Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium
Abstract The genera Exiguobacterium and Psychrobacter have been frequently detected in and isolated from polar permafrost and ice. These two genera have members that can grow at temperatures as low as −5 and −10 °C, respectively. We used quantitative PCR (Q-PCR) to quantify members of these genera i...
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croxfordunivpr:10.1038/ismej.2009.25 2024-09-15T17:45:12+00:00 Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium Rodrigues, Debora F da C Jesus, Ederson Ayala-del-Río, Hector L Pellizari, Vivian H Gilichinsky, David Sepulveda-Torres, Lycely Tiedje, James M 2009 http://dx.doi.org/10.1038/ismej.2009.25 http://www.nature.com/articles/ismej200925.pdf http://www.nature.com/articles/ismej200925 https://academic.oup.com/ismej/article-pdf/3/6/658/56443880/41396_2009_article_bfismej200925.pdf en eng Oxford University Press (OUP) https://academic.oup.com/pages/standard-publication-reuse-rights The ISME Journal volume 3, issue 6, page 658-665 ISSN 1751-7362 1751-7370 journal-article 2009 croxfordunivpr https://doi.org/10.1038/ismej.2009.25 2024-06-24T04:27:55Z Abstract The genera Exiguobacterium and Psychrobacter have been frequently detected in and isolated from polar permafrost and ice. These two genera have members that can grow at temperatures as low as −5 and −10 °C, respectively. We used quantitative PCR (Q-PCR) to quantify members of these genera in 54 soil or sediment samples from polar, temperate and tropical environments to determine to what extent they are selected by cold environments. These results were further analyzed by multiple linear regression to identify the most relevant environmental factors corresponding to their distribution. Exiguobacterium was detected in all three climatic zones at similar densities, but was patchier in the temperate and tropical samples. Psychrobacter was present in almost all polar samples, was at highest densities in Antarctica sediment samples, but was in very low densities and infrequently detected in temperate and tropical soils. Clone libraries, specific for the 16S rRNA gene for each genus, were constructed from a sample from each climatic region. The clone libraries were analyzed for α and β diversities, as well as for variation in population structure by using analysis of molecular variance. Results confirm that both genera were found in all three climatic zones; however, Psychrobacter populations seemed to be much more diverse than Exiguobacterium in all three climatic zones. Furthermore, Psychrobacter populations from Antarctica are different from those in Michigan and Puerto Rico, which are similar to each other. Article in Journal/Newspaper Antarc* Antarctica Ice permafrost Oxford University Press The ISME Journal 3 6 658 665 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract The genera Exiguobacterium and Psychrobacter have been frequently detected in and isolated from polar permafrost and ice. These two genera have members that can grow at temperatures as low as −5 and −10 °C, respectively. We used quantitative PCR (Q-PCR) to quantify members of these genera in 54 soil or sediment samples from polar, temperate and tropical environments to determine to what extent they are selected by cold environments. These results were further analyzed by multiple linear regression to identify the most relevant environmental factors corresponding to their distribution. Exiguobacterium was detected in all three climatic zones at similar densities, but was patchier in the temperate and tropical samples. Psychrobacter was present in almost all polar samples, was at highest densities in Antarctica sediment samples, but was in very low densities and infrequently detected in temperate and tropical soils. Clone libraries, specific for the 16S rRNA gene for each genus, were constructed from a sample from each climatic region. The clone libraries were analyzed for α and β diversities, as well as for variation in population structure by using analysis of molecular variance. Results confirm that both genera were found in all three climatic zones; however, Psychrobacter populations seemed to be much more diverse than Exiguobacterium in all three climatic zones. Furthermore, Psychrobacter populations from Antarctica are different from those in Michigan and Puerto Rico, which are similar to each other. |
format |
Article in Journal/Newspaper |
author |
Rodrigues, Debora F da C Jesus, Ederson Ayala-del-Río, Hector L Pellizari, Vivian H Gilichinsky, David Sepulveda-Torres, Lycely Tiedje, James M |
spellingShingle |
Rodrigues, Debora F da C Jesus, Ederson Ayala-del-Río, Hector L Pellizari, Vivian H Gilichinsky, David Sepulveda-Torres, Lycely Tiedje, James M Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
author_facet |
Rodrigues, Debora F da C Jesus, Ederson Ayala-del-Río, Hector L Pellizari, Vivian H Gilichinsky, David Sepulveda-Torres, Lycely Tiedje, James M |
author_sort |
Rodrigues, Debora F |
title |
Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
title_short |
Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
title_full |
Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
title_fullStr |
Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
title_full_unstemmed |
Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium |
title_sort |
biogeography of two cold-adapted genera: psychrobacter and exiguobacterium |
publisher |
Oxford University Press (OUP) |
publishDate |
2009 |
url |
http://dx.doi.org/10.1038/ismej.2009.25 http://www.nature.com/articles/ismej200925.pdf http://www.nature.com/articles/ismej200925 https://academic.oup.com/ismej/article-pdf/3/6/658/56443880/41396_2009_article_bfismej200925.pdf |
genre |
Antarc* Antarctica Ice permafrost |
genre_facet |
Antarc* Antarctica Ice permafrost |
op_source |
The ISME Journal volume 3, issue 6, page 658-665 ISSN 1751-7362 1751-7370 |
op_rights |
https://academic.oup.com/pages/standard-publication-reuse-rights |
op_doi |
https://doi.org/10.1038/ismej.2009.25 |
container_title |
The ISME Journal |
container_volume |
3 |
container_issue |
6 |
container_start_page |
658 |
op_container_end_page |
665 |
_version_ |
1810492941574078464 |