Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk
Abstract There are increasing findings of the bivalve transmissible neoplasia derived from the Pacific mussel Mytilus trossulus (MtrBTN) in populations of different Mytilus species worldwide. The Subarctic is an area where this disease has not yet been sought despite the fact that Mytilus spp. are w...
Published in: | Current Zoology |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/cz/zoac012 https://academic.oup.com/cz/advance-article-pdf/doi/10.1093/cz/zoac012/42773965/zoac012.pdf https://academic.oup.com/cz/article-pdf/69/1/91/49630007/zoac012.pdf |
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croxfordunivpr:10.1093/cz/zoac012 2024-06-09T07:49:49+00:00 Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk Skazina, Maria Odintsova, Nelly Maiorova, Mariia Frolova, Lidia Dolganova, Irina Regel, Kira Strelkov, Petr Bierne, Nicolas Russian Science Foundation 2022 http://dx.doi.org/10.1093/cz/zoac012 https://academic.oup.com/cz/advance-article-pdf/doi/10.1093/cz/zoac012/42773965/zoac012.pdf https://academic.oup.com/cz/article-pdf/69/1/91/49630007/zoac012.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by-nc/4.0/ Current Zoology volume 69, issue 1, page 91-102 ISSN 1674-5507 2396-9814 journal-article 2022 croxfordunivpr https://doi.org/10.1093/cz/zoac012 2024-05-10T13:17:10Z Abstract There are increasing findings of the bivalve transmissible neoplasia derived from the Pacific mussel Mytilus trossulus (MtrBTN) in populations of different Mytilus species worldwide. The Subarctic is an area where this disease has not yet been sought despite the fact that Mytilus spp. are widespread there, and M. trossulus itself is a boreal species. We used flow cytometry of the hemolymph, hemocytology, and histology to diagnose disseminated neoplasia in a sample of M. trossulus from Magadan in the subarctic Sea of Okhotsk. Neoplasia was identified in 11 of 214 mussels studied. Using mtDNA COI sequencing, we revealed genotypes identical or nearly identical to known MtrBTN ones in the hemolymph of most of the diseased mussels. Both MtrBTN evolutionary lineages have been identified, the widespread MtrBTN2, and MtrBTN1, so far only known from M. trossulus in British Columbia on the other side of the Pacific from Magadan. In addition, MtrBTN2 was represented by 2 common diverged mtDNA haplolineages. These conclusions were confirmed for selected cancerous mussels by molecular cloning of COI and additional nuclear and mtDNA genes. On the background of high genetic diversity, different cancers were similar in terms of ploidy (range 4.0–5.8 n) and nuclear-to-cell ratio. Our study provides the first description of neoplasia and MtrBTN in mussels from the Sea of Okhotsk and from the Subarctic, of both MtrBTN1 and MtrBTN2 in the same mussel population, and the first direct comparison between these transmissible cancers. Article in Journal/Newspaper Subarctic Oxford University Press Magadan ENVELOPE(150.803,150.803,59.564,59.564) Okhotsk Pacific Current Zoology |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract There are increasing findings of the bivalve transmissible neoplasia derived from the Pacific mussel Mytilus trossulus (MtrBTN) in populations of different Mytilus species worldwide. The Subarctic is an area where this disease has not yet been sought despite the fact that Mytilus spp. are widespread there, and M. trossulus itself is a boreal species. We used flow cytometry of the hemolymph, hemocytology, and histology to diagnose disseminated neoplasia in a sample of M. trossulus from Magadan in the subarctic Sea of Okhotsk. Neoplasia was identified in 11 of 214 mussels studied. Using mtDNA COI sequencing, we revealed genotypes identical or nearly identical to known MtrBTN ones in the hemolymph of most of the diseased mussels. Both MtrBTN evolutionary lineages have been identified, the widespread MtrBTN2, and MtrBTN1, so far only known from M. trossulus in British Columbia on the other side of the Pacific from Magadan. In addition, MtrBTN2 was represented by 2 common diverged mtDNA haplolineages. These conclusions were confirmed for selected cancerous mussels by molecular cloning of COI and additional nuclear and mtDNA genes. On the background of high genetic diversity, different cancers were similar in terms of ploidy (range 4.0–5.8 n) and nuclear-to-cell ratio. Our study provides the first description of neoplasia and MtrBTN in mussels from the Sea of Okhotsk and from the Subarctic, of both MtrBTN1 and MtrBTN2 in the same mussel population, and the first direct comparison between these transmissible cancers. |
author2 |
Bierne, Nicolas Russian Science Foundation |
format |
Article in Journal/Newspaper |
author |
Skazina, Maria Odintsova, Nelly Maiorova, Mariia Frolova, Lidia Dolganova, Irina Regel, Kira Strelkov, Petr |
spellingShingle |
Skazina, Maria Odintsova, Nelly Maiorova, Mariia Frolova, Lidia Dolganova, Irina Regel, Kira Strelkov, Petr Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
author_facet |
Skazina, Maria Odintsova, Nelly Maiorova, Mariia Frolova, Lidia Dolganova, Irina Regel, Kira Strelkov, Petr |
author_sort |
Skazina, Maria |
title |
Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
title_short |
Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
title_full |
Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
title_fullStr |
Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
title_full_unstemmed |
Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk |
title_sort |
two lineages of bivalve transmissible neoplasia affect the blue mussel mytilus trossulus gould in the subarctic sea of okhotsk |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/cz/zoac012 https://academic.oup.com/cz/advance-article-pdf/doi/10.1093/cz/zoac012/42773965/zoac012.pdf https://academic.oup.com/cz/article-pdf/69/1/91/49630007/zoac012.pdf |
long_lat |
ENVELOPE(150.803,150.803,59.564,59.564) |
geographic |
Magadan Okhotsk Pacific |
geographic_facet |
Magadan Okhotsk Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Current Zoology volume 69, issue 1, page 91-102 ISSN 1674-5507 2396-9814 |
op_rights |
https://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1093/cz/zoac012 |
container_title |
Current Zoology |
_version_ |
1801382686542004224 |