Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a noness...
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Oxford University Press (OUP)
2018
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Online Access: | http://dx.doi.org/10.1038/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2.pdf https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf |
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croxfordunivpr:10.1038/s41396-018-0246-2 2024-09-30T14:39:24+00:00 Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. 2018 http://dx.doi.org/10.1038/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2.pdf https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights http://www.springer.com/tdm http://www.springer.com/tdm The ISME Journal volume 12, issue 12, page 3001-3013 ISSN 1751-7362 1751-7370 journal-article 2018 croxfordunivpr https://doi.org/10.1038/s41396-018-0246-2 2024-09-03T04:13:32Z Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi. Article in Journal/Newspaper North Atlantic Oxford University Press The ISME Journal 12 12 3001 3013 |
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Oxford University Press |
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croxfordunivpr |
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English |
description |
Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi. |
author2 |
NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. |
format |
Article in Journal/Newspaper |
author |
Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E |
spellingShingle |
Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
author_facet |
Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E |
author_sort |
Lastovetsky, Olga A |
title |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_short |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_full |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_fullStr |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_full_unstemmed |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_sort |
distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at north atlantic dunes |
publisher |
Oxford University Press (OUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1038/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2.pdf https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
The ISME Journal volume 12, issue 12, page 3001-3013 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights http://www.springer.com/tdm http://www.springer.com/tdm |
op_doi |
https://doi.org/10.1038/s41396-018-0246-2 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
12 |
container_start_page |
3001 |
op_container_end_page |
3013 |
_version_ |
1811641993257811968 |