Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands
Abstract Hydrocarbons may have a natural or anthropogenic origin and serve as a source of carbon and energy for microorganisms in Antarctic soils. Herein, 16S rRNA gene and shotgun sequencing were employed to characterize taxonomic diversity and genetic potential for hydrocarbon degradation of the m...
Published in: | FEMS Microbiology Ecology |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/femsec/fiac143 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiac143/47267008/fiac143.pdf https://academic.oup.com/femsec/article-pdf/99/1/fiac143/47884380/fiac143.pdf |
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croxfordunivpr:10.1093/femsec/fiac143 2024-05-19T07:31:43+00:00 Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands Silva, Jéssica B Centurion, Victor B Duarte, Alysson W F Galazzi, Rodrigo M Arruda, Marco A Z Sartoratto, Adilson Rosa, Luiz H Oliveira, Valéria M São Paulo Research Foundation National Council for Scientific and Technological Development 2022 http://dx.doi.org/10.1093/femsec/fiac143 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiac143/47267008/fiac143.pdf https://academic.oup.com/femsec/article-pdf/99/1/fiac143/47884380/fiac143.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model FEMS Microbiology Ecology volume 99, issue 1 ISSN 1574-6941 journal-article 2022 croxfordunivpr https://doi.org/10.1093/femsec/fiac143 2024-04-25T07:59:20Z Abstract Hydrocarbons may have a natural or anthropogenic origin and serve as a source of carbon and energy for microorganisms in Antarctic soils. Herein, 16S rRNA gene and shotgun sequencing were employed to characterize taxonomic diversity and genetic potential for hydrocarbon degradation of the microbiome from sediments of sites located in two Antarctic islands subjected to different temperatures, geochemical compositions, and levels of presumed anthropogenic impact, named: Crater Lake/Deception Island (pristine area), Whalers Bay and Fumarole Bay/Deception Island (anthropogenic-impacted area), and Hannah Point/Livingston Island (anthropogenic-impacted area). Hydrocarbon concentrations were measured for further correlation analyses with biological data. The majority of the hydrocarbon-degrading genes were affiliated to the most abundant bacterial groups of the microbiome: Proteobacteria and Actinobacteria. KEGG annotation revealed 125 catabolic genes related to aromatic hydrocarbon (styrene, toluene, ethylbenzene, xylene, naphthalene, and polycyclic hydrocarbons) and aliphatic (alkanes and cycloalkanes) pathways. Only aliphatic hydrocarbons, in low concentrations, were detected in all areas, thus not characterizing the areas under study as anthropogenically impacted or nonimpacted. The high richness and abundance of hydrocarbon-degrading genes suggest that the genetic potential of the microbiome from Antarctic sediments for hydrocarbon degradation is driven by natural hydrocarbon occurrence. Article in Journal/Newspaper Antarc* Antarctic Deception Island Livingston Island Oxford University Press FEMS Microbiology Ecology 99 1 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Hydrocarbons may have a natural or anthropogenic origin and serve as a source of carbon and energy for microorganisms in Antarctic soils. Herein, 16S rRNA gene and shotgun sequencing were employed to characterize taxonomic diversity and genetic potential for hydrocarbon degradation of the microbiome from sediments of sites located in two Antarctic islands subjected to different temperatures, geochemical compositions, and levels of presumed anthropogenic impact, named: Crater Lake/Deception Island (pristine area), Whalers Bay and Fumarole Bay/Deception Island (anthropogenic-impacted area), and Hannah Point/Livingston Island (anthropogenic-impacted area). Hydrocarbon concentrations were measured for further correlation analyses with biological data. The majority of the hydrocarbon-degrading genes were affiliated to the most abundant bacterial groups of the microbiome: Proteobacteria and Actinobacteria. KEGG annotation revealed 125 catabolic genes related to aromatic hydrocarbon (styrene, toluene, ethylbenzene, xylene, naphthalene, and polycyclic hydrocarbons) and aliphatic (alkanes and cycloalkanes) pathways. Only aliphatic hydrocarbons, in low concentrations, were detected in all areas, thus not characterizing the areas under study as anthropogenically impacted or nonimpacted. The high richness and abundance of hydrocarbon-degrading genes suggest that the genetic potential of the microbiome from Antarctic sediments for hydrocarbon degradation is driven by natural hydrocarbon occurrence. |
author2 |
São Paulo Research Foundation National Council for Scientific and Technological Development |
format |
Article in Journal/Newspaper |
author |
Silva, Jéssica B Centurion, Victor B Duarte, Alysson W F Galazzi, Rodrigo M Arruda, Marco A Z Sartoratto, Adilson Rosa, Luiz H Oliveira, Valéria M |
spellingShingle |
Silva, Jéssica B Centurion, Victor B Duarte, Alysson W F Galazzi, Rodrigo M Arruda, Marco A Z Sartoratto, Adilson Rosa, Luiz H Oliveira, Valéria M Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
author_facet |
Silva, Jéssica B Centurion, Victor B Duarte, Alysson W F Galazzi, Rodrigo M Arruda, Marco A Z Sartoratto, Adilson Rosa, Luiz H Oliveira, Valéria M |
author_sort |
Silva, Jéssica B |
title |
Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
title_short |
Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
title_full |
Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
title_fullStr |
Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
title_full_unstemmed |
Unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of Antarctic islands |
title_sort |
unravelling the genetic potential for hydrocarbon degradation in the sediment microbiome of antarctic islands |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/femsec/fiac143 https://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiac143/47267008/fiac143.pdf https://academic.oup.com/femsec/article-pdf/99/1/fiac143/47884380/fiac143.pdf |
genre |
Antarc* Antarctic Deception Island Livingston Island |
genre_facet |
Antarc* Antarctic Deception Island Livingston Island |
op_source |
FEMS Microbiology Ecology volume 99, issue 1 ISSN 1574-6941 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/femsec/fiac143 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
1 |
_version_ |
1799469597283319808 |