Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean
Abstract Yeasts are prevalent in the open ocean, yet we have limited understanding of their ecophysiological adaptations, including their response to nitrogen availability, which can have a major role in determining the ecological potential of other planktonic microbes. In this study, we characteriz...
Published in: | FEMS Microbiology Ecology |
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Oxford University Press (OUP)
2024
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Online Access: | http://dx.doi.org/10.1093/femsec/fiae053 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiae053/57211908/fiae053.pdf https://academic.oup.com/femsec/article-pdf/100/5/fiae053/57367922/fiae053.pdf |
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croxfordunivpr:10.1093/femsec/fiae053 2024-10-13T14:09:27+00:00 Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean Diver, Poppy Ward, Ben A Cunliffe, Michael Natural Environment Research Council European Research Council 2024 http://dx.doi.org/10.1093/femsec/fiae053 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiae053/57211908/fiae053.pdf https://academic.oup.com/femsec/article-pdf/100/5/fiae053/57367922/fiae053.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology volume 100, issue 5 ISSN 1574-6941 journal-article 2024 croxfordunivpr https://doi.org/10.1093/femsec/fiae053 2024-09-24T04:07:24Z Abstract Yeasts are prevalent in the open ocean, yet we have limited understanding of their ecophysiological adaptations, including their response to nitrogen availability, which can have a major role in determining the ecological potential of other planktonic microbes. In this study, we characterized the nitrogen uptake capabilities and growth responses of marine-occurring yeasts. Yeast isolates from the North Atlantic Ocean were screened for growth on diverse nitrogen substrates, and across a concentration gradient of three environmentally relevant nitrogen substrates: nitrate, ammonium, and urea. Three strains grew with enriched nitrate while two did not, demonstrating that nitrate utilization is present but not universal in marine yeasts, consistent with existing knowledge of nonmarine yeast strains. Naganishia diffluens MBA_F0213 modified the key functional trait of cell size in response to nitrogen concentration, suggesting yeast cell morphology changes along chemical gradients in the marine environment. Meta-analysis of the reference DNA barcode in public databases revealed that the genus Naganishia has a global ocean distribution, strengthening the environmental applicability of the culture-based observations. This study provides novel quantitative understanding of the ecophysiological and morphological responses of marine-derived yeasts to variable nitrogen availability in vitro, providing insight into the functional ecology of yeasts within pelagic open ocean environments. Article in Journal/Newspaper North Atlantic Oxford University Press FEMS Microbiology Ecology |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Yeasts are prevalent in the open ocean, yet we have limited understanding of their ecophysiological adaptations, including their response to nitrogen availability, which can have a major role in determining the ecological potential of other planktonic microbes. In this study, we characterized the nitrogen uptake capabilities and growth responses of marine-occurring yeasts. Yeast isolates from the North Atlantic Ocean were screened for growth on diverse nitrogen substrates, and across a concentration gradient of three environmentally relevant nitrogen substrates: nitrate, ammonium, and urea. Three strains grew with enriched nitrate while two did not, demonstrating that nitrate utilization is present but not universal in marine yeasts, consistent with existing knowledge of nonmarine yeast strains. Naganishia diffluens MBA_F0213 modified the key functional trait of cell size in response to nitrogen concentration, suggesting yeast cell morphology changes along chemical gradients in the marine environment. Meta-analysis of the reference DNA barcode in public databases revealed that the genus Naganishia has a global ocean distribution, strengthening the environmental applicability of the culture-based observations. This study provides novel quantitative understanding of the ecophysiological and morphological responses of marine-derived yeasts to variable nitrogen availability in vitro, providing insight into the functional ecology of yeasts within pelagic open ocean environments. |
author2 |
Natural Environment Research Council European Research Council |
format |
Article in Journal/Newspaper |
author |
Diver, Poppy Ward, Ben A Cunliffe, Michael |
spellingShingle |
Diver, Poppy Ward, Ben A Cunliffe, Michael Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
author_facet |
Diver, Poppy Ward, Ben A Cunliffe, Michael |
author_sort |
Diver, Poppy |
title |
Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
title_short |
Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
title_full |
Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
title_fullStr |
Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
title_full_unstemmed |
Physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
title_sort |
physiological and morphological plasticity in response to nitrogen availability of a yeast widely distributed in the open ocean |
publisher |
Oxford University Press (OUP) |
publishDate |
2024 |
url |
http://dx.doi.org/10.1093/femsec/fiae053 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiae053/57211908/fiae053.pdf https://academic.oup.com/femsec/article-pdf/100/5/fiae053/57367922/fiae053.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
FEMS Microbiology Ecology volume 100, issue 5 ISSN 1574-6941 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiae053 |
container_title |
FEMS Microbiology Ecology |
_version_ |
1812816432510009344 |