Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost
ABSTRACT Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose mill...
Published in: | FEMS Microbiology Ecology |
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Oxford University Press (OUP)
2020
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Online Access: | http://dx.doi.org/10.1093/femsec/fiaa057 http://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiaa057/33097397/fiaa057.pdf http://academic.oup.com/femsec/article-pdf/96/5/fiaa057/33111233/fiaa057.pdf |
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croxfordunivpr:10.1093/femsec/fiaa057 2024-06-23T07:56:02+00:00 Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan Norwegian Research Council Fulbright Foundation in Greece 2020 http://dx.doi.org/10.1093/femsec/fiaa057 http://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiaa057/33097397/fiaa057.pdf http://academic.oup.com/femsec/article-pdf/96/5/fiaa057/33111233/fiaa057.pdf en eng Oxford University Press (OUP) http://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology volume 96, issue 5 ISSN 0168-6496 1574-6941 journal-article 2020 croxfordunivpr https://doi.org/10.1093/femsec/fiaa057 2024-06-11T04:17:18Z ABSTRACT Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. Article in Journal/Newspaper permafrost Svalbard Oxford University Press Norway Svalbard FEMS Microbiology Ecology 96 5 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
ABSTRACT Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. |
author2 |
Norwegian Research Council Fulbright Foundation in Greece |
format |
Article in Journal/Newspaper |
author |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan |
spellingShingle |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
author_facet |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan |
author_sort |
Xue, Yaxin |
title |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_short |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_fullStr |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full_unstemmed |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_sort |
metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in svalbard permafrost |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1093/femsec/fiaa057 http://academic.oup.com/femsec/advance-article-pdf/doi/10.1093/femsec/fiaa057/33097397/fiaa057.pdf http://academic.oup.com/femsec/article-pdf/96/5/fiaa057/33111233/fiaa057.pdf |
geographic |
Norway Svalbard |
geographic_facet |
Norway Svalbard |
genre |
permafrost Svalbard |
genre_facet |
permafrost Svalbard |
op_source |
FEMS Microbiology Ecology volume 96, issue 5 ISSN 0168-6496 1574-6941 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiaa057 |
container_title |
FEMS Microbiology Ecology |
container_volume |
96 |
container_issue |
5 |
_version_ |
1802648872986607616 |