The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis
Abstract Symbiotic relationships between phytoplankton and N2-fixing microorganisms play a crucial role in marine ecosystems. The abundant and widespread unicellular cyanobacteria group A (UCYN-A) has recently been found to live symbiotically with a haptophyte. Here, we investigated the effect of ni...
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croxfordunivpr:10.1038/ismej.2014.253 2024-04-28T08:31:14+00:00 The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis Krupke, Andreas Mohr, Wiebke LaRoche, Julie Fuchs, Bernhard M Amann, Rudolf I Kuypers, Marcel M M 2014 http://dx.doi.org/10.1038/ismej.2014.253 http://www.nature.com/articles/ismej2014253.pdf http://www.nature.com/articles/ismej2014253 https://academic.oup.com/ismej/article-pdf/9/7/1635/56371658/41396_2015_article_bfismej2014253.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights http://www.springer.com/tdm The ISME Journal volume 9, issue 7, page 1635-1647 ISSN 1751-7362 1751-7370 Ecology, Evolution, Behavior and Systematics Microbiology journal-article 2014 croxfordunivpr https://doi.org/10.1038/ismej.2014.253 2024-04-09T07:55:16Z Abstract Symbiotic relationships between phytoplankton and N2-fixing microorganisms play a crucial role in marine ecosystems. The abundant and widespread unicellular cyanobacteria group A (UCYN-A) has recently been found to live symbiotically with a haptophyte. Here, we investigated the effect of nitrogen (N), phosphorus (P), iron (Fe) and Saharan dust additions on nitrogen (N2) fixation and primary production by the UCYN-A–haptophyte association in the subtropical eastern North Atlantic Ocean using nifH expression analysis and stable isotope incubations combined with single-cell measurements. N2 fixation by UCYN-A was stimulated by the addition of Fe and Saharan dust, although this was not reflected in the nifH expression. CO2 fixation by the haptophyte was stimulated by the addition of ammonium nitrate as well as Fe and Saharan dust. Intriguingly, the single-cell analysis using nanometer scale secondary ion mass spectrometry indicates that the increased CO2 fixation by the haptophyte in treatments without added fixed N is likely an indirect result of the positive effect of Fe and/or P on UCYN-A N2 fixation and the transfer of N2-derived N to the haptophyte. Our results reveal a direct linkage between the marine carbon and nitrogen cycles that is fuelled by the atmospheric deposition of dust. The comparison of single-cell rates suggests a tight coupling of nitrogen and carbon transfer that stays balanced even under changing nutrient regimes. However, it appears that the transfer of carbon from the haptophyte to UCYN-A requires a transfer of nitrogen from UCYN-A. This tight coupling indicates an obligate symbiosis of this globally important diazotrophic association. Article in Journal/Newspaper North Atlantic Oxford University Press The ISME Journal 9 7 1635 1647 |
institution |
Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics Microbiology |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Microbiology Krupke, Andreas Mohr, Wiebke LaRoche, Julie Fuchs, Bernhard M Amann, Rudolf I Kuypers, Marcel M M The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
topic_facet |
Ecology, Evolution, Behavior and Systematics Microbiology |
description |
Abstract Symbiotic relationships between phytoplankton and N2-fixing microorganisms play a crucial role in marine ecosystems. The abundant and widespread unicellular cyanobacteria group A (UCYN-A) has recently been found to live symbiotically with a haptophyte. Here, we investigated the effect of nitrogen (N), phosphorus (P), iron (Fe) and Saharan dust additions on nitrogen (N2) fixation and primary production by the UCYN-A–haptophyte association in the subtropical eastern North Atlantic Ocean using nifH expression analysis and stable isotope incubations combined with single-cell measurements. N2 fixation by UCYN-A was stimulated by the addition of Fe and Saharan dust, although this was not reflected in the nifH expression. CO2 fixation by the haptophyte was stimulated by the addition of ammonium nitrate as well as Fe and Saharan dust. Intriguingly, the single-cell analysis using nanometer scale secondary ion mass spectrometry indicates that the increased CO2 fixation by the haptophyte in treatments without added fixed N is likely an indirect result of the positive effect of Fe and/or P on UCYN-A N2 fixation and the transfer of N2-derived N to the haptophyte. Our results reveal a direct linkage between the marine carbon and nitrogen cycles that is fuelled by the atmospheric deposition of dust. The comparison of single-cell rates suggests a tight coupling of nitrogen and carbon transfer that stays balanced even under changing nutrient regimes. However, it appears that the transfer of carbon from the haptophyte to UCYN-A requires a transfer of nitrogen from UCYN-A. This tight coupling indicates an obligate symbiosis of this globally important diazotrophic association. |
format |
Article in Journal/Newspaper |
author |
Krupke, Andreas Mohr, Wiebke LaRoche, Julie Fuchs, Bernhard M Amann, Rudolf I Kuypers, Marcel M M |
author_facet |
Krupke, Andreas Mohr, Wiebke LaRoche, Julie Fuchs, Bernhard M Amann, Rudolf I Kuypers, Marcel M M |
author_sort |
Krupke, Andreas |
title |
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
title_short |
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
title_full |
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
title_fullStr |
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
title_full_unstemmed |
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis |
title_sort |
effect of nutrients on carbon and nitrogen fixation by the ucyn-a–haptophyte symbiosis |
publisher |
Oxford University Press (OUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1038/ismej.2014.253 http://www.nature.com/articles/ismej2014253.pdf http://www.nature.com/articles/ismej2014253 https://academic.oup.com/ismej/article-pdf/9/7/1635/56371658/41396_2015_article_bfismej2014253.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
The ISME Journal volume 9, issue 7, page 1635-1647 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights http://www.springer.com/tdm |
op_doi |
https://doi.org/10.1038/ismej.2014.253 |
container_title |
The ISME Journal |
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9 |
container_issue |
7 |
container_start_page |
1635 |
op_container_end_page |
1647 |
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