Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model
Abstract OBJECTIVES Postoperative prolonged air leakage is a frequent complication following lung resection. We have shown the high adhesive quality of a newly developed sealant based on a hydrophobically modified Alaska pollock-derived gelatin (ApGltn) sealant in acute in vivo settings. The purpose...
Published in: | European Journal of Cardio-Thoracic Surgery |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/ejcts/ezac497 https://academic.oup.com/ejcts/advance-article-pdf/doi/10.1093/ejcts/ezac497/46579997/ezac497.pdf https://academic.oup.com/ejcts/article-pdf/62/5/ezac497/46632080/ezac497.pdf |
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croxfordunivpr:10.1093/ejcts/ezac497 2023-05-15T13:09:22+02:00 Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model Yanagihara, Takahiro Maki, Naoki Kawamura, Tomoyuki Kobayashi, Naohiro Kikuchi, Shinji Goto, Yukinobu Ichimura, Hideo Watanabe, Shiharu Taguchi, Tetsushi Sato, Yukio 2022 http://dx.doi.org/10.1093/ejcts/ezac497 https://academic.oup.com/ejcts/advance-article-pdf/doi/10.1093/ejcts/ezac497/46579997/ezac497.pdf https://academic.oup.com/ejcts/article-pdf/62/5/ezac497/46632080/ezac497.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model European Journal of Cardio-Thoracic Surgery volume 62, issue 5 ISSN 1010-7940 1873-734X Cardiology and Cardiovascular Medicine Pulmonary and Respiratory Medicine General Medicine Surgery journal-article 2022 croxfordunivpr https://doi.org/10.1093/ejcts/ezac497 2022-12-29T15:39:03Z Abstract OBJECTIVES Postoperative prolonged air leakage is a frequent complication following lung resection. We have shown the high adhesive quality of a newly developed sealant based on a hydrophobically modified Alaska pollock-derived gelatin (ApGltn) sealant in acute in vivo settings. The purpose of this study was to investigate the long-term efficacy and safety of ApGltn sealant using rats as a preclinical model. METHODS An air leakage rat model with a 5-mm pleural defect was created, to which ApGltn sealant or fibrin sealant was applied. In both groups, the rats were evaluated on days 1, 7, 14 and 28. In the ApGltn sealant group, days 56 and 84 were added to evaluate absorption as sealant was still present on day 28. The number of rats in each subgroup was 4 (for a total of 40). Lung specimens and blood samples were obtained for histological and haematological assessment. RESULTS No findings suggesting infection or air leakage were observed. ApGltn sealant was absorbed from day 56 to day 84. Histologically, although neutrophil and lymphocyte infiltrations on the lung side did not differ between groups, those on the sealant side were significantly less in the ApGltn sealant group. Blood sample tests revealed no significant findings suggesting inflammation or organ damage in either group. CONCLUSIONS ApGltn sealant showed long-term sealing efficacy and safety with mild inflammation in a pulmonary air leakage rat model. ApGltn sealant is expected to be a safe and effective sealant for clinical applications. Article in Journal/Newspaper alaska pollock Alaska Oxford University Press (via Crossref) European Journal of Cardio-Thoracic Surgery 62 5 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Cardiology and Cardiovascular Medicine Pulmonary and Respiratory Medicine General Medicine Surgery |
spellingShingle |
Cardiology and Cardiovascular Medicine Pulmonary and Respiratory Medicine General Medicine Surgery Yanagihara, Takahiro Maki, Naoki Kawamura, Tomoyuki Kobayashi, Naohiro Kikuchi, Shinji Goto, Yukinobu Ichimura, Hideo Watanabe, Shiharu Taguchi, Tetsushi Sato, Yukio Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
topic_facet |
Cardiology and Cardiovascular Medicine Pulmonary and Respiratory Medicine General Medicine Surgery |
description |
Abstract OBJECTIVES Postoperative prolonged air leakage is a frequent complication following lung resection. We have shown the high adhesive quality of a newly developed sealant based on a hydrophobically modified Alaska pollock-derived gelatin (ApGltn) sealant in acute in vivo settings. The purpose of this study was to investigate the long-term efficacy and safety of ApGltn sealant using rats as a preclinical model. METHODS An air leakage rat model with a 5-mm pleural defect was created, to which ApGltn sealant or fibrin sealant was applied. In both groups, the rats were evaluated on days 1, 7, 14 and 28. In the ApGltn sealant group, days 56 and 84 were added to evaluate absorption as sealant was still present on day 28. The number of rats in each subgroup was 4 (for a total of 40). Lung specimens and blood samples were obtained for histological and haematological assessment. RESULTS No findings suggesting infection or air leakage were observed. ApGltn sealant was absorbed from day 56 to day 84. Histologically, although neutrophil and lymphocyte infiltrations on the lung side did not differ between groups, those on the sealant side were significantly less in the ApGltn sealant group. Blood sample tests revealed no significant findings suggesting inflammation or organ damage in either group. CONCLUSIONS ApGltn sealant showed long-term sealing efficacy and safety with mild inflammation in a pulmonary air leakage rat model. ApGltn sealant is expected to be a safe and effective sealant for clinical applications. |
format |
Article in Journal/Newspaper |
author |
Yanagihara, Takahiro Maki, Naoki Kawamura, Tomoyuki Kobayashi, Naohiro Kikuchi, Shinji Goto, Yukinobu Ichimura, Hideo Watanabe, Shiharu Taguchi, Tetsushi Sato, Yukio |
author_facet |
Yanagihara, Takahiro Maki, Naoki Kawamura, Tomoyuki Kobayashi, Naohiro Kikuchi, Shinji Goto, Yukinobu Ichimura, Hideo Watanabe, Shiharu Taguchi, Tetsushi Sato, Yukio |
author_sort |
Yanagihara, Takahiro |
title |
Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
title_short |
Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
title_full |
Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
title_fullStr |
Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
title_full_unstemmed |
Alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
title_sort |
alaska pollock gelatin sealant shows long-term efficacy and safety in a pulmonary air leakage rat model |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/ejcts/ezac497 https://academic.oup.com/ejcts/advance-article-pdf/doi/10.1093/ejcts/ezac497/46579997/ezac497.pdf https://academic.oup.com/ejcts/article-pdf/62/5/ezac497/46632080/ezac497.pdf |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
European Journal of Cardio-Thoracic Surgery volume 62, issue 5 ISSN 1010-7940 1873-734X |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/ejcts/ezac497 |
container_title |
European Journal of Cardio-Thoracic Surgery |
container_volume |
62 |
container_issue |
5 |
_version_ |
1766174269542236160 |