Methanotrophy across a natural permafrost thaw environment
Abstract The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20–60% of this methane before emis...
Published in: | The ISME Journal |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press (OUP)
2018
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1038/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5.pdf https://academic.oup.com/ismej/article-pdf/12/10/2544/55850670/41396_2018_article_65.pdf |
id |
croxfordunivpr:10.1038/s41396-018-0065-5 |
---|---|
record_format |
openpolar |
spelling |
croxfordunivpr:10.1038/s41396-018-0065-5 2024-09-15T17:34:26+00:00 Methanotrophy across a natural permafrost thaw environment Singleton, Caitlin M McCalley, Carmody K Woodcroft, Ben J Boyd, Joel A Evans, Paul N Hodgkins, Suzanne B Chanton, Jeffrey P Frolking, Steve Crill, Patrick M Saleska, Scott R Rich, Virginia I Tyson, Gene W 2018 http://dx.doi.org/10.1038/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5.pdf https://academic.oup.com/ismej/article-pdf/12/10/2544/55850670/41396_2018_article_65.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by-nc-sa/4.0/ https://creativecommons.org/licenses/by-nc-sa/4.0 https://creativecommons.org/licenses/by-nc-sa/4.0 The ISME Journal volume 12, issue 10, page 2544-2558 ISSN 1751-7362 1751-7370 journal-article 2018 croxfordunivpr https://doi.org/10.1038/s41396-018-0065-5 2024-08-27T04:17:53Z Abstract The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20–60% of this methane before emission to the atmosphere. Despite the importance of methanotrophs to carbon cycling, these microorganisms are under-characterised and have not been studied across a natural permafrost thaw gradient. Here, we examine methanotroph communities from the active layer of a permafrost thaw gradient in Stordalen Mire (Abisko, Sweden) spanning three years, analysing 188 metagenomes and 24 metatranscriptomes paired with in situ biogeochemical data. Methanotroph community composition and activity varied significantly as thaw progressed from intact permafrost palsa, to partially thawed bog and fully thawed fen. Thirteen methanotroph population genomes were recovered, including two novel genomes belonging to the uncultivated upland soil cluster alpha (USCα) group and a novel potentially methanotrophic Hyphomicrobiaceae. Combined analysis of porewater δ13C-CH4 isotopes and methanotroph abundances showed methane oxidation was greatest below the oxic–anoxic interface in the bog. These results detail the direct effect of thaw on autochthonous methanotroph communities, and their consequent changes in population structure, activity and methane moderation potential. Article in Journal/Newspaper Abisko palsa permafrost Oxford University Press The ISME Journal 12 10 2544 2558 |
institution |
Open Polar |
collection |
Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20–60% of this methane before emission to the atmosphere. Despite the importance of methanotrophs to carbon cycling, these microorganisms are under-characterised and have not been studied across a natural permafrost thaw gradient. Here, we examine methanotroph communities from the active layer of a permafrost thaw gradient in Stordalen Mire (Abisko, Sweden) spanning three years, analysing 188 metagenomes and 24 metatranscriptomes paired with in situ biogeochemical data. Methanotroph community composition and activity varied significantly as thaw progressed from intact permafrost palsa, to partially thawed bog and fully thawed fen. Thirteen methanotroph population genomes were recovered, including two novel genomes belonging to the uncultivated upland soil cluster alpha (USCα) group and a novel potentially methanotrophic Hyphomicrobiaceae. Combined analysis of porewater δ13C-CH4 isotopes and methanotroph abundances showed methane oxidation was greatest below the oxic–anoxic interface in the bog. These results detail the direct effect of thaw on autochthonous methanotroph communities, and their consequent changes in population structure, activity and methane moderation potential. |
format |
Article in Journal/Newspaper |
author |
Singleton, Caitlin M McCalley, Carmody K Woodcroft, Ben J Boyd, Joel A Evans, Paul N Hodgkins, Suzanne B Chanton, Jeffrey P Frolking, Steve Crill, Patrick M Saleska, Scott R Rich, Virginia I Tyson, Gene W |
spellingShingle |
Singleton, Caitlin M McCalley, Carmody K Woodcroft, Ben J Boyd, Joel A Evans, Paul N Hodgkins, Suzanne B Chanton, Jeffrey P Frolking, Steve Crill, Patrick M Saleska, Scott R Rich, Virginia I Tyson, Gene W Methanotrophy across a natural permafrost thaw environment |
author_facet |
Singleton, Caitlin M McCalley, Carmody K Woodcroft, Ben J Boyd, Joel A Evans, Paul N Hodgkins, Suzanne B Chanton, Jeffrey P Frolking, Steve Crill, Patrick M Saleska, Scott R Rich, Virginia I Tyson, Gene W |
author_sort |
Singleton, Caitlin M |
title |
Methanotrophy across a natural permafrost thaw environment |
title_short |
Methanotrophy across a natural permafrost thaw environment |
title_full |
Methanotrophy across a natural permafrost thaw environment |
title_fullStr |
Methanotrophy across a natural permafrost thaw environment |
title_full_unstemmed |
Methanotrophy across a natural permafrost thaw environment |
title_sort |
methanotrophy across a natural permafrost thaw environment |
publisher |
Oxford University Press (OUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1038/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5 https://www.nature.com/articles/s41396-018-0065-5.pdf https://academic.oup.com/ismej/article-pdf/12/10/2544/55850670/41396_2018_article_65.pdf |
genre |
Abisko palsa permafrost |
genre_facet |
Abisko palsa permafrost |
op_source |
The ISME Journal volume 12, issue 10, page 2544-2558 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by-nc-sa/4.0/ https://creativecommons.org/licenses/by-nc-sa/4.0 https://creativecommons.org/licenses/by-nc-sa/4.0 |
op_doi |
https://doi.org/10.1038/s41396-018-0065-5 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
10 |
container_start_page |
2544 |
op_container_end_page |
2558 |
_version_ |
1810465924385341440 |