Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton
Abstract In this study, we used comparative metaproteomics to investigate the metabolic activity of microbial plankton inhabiting a seasonally hypoxic basin in the Northwest Atlantic Ocean (Bedford Basin). From winter to spring, we observed a seasonal increase in high-affinity membrane transport pro...
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Oxford University Press (OUP)
2014
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croxfordunivpr:10.1038/ismej.2013.234 2024-06-23T07:55:40+00:00 Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton Georges, Anna A El-Swais, Heba Craig, Susanne E Li, William K W Walsh, David A 2014 http://dx.doi.org/10.1038/ismej.2013.234 http://www.nature.com/articles/ismej2013234.pdf http://www.nature.com/articles/ismej2013234 https://academic.oup.com/ismej/article-pdf/8/6/1301/56287948/41396_2014_article_bfismej2013234.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 8, issue 6, page 1301-1313 ISSN 1751-7362 1751-7370 journal-article 2014 croxfordunivpr https://doi.org/10.1038/ismej.2013.234 2024-06-11T04:18:25Z Abstract In this study, we used comparative metaproteomics to investigate the metabolic activity of microbial plankton inhabiting a seasonally hypoxic basin in the Northwest Atlantic Ocean (Bedford Basin). From winter to spring, we observed a seasonal increase in high-affinity membrane transport proteins involved in scavenging of organic substrates; Rhodobacterales transporters were strongly associated with the spring phytoplankton bloom, whereas SAR11 transporters were abundant in the underlying waters. A diverse array of transporters for organic compounds were similar to the SAR324 clade, revealing an active heterotrophic lifestyle in coastal waters. Proteins involved in methanol oxidation (from the OM43 clade) and carbon monoxide (from a wide variety of bacteria) were identified throughout Bedford Basin. Metabolic niche partitioning between the SUP05 and ARCTIC96BD-19 clades, which together comprise the Gamma-proteobacterial sulfur oxidizers group was apparent. ARCTIC96BD-19 proteins involved in the transport of organic compounds indicated that in productive coastal waters this lineage tends toward a heterotrophic metabolism. In contrast, the identification of sulfur oxidation proteins from SUP05 indicated the use of reduced sulfur as an energy source in hypoxic bottom water. We identified an abundance of Marine Group I Thaumarchaeota proteins in the hypoxic deep layer, including proteins for nitrification and carbon fixation. No transporters for organic compounds were detected among the thaumarchaeal proteins, suggesting a reliance on autotrophic carbon assimilation. In summary, our analyses revealed the spatiotemporal structure of numerous metabolic activities in the coastal ocean that are central to carbon, nitrogen and sulfur cycling in the sea. Article in Journal/Newspaper Northwest Atlantic Oxford University Press Bedford ENVELOPE(-67.150,-67.150,-66.467,-66.467) The ISME Journal 8 6 1301 1313 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract In this study, we used comparative metaproteomics to investigate the metabolic activity of microbial plankton inhabiting a seasonally hypoxic basin in the Northwest Atlantic Ocean (Bedford Basin). From winter to spring, we observed a seasonal increase in high-affinity membrane transport proteins involved in scavenging of organic substrates; Rhodobacterales transporters were strongly associated with the spring phytoplankton bloom, whereas SAR11 transporters were abundant in the underlying waters. A diverse array of transporters for organic compounds were similar to the SAR324 clade, revealing an active heterotrophic lifestyle in coastal waters. Proteins involved in methanol oxidation (from the OM43 clade) and carbon monoxide (from a wide variety of bacteria) were identified throughout Bedford Basin. Metabolic niche partitioning between the SUP05 and ARCTIC96BD-19 clades, which together comprise the Gamma-proteobacterial sulfur oxidizers group was apparent. ARCTIC96BD-19 proteins involved in the transport of organic compounds indicated that in productive coastal waters this lineage tends toward a heterotrophic metabolism. In contrast, the identification of sulfur oxidation proteins from SUP05 indicated the use of reduced sulfur as an energy source in hypoxic bottom water. We identified an abundance of Marine Group I Thaumarchaeota proteins in the hypoxic deep layer, including proteins for nitrification and carbon fixation. No transporters for organic compounds were detected among the thaumarchaeal proteins, suggesting a reliance on autotrophic carbon assimilation. In summary, our analyses revealed the spatiotemporal structure of numerous metabolic activities in the coastal ocean that are central to carbon, nitrogen and sulfur cycling in the sea. |
format |
Article in Journal/Newspaper |
author |
Georges, Anna A El-Swais, Heba Craig, Susanne E Li, William K W Walsh, David A |
spellingShingle |
Georges, Anna A El-Swais, Heba Craig, Susanne E Li, William K W Walsh, David A Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
author_facet |
Georges, Anna A El-Swais, Heba Craig, Susanne E Li, William K W Walsh, David A |
author_sort |
Georges, Anna A |
title |
Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
title_short |
Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
title_full |
Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
title_fullStr |
Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
title_full_unstemmed |
Metaproteomic analysis of a winter to spring succession in coastal northwest Atlantic Ocean microbial plankton |
title_sort |
metaproteomic analysis of a winter to spring succession in coastal northwest atlantic ocean microbial plankton |
publisher |
Oxford University Press (OUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1038/ismej.2013.234 http://www.nature.com/articles/ismej2013234.pdf http://www.nature.com/articles/ismej2013234 https://academic.oup.com/ismej/article-pdf/8/6/1301/56287948/41396_2014_article_bfismej2013234.pdf |
long_lat |
ENVELOPE(-67.150,-67.150,-66.467,-66.467) |
geographic |
Bedford |
geographic_facet |
Bedford |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
The ISME Journal volume 8, issue 6, page 1301-1313 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2013.234 |
container_title |
The ISME Journal |
container_volume |
8 |
container_issue |
6 |
container_start_page |
1301 |
op_container_end_page |
1313 |
_version_ |
1802648335623913472 |