Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing
Abstract Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacki...
Published in: | FEMS Microbiology Ecology |
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Oxford University Press (OUP)
2023
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croxfordunivpr:10.1093/femsec/fiad015 2024-04-28T08:01:19+00:00 Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub National Science Centre, Poland 2023 http://dx.doi.org/10.1093/femsec/fiad015 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad015/49119879/fiad015.pdf https://academic.oup.com/femsec/article-pdf/99/3/fiad015/50976239/fiad015.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology volume 99, issue 3 ISSN 1574-6941 Applied Microbiology and Biotechnology Ecology Microbiology journal-article 2023 croxfordunivpr https://doi.org/10.1093/femsec/fiad015 2024-04-02T08:04:51Z Abstract Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In this paper, we assessed the phylogenetic structure of the whole and active fractions of bacterial communities housed by Antarctic lichens growing in different environmental conditions by targeted 16S rRNA gene amplicon sequencing. Bacterial communities associated with lichens procured from a nitrogen enriched site were very distinct from the communities isolated from lichens of a nitrogen depleted site. The former were characterized by substantial contributions of Bacteroidetes phylum members and the elusive Armatimonadetes. At the nutrient-poor site the lichen-associated bacteriobiome structure was unique for each lichen species, with chlorolichens being occupied largely by Proteobacteria. Lichen species with a pronounced discrepancy in diversity between the whole and active fractions of their bacterial communities had the widest ecological amplitude, hinting that the nonactive part of the community is a reservoir of latent stress coping mechanisms. This is the first investigation to make use of targeted metatranscriptomics to infer the bacterial biodiversity in Antarctic lichens. Article in Journal/Newspaper Antarc* Antarctic Oxford University Press FEMS Microbiology Ecology |
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English |
topic |
Applied Microbiology and Biotechnology Ecology Microbiology |
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Applied Microbiology and Biotechnology Ecology Microbiology Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
topic_facet |
Applied Microbiology and Biotechnology Ecology Microbiology |
description |
Abstract Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In this paper, we assessed the phylogenetic structure of the whole and active fractions of bacterial communities housed by Antarctic lichens growing in different environmental conditions by targeted 16S rRNA gene amplicon sequencing. Bacterial communities associated with lichens procured from a nitrogen enriched site were very distinct from the communities isolated from lichens of a nitrogen depleted site. The former were characterized by substantial contributions of Bacteroidetes phylum members and the elusive Armatimonadetes. At the nutrient-poor site the lichen-associated bacteriobiome structure was unique for each lichen species, with chlorolichens being occupied largely by Proteobacteria. Lichen species with a pronounced discrepancy in diversity between the whole and active fractions of their bacterial communities had the widest ecological amplitude, hinting that the nonactive part of the community is a reservoir of latent stress coping mechanisms. This is the first investigation to make use of targeted metatranscriptomics to infer the bacterial biodiversity in Antarctic lichens. |
author2 |
National Science Centre, Poland |
format |
Article in Journal/Newspaper |
author |
Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub |
author_facet |
Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub |
author_sort |
Woltyńska, Aleksandra |
title |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_short |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_full |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_fullStr |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_full_unstemmed |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_sort |
bacterial communities of antarctic lichens explored by gdna and cdna 16s rrna gene amplicon sequencing |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/femsec/fiad015 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiad015/49119879/fiad015.pdf https://academic.oup.com/femsec/article-pdf/99/3/fiad015/50976239/fiad015.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
FEMS Microbiology Ecology volume 99, issue 3 ISSN 1574-6941 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiad015 |
container_title |
FEMS Microbiology Ecology |
_version_ |
1797573113408389120 |