Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling
Abstract Acidobacteriota are widespread and often abundant in marine sediments, yet their metabolic and ecological properties are poorly understood. Here, we examined metabolisms and distributions of Acidobacteriota in marine sediments of Svalbard by functional predictions from metagenome-assembled...
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Oxford University Press (OUP)
2021
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Online Access: | http://dx.doi.org/10.1038/s41396-021-00992-0 https://www.nature.com/articles/s41396-021-00992-0.pdf https://www.nature.com/articles/s41396-021-00992-0 https://academic.oup.com/ismej/article-pdf/15/11/3159/55253168/41396_2021_article_992.pdf |
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croxfordunivpr:10.1038/s41396-021-00992-0 2024-09-15T18:38:18+00:00 Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling Flieder, Mathias Buongiorno, Joy Herbold, Craig W Hausmann, Bela Rattei, Thomas Lloyd, Karen G Loy, Alexander Wasmund, Kenneth Austrian Science Fund Austrian Science Fund 2021 http://dx.doi.org/10.1038/s41396-021-00992-0 https://www.nature.com/articles/s41396-021-00992-0.pdf https://www.nature.com/articles/s41396-021-00992-0 https://academic.oup.com/ismej/article-pdf/15/11/3159/55253168/41396_2021_article_992.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 The ISME Journal volume 15, issue 11, page 3159-3180 ISSN 1751-7362 1751-7370 journal-article 2021 croxfordunivpr https://doi.org/10.1038/s41396-021-00992-0 2024-08-27T04:15:32Z Abstract Acidobacteriota are widespread and often abundant in marine sediments, yet their metabolic and ecological properties are poorly understood. Here, we examined metabolisms and distributions of Acidobacteriota in marine sediments of Svalbard by functional predictions from metagenome-assembled genomes (MAGs), amplicon sequencing of 16S rRNA and dissimilatory sulfite reductase (dsrB) genes and transcripts, and gene expression analyses of tetrathionate-amended microcosms. Acidobacteriota were the second most abundant dsrB-harboring (averaging 13%) phylum after Desulfobacterota in Svalbard sediments, and represented 4% of dsrB transcripts on average. Meta-analysis of dsrAB datasets also showed Acidobacteriota dsrAB sequences are prominent in marine sediments worldwide, averaging 15% of all sequences analysed, and represent most of the previously unclassified dsrAB in marine sediments. We propose two new Acidobacteriota genera, Candidatus Sulfomarinibacter (class Thermoanaerobaculia, “subdivision 23”) and Ca. Polarisedimenticola (“subdivision 22”), with distinct genetic properties that may explain their distributions in biogeochemically distinct sediments. Ca. Sulfomarinibacter encode flexible respiratory routes, with potential for oxygen, nitrous oxide, metal-oxide, tetrathionate, sulfur and sulfite/sulfate respiration, and possibly sulfur disproportionation. Potential nutrients and energy include cellulose, proteins, cyanophycin, hydrogen, and acetate. A Ca. Polarisedimenticola MAG encodes various enzymes to degrade proteins, and to reduce oxygen, nitrate, sulfur/polysulfide and metal-oxides. 16S rRNA gene and transcript profiling of Svalbard sediments showed Ca. Sulfomarinibacter members were relatively abundant and transcriptionally active in sulfidic fjord sediments, while Ca. Polarisedimenticola members were more relatively abundant in metal-rich fjord sediments. Overall, we reveal various physiological features of uncultured marine Acidobacteriota that indicate fundamental roles in seafloor ... Article in Journal/Newspaper Svalbard Oxford University Press The ISME Journal 15 11 3159 3180 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Acidobacteriota are widespread and often abundant in marine sediments, yet their metabolic and ecological properties are poorly understood. Here, we examined metabolisms and distributions of Acidobacteriota in marine sediments of Svalbard by functional predictions from metagenome-assembled genomes (MAGs), amplicon sequencing of 16S rRNA and dissimilatory sulfite reductase (dsrB) genes and transcripts, and gene expression analyses of tetrathionate-amended microcosms. Acidobacteriota were the second most abundant dsrB-harboring (averaging 13%) phylum after Desulfobacterota in Svalbard sediments, and represented 4% of dsrB transcripts on average. Meta-analysis of dsrAB datasets also showed Acidobacteriota dsrAB sequences are prominent in marine sediments worldwide, averaging 15% of all sequences analysed, and represent most of the previously unclassified dsrAB in marine sediments. We propose two new Acidobacteriota genera, Candidatus Sulfomarinibacter (class Thermoanaerobaculia, “subdivision 23”) and Ca. Polarisedimenticola (“subdivision 22”), with distinct genetic properties that may explain their distributions in biogeochemically distinct sediments. Ca. Sulfomarinibacter encode flexible respiratory routes, with potential for oxygen, nitrous oxide, metal-oxide, tetrathionate, sulfur and sulfite/sulfate respiration, and possibly sulfur disproportionation. Potential nutrients and energy include cellulose, proteins, cyanophycin, hydrogen, and acetate. A Ca. Polarisedimenticola MAG encodes various enzymes to degrade proteins, and to reduce oxygen, nitrate, sulfur/polysulfide and metal-oxides. 16S rRNA gene and transcript profiling of Svalbard sediments showed Ca. Sulfomarinibacter members were relatively abundant and transcriptionally active in sulfidic fjord sediments, while Ca. Polarisedimenticola members were more relatively abundant in metal-rich fjord sediments. Overall, we reveal various physiological features of uncultured marine Acidobacteriota that indicate fundamental roles in seafloor ... |
author2 |
Austrian Science Fund Austrian Science Fund |
format |
Article in Journal/Newspaper |
author |
Flieder, Mathias Buongiorno, Joy Herbold, Craig W Hausmann, Bela Rattei, Thomas Lloyd, Karen G Loy, Alexander Wasmund, Kenneth |
spellingShingle |
Flieder, Mathias Buongiorno, Joy Herbold, Craig W Hausmann, Bela Rattei, Thomas Lloyd, Karen G Loy, Alexander Wasmund, Kenneth Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
author_facet |
Flieder, Mathias Buongiorno, Joy Herbold, Craig W Hausmann, Bela Rattei, Thomas Lloyd, Karen G Loy, Alexander Wasmund, Kenneth |
author_sort |
Flieder, Mathias |
title |
Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
title_short |
Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
title_full |
Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
title_fullStr |
Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
title_full_unstemmed |
Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling |
title_sort |
novel taxa of acidobacteriota implicated in seafloor sulfur cycling |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s41396-021-00992-0 https://www.nature.com/articles/s41396-021-00992-0.pdf https://www.nature.com/articles/s41396-021-00992-0 https://academic.oup.com/ismej/article-pdf/15/11/3159/55253168/41396_2021_article_992.pdf |
genre |
Svalbard |
genre_facet |
Svalbard |
op_source |
The ISME Journal volume 15, issue 11, page 3159-3180 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1038/s41396-021-00992-0 |
container_title |
The ISME Journal |
container_volume |
15 |
container_issue |
11 |
container_start_page |
3159 |
op_container_end_page |
3180 |
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1810482666626088960 |