Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas
Abstract Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPi...
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Online Access: | http://dx.doi.org/10.1093/czoolo/59.1.109 http://academic.oup.com/cz/article-pdf/59/1/109/32969460/czoolo59-0109.pdf |
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croxfordunivpr:10.1093/czoolo/59.1.109 2023-09-26T15:17:23+02:00 Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan 2013 http://dx.doi.org/10.1093/czoolo/59.1.109 http://academic.oup.com/cz/article-pdf/59/1/109/32969460/czoolo59-0109.pdf en eng Oxford University Press (OUP) Current Zoology volume 59, issue 1, page 109-115 ISSN 2396-9814 1674-5507 Animal Science and Zoology journal-article 2013 croxfordunivpr https://doi.org/10.1093/czoolo/59.1.109 2023-08-25T11:36:22Z Abstract Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Oxford University Press (via Crossref) Pacific Current Zoology 59 1 109 115 |
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Oxford University Press (via Crossref) |
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topic |
Animal Science and Zoology |
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Animal Science and Zoology Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
topic_facet |
Animal Science and Zoology |
description |
Abstract Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species. |
format |
Article in Journal/Newspaper |
author |
Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan |
author_facet |
Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan |
author_sort |
Wang, Xiaotong |
title |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_short |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_full |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_fullStr |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_sort |
evolution and functional analysis of the pif97 gene of the pacific oyster crassostrea gigas |
publisher |
Oxford University Press (OUP) |
publishDate |
2013 |
url |
http://dx.doi.org/10.1093/czoolo/59.1.109 http://academic.oup.com/cz/article-pdf/59/1/109/32969460/czoolo59-0109.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Current Zoology volume 59, issue 1, page 109-115 ISSN 2396-9814 1674-5507 |
op_doi |
https://doi.org/10.1093/czoolo/59.1.109 |
container_title |
Current Zoology |
container_volume |
59 |
container_issue |
1 |
container_start_page |
109 |
op_container_end_page |
115 |
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1778138855266844672 |