Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes

Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a noness...

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Published in:The ISME Journal
Main Authors: Lastovetsky, Olga A, Ahn, Ezekiel, Mondo, Stephen J, Toomer, Kevin H, Zhang, Aolin, Johnson, Lynn M, Pawlowska, Teresa E
Other Authors: NSF | BIO | Division of Environmental Biology, NSF | BIO | Division of Integrative Organismal Systems, NSF | BIO | Division of Biological Infrastructure, The Frederic N. Gabler’93 Memorial Research Endowment grant.
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press (OUP) 2018
Subjects:
Online Access:http://dx.doi.org/10.1038/s41396-018-0246-2
http://www.nature.com/articles/s41396-018-0246-2
http://www.nature.com/articles/s41396-018-0246-2.pdf
https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf
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spelling croxfordunivpr:10.1038/s41396-018-0246-2 2024-09-30T14:39:24+00:00 Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. NSF | BIO | Division of Environmental Biology NSF | BIO | Division of Integrative Organismal Systems NSF | BIO | Division of Biological Infrastructure The Frederic N. Gabler’93 Memorial Research Endowment grant. 2018 http://dx.doi.org/10.1038/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2 http://www.nature.com/articles/s41396-018-0246-2.pdf https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights http://www.springer.com/tdm http://www.springer.com/tdm The ISME Journal volume 12, issue 12, page 3001-3013 ISSN 1751-7362 1751-7370 journal-article 2018 croxfordunivpr https://doi.org/10.1038/s41396-018-0246-2 2024-09-03T04:13:32Z Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi. Article in Journal/Newspaper North Atlantic Oxford University Press The ISME Journal 12 12 3001 3013
institution Open Polar
collection Oxford University Press
op_collection_id croxfordunivpr
language English
description Abstract Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria ‘Candidatus Glomeribacter gigasporarum’ (CaGg) and ‘Candidatus Moeniiplasma glomeromycotorum’ (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi.
author2 NSF | BIO | Division of Environmental Biology
NSF | BIO | Division of Integrative Organismal Systems
NSF | BIO | Division of Biological Infrastructure
The Frederic N. Gabler’93 Memorial Research Endowment grant.
NSF | BIO | Division of Environmental Biology
NSF | BIO | Division of Integrative Organismal Systems
NSF | BIO | Division of Biological Infrastructure
The Frederic N. Gabler’93 Memorial Research Endowment grant.
format Article in Journal/Newspaper
author Lastovetsky, Olga A
Ahn, Ezekiel
Mondo, Stephen J
Toomer, Kevin H
Zhang, Aolin
Johnson, Lynn M
Pawlowska, Teresa E
spellingShingle Lastovetsky, Olga A
Ahn, Ezekiel
Mondo, Stephen J
Toomer, Kevin H
Zhang, Aolin
Johnson, Lynn M
Pawlowska, Teresa E
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
author_facet Lastovetsky, Olga A
Ahn, Ezekiel
Mondo, Stephen J
Toomer, Kevin H
Zhang, Aolin
Johnson, Lynn M
Pawlowska, Teresa E
author_sort Lastovetsky, Olga A
title Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
title_short Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
title_full Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
title_fullStr Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
title_full_unstemmed Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
title_sort distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at north atlantic dunes
publisher Oxford University Press (OUP)
publishDate 2018
url http://dx.doi.org/10.1038/s41396-018-0246-2
http://www.nature.com/articles/s41396-018-0246-2
http://www.nature.com/articles/s41396-018-0246-2.pdf
https://academic.oup.com/ismej/article-pdf/12/12/3001/55851781/41396_2018_article_246.pdf
genre North Atlantic
genre_facet North Atlantic
op_source The ISME Journal
volume 12, issue 12, page 3001-3013
ISSN 1751-7362 1751-7370
op_rights https://academic.oup.com/pages/standard-publication-reuse-rights
http://www.springer.com/tdm
http://www.springer.com/tdm
op_doi https://doi.org/10.1038/s41396-018-0246-2
container_title The ISME Journal
container_volume 12
container_issue 12
container_start_page 3001
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