Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia
Abstract The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate t...
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Oxford University Press (OUP)
2020
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Online Access: | http://dx.doi.org/10.1093/aobpla/plaa011 http://academic.oup.com/aobpla/advance-article-pdf/doi/10.1093/aobpla/plaa011/32895078/plaa011.pdf http://academic.oup.com/aobpla/article-pdf/12/2/plaa011/33025857/plaa011.pdf |
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croxfordunivpr:10.1093/aobpla/plaa011 2024-09-15T18:00:53+00:00 Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia Wilson, Julia Perry, Annika Shepherd, Jessica R Durán-Castillo, Mario Jeffree, Christopher E Cavers, Stephen Burns, Jean Natural Environment Research Council Botanical Society of Britain and Ireland Consejo Nacional de Ciencia y Tecnología 2020 http://dx.doi.org/10.1093/aobpla/plaa011 http://academic.oup.com/aobpla/advance-article-pdf/doi/10.1093/aobpla/plaa011/32895078/plaa011.pdf http://academic.oup.com/aobpla/article-pdf/12/2/plaa011/33025857/plaa011.pdf en eng Oxford University Press (OUP) http://creativecommons.org/licenses/by/4.0/ AoB PLANTS volume 12, issue 2 ISSN 2041-2851 journal-article 2020 croxfordunivpr https://doi.org/10.1093/aobpla/plaa011 2024-08-05T04:33:39Z Abstract The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate the distribution of cytotypes of Campanula rotundifolia, considering DNA variation, postglacial colonization, environmental partitioning and reproductive barriers. Cytotypes and genome size variation from across the species’ range were determined by flow cytometry and genetic variation was assessed using cpDNA markers. A common garden study examined growth and flowering phenology of tetraploid, pentaploid and hexaploid cytotypes and simulated a contact zone for investigation of reproductive barriers. Irish populations were entirely hexaploid. In Britain, hexaploids occurred mostly in western coastal populations which were allopatric with tetraploids, and in occasional sympatric inland populations. Chloroplast markers resolved distinct genetic groups, related to cytotype and geographically segregated; allopatric hexaploids were distinct from tetraploids, whereas sympatric hexaploids were not. Genome downsizing occurred between cytotypes. Progeny of open-pollinated clones from the contact zone showed that maternal tetraploids rarely produced progeny of other cytotypes, whereas the progeny of maternal hexaploids varied, with frequent pentaploids and aneuploids. The presence of distinctive hexaploid chloroplast types in Ireland, Scottish islands and western mainland Britain indicates that its establishment preceded separation of these land masses by sea-level rise c. 16 000 years BP. This group did not originate from British tetraploids and probably diverged before postglacial invasion from mainland Europe. The combination of cytotype, molecular, contact zone and common garden data shows an overall pattern reflecting postglacial colonization events, now maintained by geographic separation, together with more recent ... Article in Journal/Newspaper Campanula rotundifolia Oxford University Press AoB PLANTS 12 2 |
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Open Polar |
collection |
Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract The distribution and genetic structure of most plant species in Britain and Ireland bear the imprint of the last ice age. These patterns were largely shaped by random processes during recolonization but, in angiosperms, whole-genome duplication may also have been important. We investigate the distribution of cytotypes of Campanula rotundifolia, considering DNA variation, postglacial colonization, environmental partitioning and reproductive barriers. Cytotypes and genome size variation from across the species’ range were determined by flow cytometry and genetic variation was assessed using cpDNA markers. A common garden study examined growth and flowering phenology of tetraploid, pentaploid and hexaploid cytotypes and simulated a contact zone for investigation of reproductive barriers. Irish populations were entirely hexaploid. In Britain, hexaploids occurred mostly in western coastal populations which were allopatric with tetraploids, and in occasional sympatric inland populations. Chloroplast markers resolved distinct genetic groups, related to cytotype and geographically segregated; allopatric hexaploids were distinct from tetraploids, whereas sympatric hexaploids were not. Genome downsizing occurred between cytotypes. Progeny of open-pollinated clones from the contact zone showed that maternal tetraploids rarely produced progeny of other cytotypes, whereas the progeny of maternal hexaploids varied, with frequent pentaploids and aneuploids. The presence of distinctive hexaploid chloroplast types in Ireland, Scottish islands and western mainland Britain indicates that its establishment preceded separation of these land masses by sea-level rise c. 16 000 years BP. This group did not originate from British tetraploids and probably diverged before postglacial invasion from mainland Europe. The combination of cytotype, molecular, contact zone and common garden data shows an overall pattern reflecting postglacial colonization events, now maintained by geographic separation, together with more recent ... |
author2 |
Burns, Jean Natural Environment Research Council Botanical Society of Britain and Ireland Consejo Nacional de Ciencia y Tecnología |
format |
Article in Journal/Newspaper |
author |
Wilson, Julia Perry, Annika Shepherd, Jessica R Durán-Castillo, Mario Jeffree, Christopher E Cavers, Stephen |
spellingShingle |
Wilson, Julia Perry, Annika Shepherd, Jessica R Durán-Castillo, Mario Jeffree, Christopher E Cavers, Stephen Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
author_facet |
Wilson, Julia Perry, Annika Shepherd, Jessica R Durán-Castillo, Mario Jeffree, Christopher E Cavers, Stephen |
author_sort |
Wilson, Julia |
title |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_short |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_full |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_fullStr |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_full_unstemmed |
Invasion, isolation and evolution shape population genetic structure in Campanula rotundifolia |
title_sort |
invasion, isolation and evolution shape population genetic structure in campanula rotundifolia |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1093/aobpla/plaa011 http://academic.oup.com/aobpla/advance-article-pdf/doi/10.1093/aobpla/plaa011/32895078/plaa011.pdf http://academic.oup.com/aobpla/article-pdf/12/2/plaa011/33025857/plaa011.pdf |
genre |
Campanula rotundifolia |
genre_facet |
Campanula rotundifolia |
op_source |
AoB PLANTS volume 12, issue 2 ISSN 2041-2851 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/aobpla/plaa011 |
container_title |
AoB PLANTS |
container_volume |
12 |
container_issue |
2 |
_version_ |
1810438078807932928 |