Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice
Abstract Diatoms are significant primary producers in sea ice, an ephemeral habitat with steep vertical gradients of temperature and salinity characterizing the ice matrix environment. To cope with the variable and challenging conditions, sea ice diatoms produce polysaccharide-rich extracellular pol...
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Oxford University Press (OUP)
2018
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croxfordunivpr:10.1038/s41396-017-0039-z 2024-06-09T07:40:34+00:00 Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice Aslam, Shazia N Strauss, Jan Thomas, David N Mock, Thomas Underwood, Graham J C 2018 http://dx.doi.org/10.1038/s41396-017-0039-z http://www.nature.com/articles/s41396-017-0039-z http://www.nature.com/articles/s41396-017-0039-z.pdf https://academic.oup.com/ismej/article-pdf/12/5/1237/55852517/41396_2017_article_39.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 The ISME Journal volume 12, issue 5, page 1237-1251 ISSN 1751-7362 1751-7370 journal-article 2018 croxfordunivpr https://doi.org/10.1038/s41396-017-0039-z 2024-05-10T13:14:03Z Abstract Diatoms are significant primary producers in sea ice, an ephemeral habitat with steep vertical gradients of temperature and salinity characterizing the ice matrix environment. To cope with the variable and challenging conditions, sea ice diatoms produce polysaccharide-rich extracellular polymeric substances (EPS) that play important roles in adhesion, cell protection, ligand binding and as organic carbon sources. Significant differences in EPS concentrations and chemical composition corresponding to temperature and salinity gradients were present in sea ice from the Weddell Sea and Eastern Antarctic regions of the Southern Ocean. To reconstruct the first metabolic pathway for EPS production in diatoms, we exposed Fragilariopsis cylindrus, a key bi-polar diatom species, to simulated sea ice formation. Transcriptome profiling under varying conditions of EPS production identified a significant number of genes and divergent alleles. Their complex differential expression patterns under simulated sea ice formation was aligned with physiological and biochemical properties of the cells, and with field measurements of sea ice EPS characteristics. Thus, the molecular complexity of the EPS pathway suggests metabolic plasticity in F. cylindrus is required to cope with the challenging conditions of the highly variable and extreme sea ice habitat. Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean Weddell Sea Oxford University Press Antarctic Southern Ocean Weddell Sea Weddell The ISME Journal 12 5 1237 1251 |
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Oxford University Press |
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description |
Abstract Diatoms are significant primary producers in sea ice, an ephemeral habitat with steep vertical gradients of temperature and salinity characterizing the ice matrix environment. To cope with the variable and challenging conditions, sea ice diatoms produce polysaccharide-rich extracellular polymeric substances (EPS) that play important roles in adhesion, cell protection, ligand binding and as organic carbon sources. Significant differences in EPS concentrations and chemical composition corresponding to temperature and salinity gradients were present in sea ice from the Weddell Sea and Eastern Antarctic regions of the Southern Ocean. To reconstruct the first metabolic pathway for EPS production in diatoms, we exposed Fragilariopsis cylindrus, a key bi-polar diatom species, to simulated sea ice formation. Transcriptome profiling under varying conditions of EPS production identified a significant number of genes and divergent alleles. Their complex differential expression patterns under simulated sea ice formation was aligned with physiological and biochemical properties of the cells, and with field measurements of sea ice EPS characteristics. Thus, the molecular complexity of the EPS pathway suggests metabolic plasticity in F. cylindrus is required to cope with the challenging conditions of the highly variable and extreme sea ice habitat. |
format |
Article in Journal/Newspaper |
author |
Aslam, Shazia N Strauss, Jan Thomas, David N Mock, Thomas Underwood, Graham J C |
spellingShingle |
Aslam, Shazia N Strauss, Jan Thomas, David N Mock, Thomas Underwood, Graham J C Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
author_facet |
Aslam, Shazia N Strauss, Jan Thomas, David N Mock, Thomas Underwood, Graham J C |
author_sort |
Aslam, Shazia N |
title |
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
title_short |
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
title_full |
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
title_fullStr |
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
title_full_unstemmed |
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice |
title_sort |
identifying metabolic pathways for production of extracellular polymeric substances by the diatom fragilariopsis cylindrus inhabiting sea ice |
publisher |
Oxford University Press (OUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1038/s41396-017-0039-z http://www.nature.com/articles/s41396-017-0039-z http://www.nature.com/articles/s41396-017-0039-z.pdf https://academic.oup.com/ismej/article-pdf/12/5/1237/55852517/41396_2017_article_39.pdf |
geographic |
Antarctic Southern Ocean Weddell Sea Weddell |
geographic_facet |
Antarctic Southern Ocean Weddell Sea Weddell |
genre |
Antarc* Antarctic Sea ice Southern Ocean Weddell Sea |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean Weddell Sea |
op_source |
The ISME Journal volume 12, issue 5, page 1237-1251 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1038/s41396-017-0039-z |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
5 |
container_start_page |
1237 |
op_container_end_page |
1251 |
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1801383945517924352 |