High bicarbonate assimilation in the dark by Arctic bacteria
Abstract Although both autotrophic and heterotrophic microorganisms incorporate CO2 in the dark through different metabolic pathways, this process has usually been disregarded in oxic marine environments. We studied the significance and mediators of dark bicarbonate assimilation in dilution cultures...
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Oxford University Press (OUP)
2010
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croxfordunivpr:10.1038/ismej.2010.69 2024-10-13T14:04:55+00:00 High bicarbonate assimilation in the dark by Arctic bacteria Alonso-Sáez, Laura Galand, Pierre E Casamayor, Emilio O Pedrós-Alió, Carlos Bertilsson, Stefan 2010 http://dx.doi.org/10.1038/ismej.2010.69 http://www.nature.com/articles/ismej201069.pdf http://www.nature.com/articles/ismej201069 https://academic.oup.com/ismej/article-pdf/4/12/1581/56403572/41396_2010_article_bfismej201069.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 4, issue 12, page 1581-1590 ISSN 1751-7362 1751-7370 journal-article 2010 croxfordunivpr https://doi.org/10.1038/ismej.2010.69 2024-09-17T04:31:30Z Abstract Although both autotrophic and heterotrophic microorganisms incorporate CO2 in the dark through different metabolic pathways, this process has usually been disregarded in oxic marine environments. We studied the significance and mediators of dark bicarbonate assimilation in dilution cultures inoculated with winter Arctic seawater. At stationary phase, bicarbonate incorporation rates were high (0.5–2.5 μg C L−1 d−1) and correlated with rates of bacterial heterotrophic production, suggesting that most of the incorporation was due to heterotrophs. Accordingly, very few typically chemoautotrophic bacteria were detected by 16S rRNA gene cloning. The genetic analysis of the biotin carboxylase gene accC putatively involved in archaeal CO2 fixation did not yield any archaeal sequence, but amplified a variety of bacterial carboxylases involved in fatty acids biosynthesis, anaplerotic pathways and leucine catabolism. Gammaproteobacteria dominated the seawater cultures (40–70% of cell counts), followed by Betaproteobacteria and Flavobacteria as shown by catalyzed reporter deposition fluorescence in situ hybridization (CARDFISH). Both Beta- and Gammaproteobacteria were active in leucine and bicarbonate uptake, while Flavobacteria did not take up bicarbonate, as measured by microautoradiography combined with CARDFISH. Within Gammaproteobacteria, Pseudoalteromonas-Colwellia and Oleispira were very active in bicarbonate uptake (ca. 30 and 70% of active cells, respectively), while the group Arctic96B-16 did not take up bicarbonate. Our results suggest that, potentially, the incorporation of CO2 can be relevant for the metabolism of specific Arctic heterotrophic phylotypes, promoting the maintenance of their cell activity and/or longer survival under resource depleted conditions. Article in Journal/Newspaper Arctic Oxford University Press Arctic The ISME Journal 4 12 1581 1590 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Although both autotrophic and heterotrophic microorganisms incorporate CO2 in the dark through different metabolic pathways, this process has usually been disregarded in oxic marine environments. We studied the significance and mediators of dark bicarbonate assimilation in dilution cultures inoculated with winter Arctic seawater. At stationary phase, bicarbonate incorporation rates were high (0.5–2.5 μg C L−1 d−1) and correlated with rates of bacterial heterotrophic production, suggesting that most of the incorporation was due to heterotrophs. Accordingly, very few typically chemoautotrophic bacteria were detected by 16S rRNA gene cloning. The genetic analysis of the biotin carboxylase gene accC putatively involved in archaeal CO2 fixation did not yield any archaeal sequence, but amplified a variety of bacterial carboxylases involved in fatty acids biosynthesis, anaplerotic pathways and leucine catabolism. Gammaproteobacteria dominated the seawater cultures (40–70% of cell counts), followed by Betaproteobacteria and Flavobacteria as shown by catalyzed reporter deposition fluorescence in situ hybridization (CARDFISH). Both Beta- and Gammaproteobacteria were active in leucine and bicarbonate uptake, while Flavobacteria did not take up bicarbonate, as measured by microautoradiography combined with CARDFISH. Within Gammaproteobacteria, Pseudoalteromonas-Colwellia and Oleispira were very active in bicarbonate uptake (ca. 30 and 70% of active cells, respectively), while the group Arctic96B-16 did not take up bicarbonate. Our results suggest that, potentially, the incorporation of CO2 can be relevant for the metabolism of specific Arctic heterotrophic phylotypes, promoting the maintenance of their cell activity and/or longer survival under resource depleted conditions. |
format |
Article in Journal/Newspaper |
author |
Alonso-Sáez, Laura Galand, Pierre E Casamayor, Emilio O Pedrós-Alió, Carlos Bertilsson, Stefan |
spellingShingle |
Alonso-Sáez, Laura Galand, Pierre E Casamayor, Emilio O Pedrós-Alió, Carlos Bertilsson, Stefan High bicarbonate assimilation in the dark by Arctic bacteria |
author_facet |
Alonso-Sáez, Laura Galand, Pierre E Casamayor, Emilio O Pedrós-Alió, Carlos Bertilsson, Stefan |
author_sort |
Alonso-Sáez, Laura |
title |
High bicarbonate assimilation in the dark by Arctic bacteria |
title_short |
High bicarbonate assimilation in the dark by Arctic bacteria |
title_full |
High bicarbonate assimilation in the dark by Arctic bacteria |
title_fullStr |
High bicarbonate assimilation in the dark by Arctic bacteria |
title_full_unstemmed |
High bicarbonate assimilation in the dark by Arctic bacteria |
title_sort |
high bicarbonate assimilation in the dark by arctic bacteria |
publisher |
Oxford University Press (OUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1038/ismej.2010.69 http://www.nature.com/articles/ismej201069.pdf http://www.nature.com/articles/ismej201069 https://academic.oup.com/ismej/article-pdf/4/12/1581/56403572/41396_2010_article_bfismej201069.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
The ISME Journal volume 4, issue 12, page 1581-1590 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2010.69 |
container_title |
The ISME Journal |
container_volume |
4 |
container_issue |
12 |
container_start_page |
1581 |
op_container_end_page |
1590 |
_version_ |
1812810721669414912 |