Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes
Abstract Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morpho...
Published in: | FEMS Microbiology Ecology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2023
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1093/femsec/fiad057 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad057/50449952/fiad057.pdf https://academic.oup.com/femsec/article-pdf/99/6/fiad057/50530440/fiad057.pdf |
id |
croxfordunivpr:10.1093/femsec/fiad057 |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1093/femsec/fiad057 2024-10-13T14:09:45+00:00 Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J Norwegian Research Council European Commission Marie Skłodowska-Curie individual Charles University Research Centre program INTER-EXCELLENCE project Institute of Botany, CAS 2023 http://dx.doi.org/10.1093/femsec/fiad057 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad057/50449952/fiad057.pdf https://academic.oup.com/femsec/article-pdf/99/6/fiad057/50530440/fiad057.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology volume 99, issue 6 ISSN 1574-6941 journal-article 2023 croxfordunivpr https://doi.org/10.1093/femsec/fiad057 2024-09-24T04:07:13Z Abstract Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. Article in Journal/Newspaper Northern Norway Oxford University Press Norway FEMS Microbiology Ecology 99 6 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. |
author2 |
Norwegian Research Council European Commission Marie Skłodowska-Curie individual Charles University Research Centre program INTER-EXCELLENCE project Institute of Botany, CAS |
format |
Article in Journal/Newspaper |
author |
Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J |
spellingShingle |
Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
author_facet |
Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J |
author_sort |
Suzuki, Hirono |
title |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_short |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_fullStr |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full_unstemmed |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_sort |
phylogeny and lipid profiles of snow-algae isolated from norwegian red-snow microbiomes |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/femsec/fiad057 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad057/50449952/fiad057.pdf https://academic.oup.com/femsec/article-pdf/99/6/fiad057/50530440/fiad057.pdf |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
FEMS Microbiology Ecology volume 99, issue 6 ISSN 1574-6941 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiad057 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
6 |
_version_ |
1812816806772998144 |