Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae)
Abstract The broom hare (Lepus castroviejoi) is a threatened Iberian endemic, for which there is limited knowledge. We use genetic non-invasive sampling (gNIS; N = 185 faeces samples) and specimens from hunting and roadkills (N = 22) in conjunction with a 15-microsatellite panel and a 541-bp fragmen...
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Online Access: | http://dx.doi.org/10.1093/biolinnean/blad080 https://academic.oup.com/biolinnean/article-pdf/141/1/148/55143306/blad080.pdf |
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croxfordunivpr:10.1093/biolinnean/blad080 2024-02-11T10:05:52+01:00 Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) Costa, José Queirós, João Ballesteros, Fernando Mucci, Nadia Souto, João Silva, Eugénio Melo-Ferreira, José Alves, Paulo Célio HybridChange Fundação para a Ciência e a Tecnologia 2023 http://dx.doi.org/10.1093/biolinnean/blad080 https://academic.oup.com/biolinnean/article-pdf/141/1/148/55143306/blad080.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights Biological Journal of the Linnean Society volume 141, issue 1, page 148-167 ISSN 0024-4066 1095-8312 Ecology, Evolution, Behavior and Systematics journal-article 2023 croxfordunivpr https://doi.org/10.1093/biolinnean/blad080 2024-01-12T10:12:09Z Abstract The broom hare (Lepus castroviejoi) is a threatened Iberian endemic, for which there is limited knowledge. We use genetic non-invasive sampling (gNIS; N = 185 faeces samples) and specimens from hunting and roadkills (N = 22) in conjunction with a 15-microsatellite panel and a 541-bp fragment of cytochrome-b to assess the genetic diversity, population structure and evolutionary history of this species. Populations from the other four European hare species were also analysed to accurately compare the genetic diversity patterns and infer admixture. Species identification from gNIS was inferred using small fragments of cytochrome-b and transferrin genes and individual identification was obtained using microsatellites. The broom hare population showed the lowest level of nuclear DNA diversity of all analysed hare species (N = 76; Na = 2.53, He = 0.186 and Fis = 0.341) and very low mitochondrial DNA diversity (N = 64; Hd = 0.743 and π = 0.01543). Only the Italian hare (L. corsicanus) showed a similar pattern of low genetic diversity. No hybridization with the neighbouring hare species was detected. However, two mitochondrial DNA lineages, corresponding to two ancient events of introgression of mountain hare (L. timidus) origin, were characterized. There was evidence for shallow spatial population differentiation of the broom hare. The described reduced genetic diversity, associated with a narrow distribution range and recent population declines, represents a risk of population extinction, and highlights the need for conservation measures of this endemic threatened hare species. Article in Journal/Newspaper mountain hare Oxford University Press Biological Journal of the Linnean Society |
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Oxford University Press |
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croxfordunivpr |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Costa, José Queirós, João Ballesteros, Fernando Mucci, Nadia Souto, João Silva, Eugénio Melo-Ferreira, José Alves, Paulo Célio Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
topic_facet |
Ecology, Evolution, Behavior and Systematics |
description |
Abstract The broom hare (Lepus castroviejoi) is a threatened Iberian endemic, for which there is limited knowledge. We use genetic non-invasive sampling (gNIS; N = 185 faeces samples) and specimens from hunting and roadkills (N = 22) in conjunction with a 15-microsatellite panel and a 541-bp fragment of cytochrome-b to assess the genetic diversity, population structure and evolutionary history of this species. Populations from the other four European hare species were also analysed to accurately compare the genetic diversity patterns and infer admixture. Species identification from gNIS was inferred using small fragments of cytochrome-b and transferrin genes and individual identification was obtained using microsatellites. The broom hare population showed the lowest level of nuclear DNA diversity of all analysed hare species (N = 76; Na = 2.53, He = 0.186 and Fis = 0.341) and very low mitochondrial DNA diversity (N = 64; Hd = 0.743 and π = 0.01543). Only the Italian hare (L. corsicanus) showed a similar pattern of low genetic diversity. No hybridization with the neighbouring hare species was detected. However, two mitochondrial DNA lineages, corresponding to two ancient events of introgression of mountain hare (L. timidus) origin, were characterized. There was evidence for shallow spatial population differentiation of the broom hare. The described reduced genetic diversity, associated with a narrow distribution range and recent population declines, represents a risk of population extinction, and highlights the need for conservation measures of this endemic threatened hare species. |
author2 |
HybridChange Fundação para a Ciência e a Tecnologia |
format |
Article in Journal/Newspaper |
author |
Costa, José Queirós, João Ballesteros, Fernando Mucci, Nadia Souto, João Silva, Eugénio Melo-Ferreira, José Alves, Paulo Célio |
author_facet |
Costa, José Queirós, João Ballesteros, Fernando Mucci, Nadia Souto, João Silva, Eugénio Melo-Ferreira, José Alves, Paulo Célio |
author_sort |
Costa, José |
title |
Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
title_short |
Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
title_full |
Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
title_fullStr |
Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
title_full_unstemmed |
Low genetic diversity and shallow population structure in the broom hare, Lepus castroviejoi (Lagomorpha: Leporidae) |
title_sort |
low genetic diversity and shallow population structure in the broom hare, lepus castroviejoi (lagomorpha: leporidae) |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1093/biolinnean/blad080 https://academic.oup.com/biolinnean/article-pdf/141/1/148/55143306/blad080.pdf |
genre |
mountain hare |
genre_facet |
mountain hare |
op_source |
Biological Journal of the Linnean Society volume 141, issue 1, page 148-167 ISSN 0024-4066 1095-8312 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1093/biolinnean/blad080 |
container_title |
Biological Journal of the Linnean Society |
_version_ |
1790603079514587136 |