Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming
ABSTRACT Thermokarst lakes are large potential greenhouse gas (GHG) sources in a changing Arctic. In a warming world, an increase in both organic matter availability and temperature is expected to boost methanogenesis and potentially alter the microbial community that controls GHG fluxes. These comm...
Published in: | FEMS Microbes |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1093/femsmc/xtaa008 http://academic.oup.com/femsmicrobes/advance-article-pdf/doi/10.1093/femsmc/xtaa008/34577628/xtaa008.pdf https://academic.oup.com/femsmicrobes/article-pdf/1/1/xtaa008/49847460/xtaa008.pdf |
id |
croxfordunivpr:10.1093/femsmc/xtaa008 |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1093/femsmc/xtaa008 2024-01-28T10:03:22+01:00 Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming in 't Zandt, Michiel H Frank, Jeroen Yilmaz, Polen Cremers, Geert Jetten, Mike S M Welte, Cornelia U Netherlands Organisation for Scientific Research Netherlands Earth System Science Centre European Research Council 2020 http://dx.doi.org/10.1093/femsmc/xtaa008 http://academic.oup.com/femsmicrobes/advance-article-pdf/doi/10.1093/femsmc/xtaa008/34577628/xtaa008.pdf https://academic.oup.com/femsmicrobes/article-pdf/1/1/xtaa008/49847460/xtaa008.pdf en eng Oxford University Press (OUP) http://creativecommons.org/licenses/by/4.0/ FEMS Microbes volume 1, issue 1 ISSN 2633-6685 General Earth and Planetary Sciences General Engineering General Environmental Science journal-article 2020 croxfordunivpr https://doi.org/10.1093/femsmc/xtaa008 2023-12-29T09:30:30Z ABSTRACT Thermokarst lakes are large potential greenhouse gas (GHG) sources in a changing Arctic. In a warming world, an increase in both organic matter availability and temperature is expected to boost methanogenesis and potentially alter the microbial community that controls GHG fluxes. These community shifts are, however, challenging to detect by resolution-limited 16S rRNA gene-based approaches. Here, we applied full metagenome sequencing on long-term thermokarst lake sediment enrichments on acetate and trimethylamine at 4°C and 10°C to unravel species-specific responses to the most likely Arctic climate change scenario. Substrate amendment was used to mimic the increased organic carbon availability upon permafrost thaw. By performing de novo assembly, we reconstructed five high-quality and five medium-quality metagenome-assembled genomes (MAGs) that represented 59% of the aligned metagenome reads. Seven bacterial MAGs belonged to anaerobic fermentative bacteria. Within the Archaea, the enrichment of methanogenic Methanosaetaceae/Methanotrichaceae under acetate amendment and Methanosarcinaceae under trimethylamine (TMA) amendment was not unexpected. Surprisingly, we observed temperature-specific methanogenic (sub)species responses with TMA amendment. These highlighted distinct and potentially functional climate-induced shifts could not be revealed with 16S rRNA gene-based analyses. Unraveling these temperature- and nutrient-controlled species-level responses is essential to better comprehend the mechanisms that underlie GHG production from Arctic lakes in a warming world. Article in Journal/Newspaper Arctic Climate change permafrost Thermokarst Oxford University Press (via Crossref) Arctic FEMS Microbes 1 1 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
General Earth and Planetary Sciences General Engineering General Environmental Science |
spellingShingle |
General Earth and Planetary Sciences General Engineering General Environmental Science in 't Zandt, Michiel H Frank, Jeroen Yilmaz, Polen Cremers, Geert Jetten, Mike S M Welte, Cornelia U Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
topic_facet |
General Earth and Planetary Sciences General Engineering General Environmental Science |
description |
ABSTRACT Thermokarst lakes are large potential greenhouse gas (GHG) sources in a changing Arctic. In a warming world, an increase in both organic matter availability and temperature is expected to boost methanogenesis and potentially alter the microbial community that controls GHG fluxes. These community shifts are, however, challenging to detect by resolution-limited 16S rRNA gene-based approaches. Here, we applied full metagenome sequencing on long-term thermokarst lake sediment enrichments on acetate and trimethylamine at 4°C and 10°C to unravel species-specific responses to the most likely Arctic climate change scenario. Substrate amendment was used to mimic the increased organic carbon availability upon permafrost thaw. By performing de novo assembly, we reconstructed five high-quality and five medium-quality metagenome-assembled genomes (MAGs) that represented 59% of the aligned metagenome reads. Seven bacterial MAGs belonged to anaerobic fermentative bacteria. Within the Archaea, the enrichment of methanogenic Methanosaetaceae/Methanotrichaceae under acetate amendment and Methanosarcinaceae under trimethylamine (TMA) amendment was not unexpected. Surprisingly, we observed temperature-specific methanogenic (sub)species responses with TMA amendment. These highlighted distinct and potentially functional climate-induced shifts could not be revealed with 16S rRNA gene-based analyses. Unraveling these temperature- and nutrient-controlled species-level responses is essential to better comprehend the mechanisms that underlie GHG production from Arctic lakes in a warming world. |
author2 |
Netherlands Organisation for Scientific Research Netherlands Earth System Science Centre European Research Council |
format |
Article in Journal/Newspaper |
author |
in 't Zandt, Michiel H Frank, Jeroen Yilmaz, Polen Cremers, Geert Jetten, Mike S M Welte, Cornelia U |
author_facet |
in 't Zandt, Michiel H Frank, Jeroen Yilmaz, Polen Cremers, Geert Jetten, Mike S M Welte, Cornelia U |
author_sort |
in 't Zandt, Michiel H |
title |
Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
title_short |
Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
title_full |
Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
title_fullStr |
Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
title_full_unstemmed |
Long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
title_sort |
long-term enriched methanogenic communities from thermokarst lake sediments show species-specific responses to warming |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1093/femsmc/xtaa008 http://academic.oup.com/femsmicrobes/advance-article-pdf/doi/10.1093/femsmc/xtaa008/34577628/xtaa008.pdf https://academic.oup.com/femsmicrobes/article-pdf/1/1/xtaa008/49847460/xtaa008.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change permafrost Thermokarst |
genre_facet |
Arctic Climate change permafrost Thermokarst |
op_source |
FEMS Microbes volume 1, issue 1 ISSN 2633-6685 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsmc/xtaa008 |
container_title |
FEMS Microbes |
container_volume |
1 |
container_issue |
1 |
_version_ |
1789328766760124416 |