Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica
Abstract Green sulfur bacteria (GSB) (Chlorobiaceae) are primary producers that are important in global carbon and sulfur cycling in natural environments. An almost complete genome sequence for a single, dominant GSB species (‘C-Ace’) was assembled from shotgun sequence data of an environmental samp...
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Oxford University Press (OUP)
2010
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Online Access: | http://dx.doi.org/10.1038/ismej.2010.28 http://www.nature.com/articles/ismej201028.pdf http://www.nature.com/articles/ismej201028 https://academic.oup.com/ismej/article-pdf/4/8/1002/56403199/41396_2010_article_bfismej201028.pdf |
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croxfordunivpr:10.1038/ismej.2010.28 2024-06-23T07:47:37+00:00 Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica Ng, Charmaine DeMaere, Matthew Z Williams, Timothy J Lauro, Federico M Raftery, Mark Gibson, John A E Andrews-Pfannkoch, Cynthia Lewis, Matt Hoffman, Jeffrey M Thomas, Torsten Cavicchioli, Ricardo 2010 http://dx.doi.org/10.1038/ismej.2010.28 http://www.nature.com/articles/ismej201028.pdf http://www.nature.com/articles/ismej201028 https://academic.oup.com/ismej/article-pdf/4/8/1002/56403199/41396_2010_article_bfismej201028.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 4, issue 8, page 1002-1019 ISSN 1751-7362 1751-7370 journal-article 2010 croxfordunivpr https://doi.org/10.1038/ismej.2010.28 2024-06-11T04:18:35Z Abstract Green sulfur bacteria (GSB) (Chlorobiaceae) are primary producers that are important in global carbon and sulfur cycling in natural environments. An almost complete genome sequence for a single, dominant GSB species (‘C-Ace’) was assembled from shotgun sequence data of an environmental sample taken from the O2–H2S interface of the water column of Ace Lake, Antarctica. Approximately 34 Mb of DNA sequence data were assembled into nine scaffolds totaling 1.79 Mb, representing approximately 19-fold coverage for the C-Ace composite genome. A high level (∼31%) of metaproteomic coverage was achieved using matched biomass. The metaproteogenomic approach provided unique insight into the protein complement required for dominating the microbial community under cold, nutrient-limited, oxygen-limited and extremely varied annual light conditions. C-Ace shows physiological traits that promote its ability to compete very effectively with other GSB and gain dominance (for example, specific bacteriochlorophylls, mechanisms of cold adaptation) as well as a syntrophic relationship with sulfate-reducing bacteria that provides a mechanism for the exchange of sulfur compounds. As a result we are able to propose an explanation of the active biological processes promoted by cold-adapted GSB and the adaptive strategies they use to thrive under the severe physiochemical conditions prevailing in polar environments. Article in Journal/Newspaper Antarc* Antarctica Oxford University Press Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) The ISME Journal 4 8 1002 1019 |
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Open Polar |
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Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract Green sulfur bacteria (GSB) (Chlorobiaceae) are primary producers that are important in global carbon and sulfur cycling in natural environments. An almost complete genome sequence for a single, dominant GSB species (‘C-Ace’) was assembled from shotgun sequence data of an environmental sample taken from the O2–H2S interface of the water column of Ace Lake, Antarctica. Approximately 34 Mb of DNA sequence data were assembled into nine scaffolds totaling 1.79 Mb, representing approximately 19-fold coverage for the C-Ace composite genome. A high level (∼31%) of metaproteomic coverage was achieved using matched biomass. The metaproteogenomic approach provided unique insight into the protein complement required for dominating the microbial community under cold, nutrient-limited, oxygen-limited and extremely varied annual light conditions. C-Ace shows physiological traits that promote its ability to compete very effectively with other GSB and gain dominance (for example, specific bacteriochlorophylls, mechanisms of cold adaptation) as well as a syntrophic relationship with sulfate-reducing bacteria that provides a mechanism for the exchange of sulfur compounds. As a result we are able to propose an explanation of the active biological processes promoted by cold-adapted GSB and the adaptive strategies they use to thrive under the severe physiochemical conditions prevailing in polar environments. |
format |
Article in Journal/Newspaper |
author |
Ng, Charmaine DeMaere, Matthew Z Williams, Timothy J Lauro, Federico M Raftery, Mark Gibson, John A E Andrews-Pfannkoch, Cynthia Lewis, Matt Hoffman, Jeffrey M Thomas, Torsten Cavicchioli, Ricardo |
spellingShingle |
Ng, Charmaine DeMaere, Matthew Z Williams, Timothy J Lauro, Federico M Raftery, Mark Gibson, John A E Andrews-Pfannkoch, Cynthia Lewis, Matt Hoffman, Jeffrey M Thomas, Torsten Cavicchioli, Ricardo Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
author_facet |
Ng, Charmaine DeMaere, Matthew Z Williams, Timothy J Lauro, Federico M Raftery, Mark Gibson, John A E Andrews-Pfannkoch, Cynthia Lewis, Matt Hoffman, Jeffrey M Thomas, Torsten Cavicchioli, Ricardo |
author_sort |
Ng, Charmaine |
title |
Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
title_short |
Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
title_full |
Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
title_fullStr |
Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
title_full_unstemmed |
Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica |
title_sort |
metaproteogenomic analysis of a dominant green sulfur bacterium from ace lake, antarctica |
publisher |
Oxford University Press (OUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1038/ismej.2010.28 http://www.nature.com/articles/ismej201028.pdf http://www.nature.com/articles/ismej201028 https://academic.oup.com/ismej/article-pdf/4/8/1002/56403199/41396_2010_article_bfismej201028.pdf |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake |
geographic_facet |
Ace Lake |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
The ISME Journal volume 4, issue 8, page 1002-1019 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2010.28 |
container_title |
The ISME Journal |
container_volume |
4 |
container_issue |
8 |
container_start_page |
1002 |
op_container_end_page |
1019 |
_version_ |
1802651750265520128 |