Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi
Abstract Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom...
Published in: | The ISME Journal |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2018
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1038/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0.pdf https://academic.oup.com/ismej/article-pdf/12/6/1594/55853365/41396_2018_article_94.pdf |
id |
croxfordunivpr:10.1038/s41396-018-0094-0 |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1038/s41396-018-0094-0 2024-05-12T08:02:06+00:00 Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I 2018 http://dx.doi.org/10.1038/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0.pdf https://academic.oup.com/ismej/article-pdf/12/6/1594/55853365/41396_2018_article_94.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by-nc-sa/4.0/ https://creativecommons.org/licenses/by-nc-sa/4.0 The ISME Journal volume 12, issue 6, page 1594-1604 ISSN 1751-7362 1751-7370 Ecology, Evolution, Behavior and Systematics Microbiology journal-article 2018 croxfordunivpr https://doi.org/10.1038/s41396-018-0094-0 2024-04-18T08:17:30Z Abstract Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. Article in Journal/Newspaper Calanus finmarchicus Copepods Oxford University Press The ISME Journal 12 6 1594 1604 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics Microbiology |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Microbiology Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
topic_facet |
Ecology, Evolution, Behavior and Systematics Microbiology |
description |
Abstract Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. |
format |
Article in Journal/Newspaper |
author |
Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I |
author_facet |
Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I |
author_sort |
Amato, Alberto |
title |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_short |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_fullStr |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full_unstemmed |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_sort |
grazer-induced transcriptomic and metabolomic response of the chain-forming diatom skeletonema marinoi |
publisher |
Oxford University Press (OUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1038/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0 http://www.nature.com/articles/s41396-018-0094-0.pdf https://academic.oup.com/ismej/article-pdf/12/6/1594/55853365/41396_2018_article_94.pdf |
genre |
Calanus finmarchicus Copepods |
genre_facet |
Calanus finmarchicus Copepods |
op_source |
The ISME Journal volume 12, issue 6, page 1594-1604 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by-nc-sa/4.0/ https://creativecommons.org/licenses/by-nc-sa/4.0 |
op_doi |
https://doi.org/10.1038/s41396-018-0094-0 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
6 |
container_start_page |
1594 |
op_container_end_page |
1604 |
_version_ |
1798844191678136320 |