Global distribution of a chlorophyll f cyanobacterial marker
Abstract Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria r...
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Oxford University Press (OUP)
2020
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Online Access: | http://dx.doi.org/10.1038/s41396-020-0670-y https://www.nature.com/articles/s41396-020-0670-y.pdf https://www.nature.com/articles/s41396-020-0670-y https://academic.oup.com/ismej/article-pdf/14/9/2275/55645716/41396_2020_article_670.pdf |
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croxfordunivpr:10.1038/s41396-020-0670-y 2024-06-23T07:47:28+00:00 Global distribution of a chlorophyll f cyanobacterial marker Antonaru, Laura A Cardona, Tanai Larkum, Anthony W D Nürnberg, Dennis J RCUK | Biotechnology and Biological Sciences Research Council Imperial College London Leverhulme Trust RCUK | Biotechnology and Biological Sciences Research Council Imperial College London Leverhulme Trust 2020 http://dx.doi.org/10.1038/s41396-020-0670-y https://www.nature.com/articles/s41396-020-0670-y.pdf https://www.nature.com/articles/s41396-020-0670-y https://academic.oup.com/ismej/article-pdf/14/9/2275/55645716/41396_2020_article_670.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 The ISME Journal volume 14, issue 9, page 2275-2287 ISSN 1751-7362 1751-7370 journal-article 2020 croxfordunivpr https://doi.org/10.1038/s41396-020-0670-y 2024-06-11T04:15:22Z Abstract Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. Article in Journal/Newspaper Antarc* Antarctic Arctic Oxford University Press Antarctic Arctic The ISME Journal 14 9 2275 2287 |
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Oxford University Press |
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croxfordunivpr |
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English |
description |
Abstract Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. |
author2 |
RCUK | Biotechnology and Biological Sciences Research Council Imperial College London Leverhulme Trust RCUK | Biotechnology and Biological Sciences Research Council Imperial College London Leverhulme Trust |
format |
Article in Journal/Newspaper |
author |
Antonaru, Laura A Cardona, Tanai Larkum, Anthony W D Nürnberg, Dennis J |
spellingShingle |
Antonaru, Laura A Cardona, Tanai Larkum, Anthony W D Nürnberg, Dennis J Global distribution of a chlorophyll f cyanobacterial marker |
author_facet |
Antonaru, Laura A Cardona, Tanai Larkum, Anthony W D Nürnberg, Dennis J |
author_sort |
Antonaru, Laura A |
title |
Global distribution of a chlorophyll f cyanobacterial marker |
title_short |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full |
Global distribution of a chlorophyll f cyanobacterial marker |
title_fullStr |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full_unstemmed |
Global distribution of a chlorophyll f cyanobacterial marker |
title_sort |
global distribution of a chlorophyll f cyanobacterial marker |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41396-020-0670-y https://www.nature.com/articles/s41396-020-0670-y.pdf https://www.nature.com/articles/s41396-020-0670-y https://academic.oup.com/ismej/article-pdf/14/9/2275/55645716/41396_2020_article_670.pdf |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
The ISME Journal volume 14, issue 9, page 2275-2287 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1038/s41396-020-0670-y |
container_title |
The ISME Journal |
container_volume |
14 |
container_issue |
9 |
container_start_page |
2275 |
op_container_end_page |
2287 |
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1802651580954050560 |