Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean
Abstract Dinitrogen (N2) fixation is the major source of reactive nitrogen in the ocean and has been considered to occur specifically in low-latitude oligotrophic oceans. Recent studies have shown that N2 fixation also occurs in the polar regions and thus is a global process, although the physiologi...
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2023
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Online Access: | http://dx.doi.org/10.1038/s41396-023-01424-x https://www.nature.com/articles/s41396-023-01424-x.pdf https://www.nature.com/articles/s41396-023-01424-x https://academic.oup.com/ismej/article-pdf/17/8/1340/55365546/41396_2023_article_1424.pdf |
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croxfordunivpr:10.1038/s41396-023-01424-x 2024-09-15T17:52:59+00:00 Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean Shiozaki, Takuhei Nishimura, Yosuke Yoshizawa, Susumu Takami, Hideto Hamasaki, Koji Fujiwara, Amane Nishino, Shigeto Harada, Naomi Ministry of Education, Culture, Sports, Science and Technology MEXT | Japan Society for the Promotion of Science FSI project “Ocean DNA: Constructing “Bio-map” of Marine Organisms using DNA Sequence Analyses” from The University of Tokyo Ministry of Education, Culture, Sports, Science and Technology MEXT | Japan Society for the Promotion of Science FSI project “Ocean DNA: Constructing “Bio-map” of Marine Organisms using DNA Sequence Analyses” from The University of Tokyo 2023 http://dx.doi.org/10.1038/s41396-023-01424-x https://www.nature.com/articles/s41396-023-01424-x.pdf https://www.nature.com/articles/s41396-023-01424-x https://academic.oup.com/ismej/article-pdf/17/8/1340/55365546/41396_2023_article_1424.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 The ISME Journal volume 17, issue 8, page 1340-1350 ISSN 1751-7362 1751-7370 journal-article 2023 croxfordunivpr https://doi.org/10.1038/s41396-023-01424-x 2024-08-05T04:29:12Z Abstract Dinitrogen (N2) fixation is the major source of reactive nitrogen in the ocean and has been considered to occur specifically in low-latitude oligotrophic oceans. Recent studies have shown that N2 fixation also occurs in the polar regions and thus is a global process, although the physiological and ecological characteristics of polar diazotrophs are not yet known. Here, we successfully reconstructed diazotroph genomes, including that of cyanobacterium UCYN-A (Candidatus ‘Atelocyanobacterium thalassa’), from metagenome data corresponding to 111 samples isolated from the Arctic Ocean. These diazotrophs were highly abundant in the Arctic Ocean (max., 1.28% of the total microbial community), suggesting that they have important roles in the Arctic ecosystem and biogeochemical cycles. Further, we show that diazotrophs within genera Arcobacter, Psychromonas, and Oceanobacter are prevalent in the <0.2 µm fraction in the Arctic Ocean, indicating that current methods cannot capture their N2 fixation. Diazotrophs in the Arctic Ocean were either Arctic-endemic or cosmopolitan species from their global distribution patterns. Arctic-endemic diazotrophs, including Arctic UCYN-A, were similar to low-latitude-endemic and cosmopolitan diazotrophs in genome-wide function, however, they had unique gene sets (e.g., diverse aromatics degradation genes), suggesting adaptations to Arctic-specific conditions. Cosmopolitan diazotrophs were generally non-cyanobacteria and commonly had the gene that encodes the cold-inducible RNA chaperone, which presumably makes their survival possible even in deep, cold waters of global ocean and polar surface waters. This study shows global distribution pattern of diazotrophs with their genomes and provides clues to answering the question of how diazotrophs can inhabit polar waters. Article in Journal/Newspaper Arctic Arctic Ocean Oxford University Press The ISME Journal 17 8 1340 1350 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Dinitrogen (N2) fixation is the major source of reactive nitrogen in the ocean and has been considered to occur specifically in low-latitude oligotrophic oceans. Recent studies have shown that N2 fixation also occurs in the polar regions and thus is a global process, although the physiological and ecological characteristics of polar diazotrophs are not yet known. Here, we successfully reconstructed diazotroph genomes, including that of cyanobacterium UCYN-A (Candidatus ‘Atelocyanobacterium thalassa’), from metagenome data corresponding to 111 samples isolated from the Arctic Ocean. These diazotrophs were highly abundant in the Arctic Ocean (max., 1.28% of the total microbial community), suggesting that they have important roles in the Arctic ecosystem and biogeochemical cycles. Further, we show that diazotrophs within genera Arcobacter, Psychromonas, and Oceanobacter are prevalent in the <0.2 µm fraction in the Arctic Ocean, indicating that current methods cannot capture their N2 fixation. Diazotrophs in the Arctic Ocean were either Arctic-endemic or cosmopolitan species from their global distribution patterns. Arctic-endemic diazotrophs, including Arctic UCYN-A, were similar to low-latitude-endemic and cosmopolitan diazotrophs in genome-wide function, however, they had unique gene sets (e.g., diverse aromatics degradation genes), suggesting adaptations to Arctic-specific conditions. Cosmopolitan diazotrophs were generally non-cyanobacteria and commonly had the gene that encodes the cold-inducible RNA chaperone, which presumably makes their survival possible even in deep, cold waters of global ocean and polar surface waters. This study shows global distribution pattern of diazotrophs with their genomes and provides clues to answering the question of how diazotrophs can inhabit polar waters. |
author2 |
Ministry of Education, Culture, Sports, Science and Technology MEXT | Japan Society for the Promotion of Science FSI project “Ocean DNA: Constructing “Bio-map” of Marine Organisms using DNA Sequence Analyses” from The University of Tokyo Ministry of Education, Culture, Sports, Science and Technology MEXT | Japan Society for the Promotion of Science FSI project “Ocean DNA: Constructing “Bio-map” of Marine Organisms using DNA Sequence Analyses” from The University of Tokyo |
format |
Article in Journal/Newspaper |
author |
Shiozaki, Takuhei Nishimura, Yosuke Yoshizawa, Susumu Takami, Hideto Hamasaki, Koji Fujiwara, Amane Nishino, Shigeto Harada, Naomi |
spellingShingle |
Shiozaki, Takuhei Nishimura, Yosuke Yoshizawa, Susumu Takami, Hideto Hamasaki, Koji Fujiwara, Amane Nishino, Shigeto Harada, Naomi Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
author_facet |
Shiozaki, Takuhei Nishimura, Yosuke Yoshizawa, Susumu Takami, Hideto Hamasaki, Koji Fujiwara, Amane Nishino, Shigeto Harada, Naomi |
author_sort |
Shiozaki, Takuhei |
title |
Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
title_short |
Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
title_full |
Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
title_fullStr |
Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
title_full_unstemmed |
Distribution and survival strategies of endemic and cosmopolitan diazotrophs in the Arctic Ocean |
title_sort |
distribution and survival strategies of endemic and cosmopolitan diazotrophs in the arctic ocean |
publisher |
Oxford University Press (OUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1038/s41396-023-01424-x https://www.nature.com/articles/s41396-023-01424-x.pdf https://www.nature.com/articles/s41396-023-01424-x https://academic.oup.com/ismej/article-pdf/17/8/1340/55365546/41396_2023_article_1424.pdf |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
The ISME Journal volume 17, issue 8, page 1340-1350 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1038/s41396-023-01424-x |
container_title |
The ISME Journal |
container_volume |
17 |
container_issue |
8 |
container_start_page |
1340 |
op_container_end_page |
1350 |
_version_ |
1810295001449496576 |