Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19)
Abstract Objectives The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model....
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Online Access: | http://dx.doi.org/10.1093/cdn/nzz030.or04-06-19 http://academic.oup.com/cdn/article-pdf/3/Supplement_1/nzz030.OR04-06-19/28959251/nzz030.or04-06-19.pdf |
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croxfordunivpr:10.1093/cdn/nzz030.or04-06-19 2023-05-15T15:41:57+02:00 Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) Mejia, Elvira Gonzalez de Mazewski, Candice Luna-Vital, Diego Berhow, Mark 2019 http://dx.doi.org/10.1093/cdn/nzz030.or04-06-19 http://academic.oup.com/cdn/article-pdf/3/Supplement_1/nzz030.OR04-06-19/28959251/nzz030.or04-06-19.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Current Developments in Nutrition volume 3, issue Supplement_1 ISSN 2475-2991 Nutrition and Dietetics Food Science Medicine (miscellaneous) journal-article 2019 croxfordunivpr https://doi.org/10.1093/cdn/nzz030.or04-06-19 2022-04-15T06:27:54Z Abstract Objectives The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. Methods BL was obtained from an overnight soak of organic beluga black lentils (1:2 w/v) in water, filtered, frozen, and freeze-dried. The major ANC identified was delphinidin 3-O-(2-O-β-d-glucopyranosyl-α-l-arabinopyranoside (D3G). Seven and a half week old C57BL/6 mice were randomly separated into four groups: healthy control (n = 6), disease control (n = 14), BL (n = 12), and D3G (n = 12). After a one week acclimation, the mice were given treatments for 11 weeks, either placebo or BL: 600 or D3G: 41 mg/kg body weight (equivalent to D3G in a BL extract) using a voluntary jelly administration every morning throughout the study. Following the first week of treatments, mice received an AOM injection of 10 mg/kg body weight. Three cycles of DSS in water started one week later. The first cycle had 2% DSS for one week; the second and third cycles had 1%. Each cycle had two weeks of regular water post-DSS. Inflammation, progression, and immune markers were analyzed in the colon, spleen, and plasma by histology, gene expression, ELISA, and protein arrays. Results BL had total ANC concentration of 21.3 mg D3G equivalent/g dry extract and total polyphenol concentration of 264.9 mg gallic acid equivalent/g dry extract. BL group had a lower disease activity index (DAI), throughout and at the end, of 2.4 compared to 6.3 of the disease control and 4.0 of D3G group (P < 0.05). Mice in the BL group had an average of 7.8 neoplasms while the disease control had 12.8 and D3G had 12.1 (P < 0.05). BL group had a lower relative spleen weight and colon weight to length at 5 and 59 mg/cm compared to 10 and 82 mg/cm and 10 and 83 mg/cm of the disease control and D3G groups, respectively (P < 0.05). Based on colon cancer cell results, BL lowered expression of inhibitory immune markers, like PD-L1 (P < 0.05). Conclusions BL attenuated the DAI and helped mice maintain their weight throughout the AOM/DSS treatment. BL also resulted in a lower total and lower large neoplasm number, relative lower spleen weight, and colon weight to length ratio compared to the disease control. BL showed anti-carcinogenic anti-inflammatory properties. Funding Sources USDA-NIFA-HATCH project 1014457. Article in Journal/Newspaper Beluga Beluga* Oxford University Press (via Crossref) Current Developments in Nutrition 3 Supplement_1 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Nutrition and Dietetics Food Science Medicine (miscellaneous) |
spellingShingle |
Nutrition and Dietetics Food Science Medicine (miscellaneous) Mejia, Elvira Gonzalez de Mazewski, Candice Luna-Vital, Diego Berhow, Mark Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
topic_facet |
Nutrition and Dietetics Food Science Medicine (miscellaneous) |
description |
Abstract Objectives The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. Methods BL was obtained from an overnight soak of organic beluga black lentils (1:2 w/v) in water, filtered, frozen, and freeze-dried. The major ANC identified was delphinidin 3-O-(2-O-β-d-glucopyranosyl-α-l-arabinopyranoside (D3G). Seven and a half week old C57BL/6 mice were randomly separated into four groups: healthy control (n = 6), disease control (n = 14), BL (n = 12), and D3G (n = 12). After a one week acclimation, the mice were given treatments for 11 weeks, either placebo or BL: 600 or D3G: 41 mg/kg body weight (equivalent to D3G in a BL extract) using a voluntary jelly administration every morning throughout the study. Following the first week of treatments, mice received an AOM injection of 10 mg/kg body weight. Three cycles of DSS in water started one week later. The first cycle had 2% DSS for one week; the second and third cycles had 1%. Each cycle had two weeks of regular water post-DSS. Inflammation, progression, and immune markers were analyzed in the colon, spleen, and plasma by histology, gene expression, ELISA, and protein arrays. Results BL had total ANC concentration of 21.3 mg D3G equivalent/g dry extract and total polyphenol concentration of 264.9 mg gallic acid equivalent/g dry extract. BL group had a lower disease activity index (DAI), throughout and at the end, of 2.4 compared to 6.3 of the disease control and 4.0 of D3G group (P < 0.05). Mice in the BL group had an average of 7.8 neoplasms while the disease control had 12.8 and D3G had 12.1 (P < 0.05). BL group had a lower relative spleen weight and colon weight to length at 5 and 59 mg/cm compared to 10 and 82 mg/cm and 10 and 83 mg/cm of the disease control and D3G groups, respectively (P < 0.05). Based on colon cancer cell results, BL lowered expression of inhibitory immune markers, like PD-L1 (P < 0.05). Conclusions BL attenuated the DAI and helped mice maintain their weight throughout the AOM/DSS treatment. BL also resulted in a lower total and lower large neoplasm number, relative lower spleen weight, and colon weight to length ratio compared to the disease control. BL showed anti-carcinogenic anti-inflammatory properties. Funding Sources USDA-NIFA-HATCH project 1014457. |
format |
Article in Journal/Newspaper |
author |
Mejia, Elvira Gonzalez de Mazewski, Candice Luna-Vital, Diego Berhow, Mark |
author_facet |
Mejia, Elvira Gonzalez de Mazewski, Candice Luna-Vital, Diego Berhow, Mark |
author_sort |
Mejia, Elvira Gonzalez de |
title |
Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
title_short |
Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
title_full |
Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
title_fullStr |
Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
title_full_unstemmed |
Black Lentil Aqueous Extract Attenuates Colitis-Associated Colon Carcinogenesis in Mice (OR04-06-19) |
title_sort |
black lentil aqueous extract attenuates colitis-associated colon carcinogenesis in mice (or04-06-19) |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1093/cdn/nzz030.or04-06-19 http://academic.oup.com/cdn/article-pdf/3/Supplement_1/nzz030.OR04-06-19/28959251/nzz030.or04-06-19.pdf |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Current Developments in Nutrition volume 3, issue Supplement_1 ISSN 2475-2991 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/cdn/nzz030.or04-06-19 |
container_title |
Current Developments in Nutrition |
container_volume |
3 |
container_issue |
Supplement_1 |
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1766374836020445184 |