Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
Abstract The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent...
Published in: | FEMS Microbiology Ecology |
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Language: | English |
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Oxford University Press (OUP)
2023
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Online Access: | http://dx.doi.org/10.1093/femsec/fiad119 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad119/51846653/fiad119.pdf https://academic.oup.com/femsec/article-pdf/99/11/fiad119/53401703/fiad119.pdf |
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croxfordunivpr:10.1093/femsec/fiad119 2024-05-12T08:04:28+00:00 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches Jaarsma, Ate H Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane S Stougaard, Peter Benning, Liane G Tranter, Martyn Anesio, Alexandre M European Research Council 2023 http://dx.doi.org/10.1093/femsec/fiad119 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad119/51846653/fiad119.pdf https://academic.oup.com/femsec/article-pdf/99/11/fiad119/53401703/fiad119.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology volume 99, issue 11 ISSN 1574-6941 Applied Microbiology and Biotechnology Ecology Microbiology journal-article 2023 croxfordunivpr https://doi.org/10.1093/femsec/fiad119 2024-04-18T08:15:04Z Abstract The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. Article in Journal/Newspaper Greenland Ice Sheet Oxford University Press Greenland FEMS Microbiology Ecology 99 11 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Applied Microbiology and Biotechnology Ecology Microbiology |
spellingShingle |
Applied Microbiology and Biotechnology Ecology Microbiology Jaarsma, Ate H Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane S Stougaard, Peter Benning, Liane G Tranter, Martyn Anesio, Alexandre M Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
topic_facet |
Applied Microbiology and Biotechnology Ecology Microbiology |
description |
Abstract The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. |
author2 |
European Research Council |
format |
Article in Journal/Newspaper |
author |
Jaarsma, Ate H Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane S Stougaard, Peter Benning, Liane G Tranter, Martyn Anesio, Alexandre M |
author_facet |
Jaarsma, Ate H Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane S Stougaard, Peter Benning, Liane G Tranter, Martyn Anesio, Alexandre M |
author_sort |
Jaarsma, Ate H |
title |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_short |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_fullStr |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full_unstemmed |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_sort |
exploring microbial diversity in greenland ice sheet supraglacial habitats through culturing-dependent and -independent approaches |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/femsec/fiad119 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad119/51846653/fiad119.pdf https://academic.oup.com/femsec/article-pdf/99/11/fiad119/53401703/fiad119.pdf |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
FEMS Microbiology Ecology volume 99, issue 11 ISSN 1574-6941 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiad119 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
11 |
_version_ |
1798846678400237568 |