Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre
Abstract Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could beco...
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croxfordunivpr:10.1038/ismej.2012.126 2024-09-30T14:39:19+00:00 Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre Gómez-Pereira, Paola R Hartmann, Manuela Grob, Carolina Tarran, Glen A Martin, Adrian P Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V 2012 http://dx.doi.org/10.1038/ismej.2012.126 http://www.nature.com/articles/ismej2012126.pdf http://www.nature.com/articles/ismej2012126 https://academic.oup.com/ismej/article-pdf/7/3/603/56425779/41396_2013_article_bfismej2012126.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 7, issue 3, page 603-614 ISSN 1751-7362 1751-7370 journal-article 2012 croxfordunivpr https://doi.org/10.1038/ismej.2012.126 2024-09-03T04:12:51Z Abstract Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic, that is, potentially enhance uptake of scarce organic molecules using the available solar radiation to energise appropriate transport systems. Here, we assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions using 33P-ATP, 3H-ATP and 35S-methionine tracers. Light-stimulated uptake of these substrates was assessed in two dominant bacterioplankton groups discriminated by flow cytometric sorting of tracer-labelled cells and identified using catalysed reporter deposition fluorescence in situ hybridisation. One group of cells, encompassing 48% of all bacterioplankton, were identified as members of the SAR11 clade, whereas the other group (24% of all bacterioplankton) was Prochlorococcus. When exposed to light, SAR11 cells took 31% more ATP and 32% more methionine, whereas the Prochlorococcus cells took 33% more ATP and 34% more methionine. Other bacterioplankton did not demonstrate light stimulation. Thus, the SAR11 and Prochlorococcus groups, with distinctly different light-harvesting mechanisms, used light equally to enhance, by approximately one-third, the uptake of different types of organic molecules. Our findings indicate the significance of light-driven uptake of essential organic nutrients by the dominant bacterioplankton groups in the surface waters of one of the less productive, vast regions of the world’s oceans—the oligotrophic North Atlantic subtropical gyre. Article in Journal/Newspaper North Atlantic Oxford University Press The ISME Journal 7 3 603 614 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic, that is, potentially enhance uptake of scarce organic molecules using the available solar radiation to energise appropriate transport systems. Here, we assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions using 33P-ATP, 3H-ATP and 35S-methionine tracers. Light-stimulated uptake of these substrates was assessed in two dominant bacterioplankton groups discriminated by flow cytometric sorting of tracer-labelled cells and identified using catalysed reporter deposition fluorescence in situ hybridisation. One group of cells, encompassing 48% of all bacterioplankton, were identified as members of the SAR11 clade, whereas the other group (24% of all bacterioplankton) was Prochlorococcus. When exposed to light, SAR11 cells took 31% more ATP and 32% more methionine, whereas the Prochlorococcus cells took 33% more ATP and 34% more methionine. Other bacterioplankton did not demonstrate light stimulation. Thus, the SAR11 and Prochlorococcus groups, with distinctly different light-harvesting mechanisms, used light equally to enhance, by approximately one-third, the uptake of different types of organic molecules. Our findings indicate the significance of light-driven uptake of essential organic nutrients by the dominant bacterioplankton groups in the surface waters of one of the less productive, vast regions of the world’s oceans—the oligotrophic North Atlantic subtropical gyre. |
format |
Article in Journal/Newspaper |
author |
Gómez-Pereira, Paola R Hartmann, Manuela Grob, Carolina Tarran, Glen A Martin, Adrian P Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V |
spellingShingle |
Gómez-Pereira, Paola R Hartmann, Manuela Grob, Carolina Tarran, Glen A Martin, Adrian P Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
author_facet |
Gómez-Pereira, Paola R Hartmann, Manuela Grob, Carolina Tarran, Glen A Martin, Adrian P Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V |
author_sort |
Gómez-Pereira, Paola R |
title |
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
title_short |
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
title_full |
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
title_fullStr |
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
title_full_unstemmed |
Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre |
title_sort |
comparable light stimulation of organic nutrient uptake by sar11 and prochlorococcus in the north atlantic subtropical gyre |
publisher |
Oxford University Press (OUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1038/ismej.2012.126 http://www.nature.com/articles/ismej2012126.pdf http://www.nature.com/articles/ismej2012126 https://academic.oup.com/ismej/article-pdf/7/3/603/56425779/41396_2013_article_bfismej2012126.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
The ISME Journal volume 7, issue 3, page 603-614 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2012.126 |
container_title |
The ISME Journal |
container_volume |
7 |
container_issue |
3 |
container_start_page |
603 |
op_container_end_page |
614 |
_version_ |
1811641906947424256 |