Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon
Abstract Experimental biologists now routinely quantify maximum metabolic rate (MMR) in fishes using respirometry, often with the goal of calculating aerobic scope and answering important ecological and evolutionary questions. Methods used for estimating MMR vary considerably, with the two most comm...
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Oxford University Press (OUP)
2020
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Online Access: | http://dx.doi.org/10.1093/conphys/coaa063 http://academic.oup.com/conphys/article-pdf/8/1/coaa063/33560923/coaa063.pdf |
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croxfordunivpr:10.1093/conphys/coaa063 2024-10-13T14:06:08+00:00 Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon Raby, Graham D Doherty, Claire L J Mokdad, Ali Pitcher, Trevor E Fisk, Aaron T Cooke, Steven Canada Foundation for Innovation Natural Sciences and Engineering Research Council of Canada Canada Research Chairs NSERC Post-Doctoral Fellowship 2020 http://dx.doi.org/10.1093/conphys/coaa063 http://academic.oup.com/conphys/article-pdf/8/1/coaa063/33560923/coaa063.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Conservation Physiology volume 8, issue 1 ISSN 2051-1434 journal-article 2020 croxfordunivpr https://doi.org/10.1093/conphys/coaa063 2024-09-17T04:28:57Z Abstract Experimental biologists now routinely quantify maximum metabolic rate (MMR) in fishes using respirometry, often with the goal of calculating aerobic scope and answering important ecological and evolutionary questions. Methods used for estimating MMR vary considerably, with the two most common methods being (i) the ‘chase method’, where fish are manually chased to exhaustion and immediately sealed into a respirometer for post-exercise measurement of oxygen consumption rate (ṀO2), and (ii) the ‘swim tunnel method’, whereby ṀO2 is measured while the fish swims at high speed in a swim tunnel respirometer. In this study, we compared estimates for MMR made using a 3-min exhaustive chase (followed by measurement of ṀO2 in a static respirometer) versus those made via maximal swimming in a swim tunnel respirometer. We made a total of 134 estimates of MMR using the two methods with juveniles of two salmonids (Atlantic salmon Salmo salar and Chinook salmon Oncorhynchus tshawytscha) across a 6°C temperature range. We found that the chase method underestimated ‘true’ MMR (based on the swim tunnel method) by ca. 20% in these species. The gap in MMR estimates between the two methods was not significantly affected by temperature (range of ca. 15–21°C) nor was it affected by body mass (overall range of 53.5–236 g). Our data support some previous studies that have suggested the use of a swim tunnel respirometer generates markedly higher estimates of MMR than does the chase method, at least for species in which a swim tunnel respirometer is viable (e.g. ‘athletic’ ram ventilating fishes). We recommend that the chase method could be used as a ‘proxy’ (i.e. with a correction factor) for MMR in future studies if supported by a species-specific calibration with a relevant range of temperatures, body sizes or other covariates of interest. Article in Journal/Newspaper Atlantic salmon Salmo salar Oxford University Press Conservation Physiology 8 1 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
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English |
description |
Abstract Experimental biologists now routinely quantify maximum metabolic rate (MMR) in fishes using respirometry, often with the goal of calculating aerobic scope and answering important ecological and evolutionary questions. Methods used for estimating MMR vary considerably, with the two most common methods being (i) the ‘chase method’, where fish are manually chased to exhaustion and immediately sealed into a respirometer for post-exercise measurement of oxygen consumption rate (ṀO2), and (ii) the ‘swim tunnel method’, whereby ṀO2 is measured while the fish swims at high speed in a swim tunnel respirometer. In this study, we compared estimates for MMR made using a 3-min exhaustive chase (followed by measurement of ṀO2 in a static respirometer) versus those made via maximal swimming in a swim tunnel respirometer. We made a total of 134 estimates of MMR using the two methods with juveniles of two salmonids (Atlantic salmon Salmo salar and Chinook salmon Oncorhynchus tshawytscha) across a 6°C temperature range. We found that the chase method underestimated ‘true’ MMR (based on the swim tunnel method) by ca. 20% in these species. The gap in MMR estimates between the two methods was not significantly affected by temperature (range of ca. 15–21°C) nor was it affected by body mass (overall range of 53.5–236 g). Our data support some previous studies that have suggested the use of a swim tunnel respirometer generates markedly higher estimates of MMR than does the chase method, at least for species in which a swim tunnel respirometer is viable (e.g. ‘athletic’ ram ventilating fishes). We recommend that the chase method could be used as a ‘proxy’ (i.e. with a correction factor) for MMR in future studies if supported by a species-specific calibration with a relevant range of temperatures, body sizes or other covariates of interest. |
author2 |
Cooke, Steven Canada Foundation for Innovation Natural Sciences and Engineering Research Council of Canada Canada Research Chairs NSERC Post-Doctoral Fellowship |
format |
Article in Journal/Newspaper |
author |
Raby, Graham D Doherty, Claire L J Mokdad, Ali Pitcher, Trevor E Fisk, Aaron T |
spellingShingle |
Raby, Graham D Doherty, Claire L J Mokdad, Ali Pitcher, Trevor E Fisk, Aaron T Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
author_facet |
Raby, Graham D Doherty, Claire L J Mokdad, Ali Pitcher, Trevor E Fisk, Aaron T |
author_sort |
Raby, Graham D |
title |
Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
title_short |
Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
title_full |
Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
title_fullStr |
Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
title_full_unstemmed |
Post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
title_sort |
post-exercise respirometry underestimates maximum metabolic rate in juvenile salmon |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1093/conphys/coaa063 http://academic.oup.com/conphys/article-pdf/8/1/coaa063/33560923/coaa063.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Conservation Physiology volume 8, issue 1 ISSN 2051-1434 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/conphys/coaa063 |
container_title |
Conservation Physiology |
container_volume |
8 |
container_issue |
1 |
_version_ |
1812812196700225536 |