reemergence of microorganisms some of which were shown to remain viable in ancient frozen soils for up to half a million years. In order to evaluate this risk, it is of interest to acquire a better knowledge of the composition of the microbial communities found in this understudied environment. Here, we present a metagenomic analysis of 12 soil samples from Russian Arctic and subarctic pristine areas: Chukotka, Yakutia and Kamchatka, including nine permafrost samples collected at various depths. These large datasets (9.2 × 1011 total bp) were assembled (525 313 contigs > 5 kb), their encoded protein contents predicted, and then used to perform taxonomical assignments of bacterial, archaeal and eukaryotic organisms, as well as DNA viruses. The various samples exhibited variable DNA contents and highly diverse taxonomic profiles showing no obvious relationship with their locations, depths or deposit ages. Bacteria represented the largely dominant DNA fraction (95%) in all samples, followed by archaea (3.2%), surprisingly little eukaryotes (0.5%), and viruses (0.4%). Although no common taxonomic pattern was identified, the samples shared unexpected high frequencies of β-lactamase genes, almost 0.9 copy/bacterial genome. In addition to known environmental threats, the particularly intense warming of the Arctic might thus enhance the spread of bacterial antibiotic resistances, today's major challenge in public health. β-Lactamases were also observed at high frequency in other types of soils, suggesting their general role in the regulation of bacterial populations. |
author2 |
Agence Nationale de la Recherche CNRS European Research Council |
format |
Article in Journal/Newspaper |
author |
Rigou, Sofia Christo-Foroux, Eugène Santini, Sébastien Goncharov, Artemiy Strauss, Jens Grosse, Guido Fedorov, Alexander N Labadie, Karine Abergel, Chantal Claverie, Jean-Michel |
author_facet |
Rigou, Sofia Christo-Foroux, Eugène Santini, Sébastien Goncharov, Artemiy Strauss, Jens Grosse, Guido Fedorov, Alexander N Labadie, Karine Abergel, Chantal Claverie, Jean-Michel |
author_sort |
Rigou, Sofia |
title |
Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
title_short |
Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
title_full |
Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
title_fullStr |
Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
title_full_unstemmed |
Metagenomic survey of the microbiome of ancient Siberian permafrost and modern Kamchatkan cryosols |
title_sort |
metagenomic survey of the microbiome of ancient siberian permafrost and modern kamchatkan cryosols |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/femsml/uqac003 https://academic.oup.com/microlife/advance-article-pdf/doi/10.1093/femsml/uqac003/43296909/uqac003.pdf https://academic.oup.com/microlife/article-pdf/doi/10.1093/femsml/uqac003/49846866/uqac003.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Chukotka Global warming Kamchatka permafrost Subarctic Yakutia |
genre_facet |
Arctic Chukotka Global warming Kamchatka permafrost Subarctic Yakutia |
op_source |
microLife volume 3 ISSN 2633-6693 |
op_rights |
https://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1093/femsml/uqac003 |
container_title |
microLife |
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1795664408119083008 |